Lactic acid bacterium separated from fulvic acid with probiotic activity
A technology for probiotics, lactic acid bacteria, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc.
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Embodiment 1
[0012] Example 1: Lactic acid bacteria isolated from fulvic acid solution The fulvic acid solution was spread into the lactic acid bacteria selection medium according to different doses. The culture medium was incubated at 37°C for 1-2 days, and colonies were sorted by morphology and color. Isolated colonies were subjected to Gram staining and observed under a microscope to select colonies that were Gram-positive and had a rod-like structure. Selected colonies were grown in MRS liquid medium (pH 6.8) at 37°C for 24 hours. Colonies in cultures with a pH lower than 4.5 were selected. The colonies were cultured in MRS medium (pH 2.0), and acid-resistant lactic acid bacteria strains were isolated and identified by microbial physiological and biochemical experiments and 16S rRNA sequencing.
Embodiment 2
[0013] Example 2: Antibacterial activity to pathogenic bacteria At 37°C, culture Escherichia coli, Staphylococcus aureus, Salmonella typhimurium, Bacillus cereus, Listeria monocytogenes and Proteus mirabilis for 12 hours, and then inoculated to 6 solid beef extract peptone agar medium plates at a concentration of 105 CFU / ml. Inoculate a loop of newly screened lactic acid bacteria with an inoculation loop in each culture medium, and culture at 37°C for 24 hours. As a result, a clear growth inhibition ring was observed around the lactic acid bacteria colonies, indicating that the strains of the present invention have antibacterial activity against various pathogenic bacteria.
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