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Compound micro-ecological preparation and application thereof to honey breeding process

A composite micro-ecology and preparation technology, applied in microorganism-based methods, bacteria used in food preparation, applications, etc., can solve problems such as intestinal flora micro-ecological imbalance, loss of beekeeping, antibiotic residues, etc., and achieve improvement. The effect of micro-ecological structure of flora, improving safety and enhancing immunity

Active Publication Date: 2018-12-21
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country is a big traditional beekeeping country with a history of more than 3,000 years. However, with the large-scale development of beekeeping, the incidence of bee colony diseases has also increased, and it is explosive and mixed with various pathogens. great loss to the industry
In order to control bee infectious diseases, antibiotics are still mainly used for prevention and treatment, and there are phenomena such as blind drug use and excessive use, which not only lead to the imbalance of intestinal flora microecology, enhance the drug resistance of intestinal microorganisms, and make general drugs ineffective, Moreover, antibiotics will remain in bee products, reducing the quality of bee products, affecting the health of consumers, and reducing the economic benefits of bee farms

Method used

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  • Compound micro-ecological preparation and application thereof to honey breeding process
  • Compound micro-ecological preparation and application thereof to honey breeding process
  • Compound micro-ecological preparation and application thereof to honey breeding process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Screening and Identification of the Constituent Strains of Compound Probiotics

[0022] (1) Screening of strains composed of compound microecological preparations

[0023] (1) Collection of samples

[0024] Put the healthy adult worker bees of Apis mellifera randomly picked from Luoping County, Yunnan Province into a 50mL sterilized centrifuge tube, insert carbon dioxide until the bees are comatose, fix the bees in the ultra-clean workbench, use tweezers to take out the intestines of the bees and dry them. Transfer it into a 1.5mL sterilized centrifuge tube and store it in a 4°C refrigerator for later use. All the equipment used in the experiment was sterilized with ultraviolet light and 75% ethanol.

[0025] (2) Screening of strains

[0026] Under sterile conditions, the gut samples of healthy adult worker bees of Apis mellifera were taken, added to 10 mL of PBS, homogenized for 5 min, and inoculated into MRS broth medium containing 0.1% vitamin C at a ratio of 1%, 3...

Embodiment 2

[0035] Probiotic properties of compound probiotics

[0036] (1) Bacteriostatic properties of compound probiotics

[0037] The inhibitory effect of two strains of lactic acid bacteria and their composite microecological preparations on the growth and reproduction of major intestinal pathogenic bacteria was studied by agar punching diffusion method. Lactobacillus reuteri LP4 and Lactobacillus helveticus KM7 were respectively inoculated in MRS broth medium, cultured anaerobically at 37°C for 24 hours, and the cells of Lactobacillus reuteri LP4 and Lactobacillus helveticus KM7 were inoculated with MRS broth medium The number is adjusted to 1×10 8 CFU / mL; 4°C, 5000rpm, centrifuge for 10min, collect the supernatant respectively, filter the supernatant through a 0.22μm microporous membrane, adjust the pH to 6.2 with sodium hydroxide; incubate 20μL for 24h with a concentration of 1×10 8CFU / mL indicator bacteria solution (Escherichia coli, Salmonella typhimurium, Shigella flexneri) w...

Embodiment 3

[0047] Effects of compound probiotics on growth and intestinal health of adult worker bees of Apis mellifera

[0048] (1) Effects of compound probiotics on the mortality of adult worker bees of Apis mellifera

[0049] 300 healthy adult worker bees of Apis mellifera were captured from the apiary of Yunnan Agricultural University and randomly divided into two groups. The experimental group I was the control group, which was fed with ordinary sterile sugar water, and the experimental group II was the experimental group, which was fed with compound microecology. The sugar water (1 × 10 8 CFU / mL); the control group and the experimental group were respectively raised in two self-made incubators, and sufficient sugar and water supply were ensured during the experiment; on the 3rd, 6th, and 9th day of the experimental period, statistics were made for the control group, the experimental group, and the experimental group respectively. Bee mortality in the experimental group. Experimen...

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Abstract

The invention provides a compound micro-ecological preparation and application thereof to a honey breeding process. The compound micro-ecological preparation is prepared from two types of lactic acidbacteria including lactobacillus reuteri and lactobacillus helveticus. After the two strains are cultured through an MRS agar culture medium, bacterial colonies have round shapes, are milky white andhave ordered edges, and surfaces of the bacterial colonies are smooth and convex and are not transparent; the strains have relatively good bacterium-inhibition activity and are sensitive to various antibiotics; the lactobacillus reuteri LP4 and the lactobacillus helveticus KM7 are mixed at an equal ratio to prepare the micro-ecological preparation and the micro-ecological preparation is added intosyrup for feeding honeybees, so that a bacterial colony micro-ecological structure in intestinal tracts of the honeybees can be improved, the intestinal tract immunity of the honeybees is enhanced and the survival rate of the honeybees is improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a compound microecological preparation and its application in the bee breeding process, in particular to the application of two kinds of lactic acid bacteria: Lactobacillus reuteri LP4 and Lactobacillus helveticus KM7 in the bee breeding process. Background technique [0002] Honey bee is a common beneficial insect. It can not only pollinate plants, improve the yield and quality of plant fruits and seeds, but also produce honey, collect pollen, secrete bee venom and propolis, etc. important applications in industry. my country is a big traditional beekeeping country with a history of more than 3,000 years. However, with the large-scale development of beekeeping, the incidence of bee colony diseases has also increased, and it is explosive and mixed with various pathogens. caused great losses to the industry. In order to control bee infectious diseases, antibiotics are still mainly used...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K50/90A23K10/18A23K20/163C12R1/225
CPCA23K10/18A23K20/163A23K50/90C12N1/20A23V2400/147A23V2400/173
Inventor 曹振辉林秋叶潘洪彬黄丝艳汪思凡雷清芝郭文清叶朋飞钱颖艳余行罗程
Owner YUNNAN AGRICULTURAL UNIVERSITY
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