Lactic acid bacterium separated from fulvic acid with probiotic activity

A technology for probiotics, lactic acid bacteria, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc.

Inactive Publication Date: 2018-06-29
营口富里泥炭科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Screening lactic acid bacteria from fulvic acid solution has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1: Lactic acid bacteria isolated from fulvic acid solution The fulvic acid solution was spread into the lactic acid bacteria selection medium according to different doses. The culture medium was incubated at 37°C for 1-2 days, and colonies were sorted by morphology and color. Isolated colonies were subjected to Gram staining and observed under a microscope to select colonies that were Gram-positive and had a rod-like structure. Selected colonies were grown in MRS liquid medium (pH 6.8) at 37°C for 24 hours. Colonies in cultures with a pH lower than 4.5 were selected. The colonies were cultured in MRS medium (pH 2.0), and acid-resistant lactic acid bacteria strains were isolated and identified by microbial physiological and biochemical experiments and 16S rRNA sequencing.

Embodiment 2

[0013] Example 2: Antibacterial activity to pathogenic bacteria At 37°C, culture Escherichia coli, Staphylococcus aureus, Salmonella typhimurium, Bacillus cereus, Listeria monocytogenes and Proteus mirabilis for 12 hours, and then inoculated to 6 solid beef extract peptone agar medium plates at a concentration of 105 CFU / ml. Inoculate a loop of newly screened lactic acid bacteria with an inoculation loop in each culture medium, and culture at 37°C for 24 hours. As a result, a clear growth inhibition ring was observed around the lactic acid bacteria colonies, indicating that the strains of the present invention have antibacterial activity against various pathogenic bacteria.

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PUM

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Abstract

The invention discloses a lactic acid bacterium separated from fulvic acid with probiotic activity. The lactic acid bacterium is separated from a fulvic acid solution. The lactic acid bacterium is prepared through 48 hours of culture on a lactic acid bacterium MRS agar plate culture medium at 37 DEG C, the lactic acid bacterium is grown at a temperature of 25-45 DEG C, and the pH value is 6.0-8.0.The lactic acid bacterium is facultative anaerobic and resistant to acids, has antibacterial activity for pathogenic bacteria, and also has antibacterial activity for escherichia coli, staphylococcusaureus, salmonella typhimurium, bacillus cereus, listeria monocytogenes and proteus mirabilis. The invention further provides a composition with the lactic acid bacterium of an effective dosage. Thelactic acid bacterium has excellent acid-resistant antibacterial activity, and is an excellent candidate as a seed for producing various fermented milk products and other fermentation foods.

Description

technical field [0001] The invention relates to a probiotic lactic acid bacterium, more precisely, to a novel lactic acid bacterium belonging to the genus Lactobacillus, which is isolated from fulvic acid and has excellent probiotic activity, such as acid resistance and antibacterial activity. Background technique [0002] Humic acids exist in large quantities in peat and lignite. Humic acid substances are rich in active functional groups and have certain ion exchange and adsorption properties. Fulvic acid (fulvic acid) molecules have the characteristics of small molecular weight and are easily soluble in water. Compared with humic acid, due to its relatively small molecular weight, it has excellent performance. Due to the above-mentioned characteristics of fulvic acid, its price is relatively high. Preparation and application are also more and more researched by scholars at home and abroad. There are three main methods for preparing fulvic acid: extraction, fermentation, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A23K10/18C12R1/225
CPCC12N1/20C12N1/205C12R2001/225
Inventor 朱宝伟姜莉莉朱珍仪
Owner 营口富里泥炭科技有限公司
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