Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A strain of Lactobacillus plantarum capable of lowering cholesterol and promoting the production of intestinal short-chain fatty acids and its application

A technology of Lactobacillus plantarum and short-chain fatty acids, applied in Lactobacillus, applications, microorganisms, etc., can solve the problems of low incidence of cardiovascular diseases, achieve the effects of lowering serum cholesterol levels, strong application potential, and alleviating liver pathological conditions

Active Publication Date: 2021-04-27
SICHUAN UNIV +1
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Herdsmen in Daocheng, Sichuan have long eaten homemade cheese with high fat content (butter milk cake, which is fermented from unrefined yak milk without adding exogenous lactic acid bacteria), but the incidence of cardiovascular disease is not high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A strain of Lactobacillus plantarum capable of lowering cholesterol and promoting the production of intestinal short-chain fatty acids and its application
  • A strain of Lactobacillus plantarum capable of lowering cholesterol and promoting the production of intestinal short-chain fatty acids and its application
  • A strain of Lactobacillus plantarum capable of lowering cholesterol and promoting the production of intestinal short-chain fatty acids and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Isolation, Screening and Molecular Biological Identification of Lactobacillus plantarum CGMCC 15463:

[0028] 1. Material preparation

[0029] Milk cake samples were provided by Kalong Village, Mula Township, Daocheng County, Sichuan Province;

[0030] The universal primer pair was synthesized by Invitrogen (Shanghai), and the 27F / 1492R sequence is as follows:

[0031] 27F: AGAGTTTGATCMTGGCTCAG

[0032] 1492R: TACGGYTACCTTGTTACGACTT

[0033] MRS liquid medium: glucose 20.0 g, peptone 10.0 g, beef extract 10.0 g, yeast powder 6.0 g, Tween 80 1.0 mL, dipotassium hydrogen phosphate 2.0 g, ammonium citrate 2.0 g, sodium acetate 5.0 g, magnesium sulfate 0.58 g, manganese sulfate 0.25 g, pure water 1 L, pH natural (add 1.5%-2.0% agar for solid medium).

[0034] 2. Specific ways

[0035]Weigh 10.0 g of the traditional yak milk cheese (milk cake) sample, stir it thoroughly in a conical flask filled with 90 mL of sterile water, and then shake it on a shaker for 60 min to co...

Embodiment 2

[0039] Detection of main fermentation characteristics of Lactobacillus plantarum CGMCC 15463

[0040] PBS buffer solution: Potassium dihydrogen phosphate 0.20 g, disodium hydrogen phosphate 1.15 g, sodium chloride 8.0 g, potassium chloride 0.2 g, add 800 mL of pure water and stir to dissolve, adjust concentrated hydrochloric acid to the corresponding pH value, and dilute to 1 L.

[0041] DMEM complete medium: DMEM stock solution, 10% fetal bovine serum, 1% 100 U / ml double antibody.

[0042] 1. Acid resistance test: Inoculate the activated third-generation lactic acid bacteria into PBS buffer solution with pH 3.5, 2.5, and 2.0 at an inoculum amount of 1%, respectively, culture at 37°C for 3 hours, and measure the number of viable lactic acid bacteria by gradient dilution plate method.

[0043] 2. Bile salt resistance test: Inoculate the activated three-generation lactic acid bacteria into the MRS liquid medium containing 0.1%, 0.2%, and 0.3% bovine bile salt at an inoculation ...

Embodiment 3

[0053] Determination of Cholesterol Degradation Ability of Lactobacillus plantarum CGMCC 15463 in Vitro

[0054] MRS-cholesterol medium: MRS liquid medium (same as Example 2) was added with 0.1% ox bile salt, and cholesterol was added to make the final concentration 100 μg / mL.

[0055] The third-generation activated lactic acid bacteria were inoculated into MRS-cholesterol medium at an inoculum of 1% by the o-phthalaldehyde method, and cultured on a shaker at 120 r / min at 37°C for 24 h. Centrifuge at 10,000 g for 10 min at 4°C, take 1 mL of the supernatant into a stoppered colorimetric tube, add 1 mL of 33% KOH and 2 mL of absolute ethanol, vortex for 90 s, place in a water bath at 37°C for 15 min, and cool to At room temperature, add 2 mL of sterile water and 3 mL of n-hexane, vortex for 60 s, let stand for 10 min, take 1 mL of the upper layer liquid in a new test tube and evaporate to dryness in a water bath at 65°C, add 2 mL of o-phthalaldehyde-glacial acetic acid solution ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of microorganisms, and specifically discloses a Lactobacillus plantarum ( Lactobacillus plantarum ) N‑1 and its applications. The Lactobacillus plantarum N-1 provided by the present invention is preserved in the General Microbiology Center of the China Microorganism Culture Collection Administration Committee, with the preservation number: CGMCC NO.15463, and the preservation date: March 20, 2018. The strain was isolated from traditional yak cheese products in Kalong Village, Mula Township, Daocheng County, Ganzi Tibetan Autonomous Prefecture, Sichuan Province. It grows well on MRS agar medium, has a certain tolerance to acids and bile salts, and is resistant to human colon cancer cells. Caco‑2 has high adhesion ability, strong cholesterol-lowering ability, and can significantly increase the production of intestinal short-chain fatty acids. It is not only of practical production value, but also of great significance to human health when applied in the field of functional foods.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Lactobacillus plantarum capable of lowering cholesterol and promoting the production of intestinal short-chain fatty acids, and in particular to the field of production and development of functional lactic acid bacteria and their products. Background technique [0002] Cardiovascular disease is the number one killer of human health and life, and hypercholesterolemia is one of the main risk factors for cardiovascular disease. With the Westernization of diet, the prevalence of dyslipidemia in my country is rapidly increasing, gradually approaching the level of Western countries, seriously endangering human health. [0003] Lactic acid bacteria (LAB) is a general term for a class of spore-free, Gram-positive bacteria that can use fermentable carbohydrates to produce large amounts of lactic acid. As a type of probiotics, lactic acid bacteria are recognized as high...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L33/135C12R1/25
CPCA23L33/135A23V2002/00C12R2001/25C12N1/205A23V2400/169A23V2200/3262A23V2200/32
Inventor 孙群刘绒梅田圆圆张国建梁鹏宽周智威
Owner SICHUAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com