Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lactobacillus fermentum 9-4 and its application

A technology of fermented lactobacillus and fermented food is applied in the development of functional lactic acid bacteria and the production and development of its products, and in the field of microorganisms. Human body utilization, beneficial to the preparation of fermented products, and the effect of good tolerance

Active Publication Date: 2021-03-30
GUANGXI UNIV
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the content of purine substances in various foods is difficult to accurately determine, it is difficult to control purine intake through strict diet
At the same time, strict control of diet means changes in food flavor and eating habits, which can easily lead to nutritional imbalances and are not conducive to health

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus fermentum 9-4 and its application
  • Lactobacillus fermentum 9-4 and its application
  • Lactobacillus fermentum 9-4 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Isolation, purification and preliminary screening of lactic acid bacteria strains in raw rice flour

[0054] 1. Isolation and purification of lactic acid bacteria

[0055] Weigh 2.5g of each raw rice flour sample, and dilute it with sterilized normal saline at a rate of 1:9, which is 10 -1 Dilution, take the above diluent and dilute it 10 times with normal saline, which is 10 -2 Sparsity, and so on, for serial dilutions. take 10 -1 、10 -3 、10 -5 、10 -7 Dilution, draw 100 μL respectively and spread on MRS medium, do two repetitions for each dilution, place in a 37°C incubator, cultivate for 48 hours, wait for the colonies to grow, pick the characteristic colonies of lactic acid bacteria with calcium-dissolving circles on the plate ,Such as figure 1 . Pick 3-4 single colonies from different plates and further purify by streaking.

[0056] A total of 35 strains were isolated from ten samples in Nanning City. The purified strains were stored in 15% glycerol and fro...

Embodiment 2

[0078] Molecular biology identification of primary screening lactic acid bacteria

[0079] 1. Genome Extraction

[0080] Inoculate the 8 strains that have passed the preliminary screening in the MRS medium with 1% inoculum amount, and take 3 mL of the bacterial liquid when they are cultured to the logarithmic phase. Perform genomic DNA extraction. After extraction, agarose gel electrophoresis was carried out for verification, and the verification results were as follows: Figure 6 shown.

[0081] 2. PCR amplification

[0082] Quantify the extracted gene DNA sample solution using an ultra-micro-volume full-wavelength spectrophotometer, and dilute it to 1 ng / μL, and use it as a template for PCR amplification. The specific sequences of primers 27F and 1492R are respectively SEQ ID No.2 and SEQ ID No.3 in the sequence listing. Construct a PCR reaction system according to Table 5, and use a PCR instrument for amplification. The amplification program is 94°C for 5 minutes, 94°C...

Embodiment 3

[0092] Screening of nornucleoside strains

[0093] Nucleosides in food are precursors for the formation of purines and are the main source of purines in the body. In order to screen out the strains with the function of reducing nucleoside, the metabolic ability of resting cells to inosine and guanosine was used as an index, and the inosine, guanosine, purine, etc. were detected by Waters e2695 high-performance liquid chromatography to understand each strain Ability to metabolize nucleosides.

[0094] Prepare 2mL HPLC inosine standard sample, guanosine standard sample and inosine-guanosine reaction solution system according to Table 7 below.

[0095] Table 7 HPLC 2mL solution system

[0096]

[0097] The 7 strains screened and identified were inoculated in MRS medium with 1% inoculum. After 16 hours, 10 mL of the bacterial liquid was taken at 4°C and centrifuged at 12,000 r / min for 3 minutes to collect the bacterial cells. The cells were washed with 1 mL of normal saline...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a strain of Lactobacillus fermentum 9-4 and its application. The strain was deposited in China Center for Type Culture Collection CCTCC on August 12, 2019, and the preservation number is CCTCC NO: M 2019619. The Lactobacillus fermentum is a lactic acid bacteria strain with purine-reducing ability screened from raw rice flour, a traditional food in Guangxi Zhuang area. The strain grows well on MRS agar medium and has strong acid resistance. The post-survival rate is 34%; it can grow slowly under 3% concentration of bile salt, and the survival rate is 65%. The strain has good acid resistance, bile salt resistance, and purine-reducing ability, and is suitable for the production of low-purine food and purine-reducing functional fermented food.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the field of development of functional lactic acid bacteria and production and development of products thereof. Specifically, it is a lactobacillus fermentum Lactobacillus fermentum 9-4 and an application thereof. Background technique [0002] Gout is a joint disease caused by urate deposition, which is directly related to hyperuricemia caused by purine metabolism disorder or decreased uric acid excretion. Gout patients often experience severe joint pain at night, and the pain lasts for several days or weeks, which can easily lead to redness, swelling and inflammation of the joints. After joint edema, the tissue becomes soft, which limits activities and eventually affects daily life. Gout is often accompanied by kidney disease, hyperlipidemia, hypertension, diabetes, arteriosclerosis and coronary heart disease. Therefore, gout is a metabolic disease that seriously endang...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/04A23L33/135A23C9/123C12R1/225
CPCA23C9/123A23L33/135C12N1/04C12N1/20C12N1/205C12R2001/225
Inventor 王成华卢良华刘田甜张冉朱春燕
Owner GUANGXI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com