Process for performing frozen separation on lactic acid bacteria and making dry powder

Abstract

The invention discloses a process for performing frozen separation on lactic acid bacteria and making dry powder. The process comprises the following steps: S1, preparation of sample bacterial liquid: cutting a lactic acid bacteria source sample into pieces, performing grinding, performing transferring into a centrifugal tube filled with 9 mL of sterile water or normal saline, performing shaking by using a vortex or a shaking table, performing uniform mixing for 10-30 minutes, namely diluting 10 <-1 > sample liquid, and continuously diluting the sample to 10 <-3 >, 10 <-4 > and 10 <-5 >; and S2, culture of lactic acid bacteria colonies: respectively sucking 0.2 mL of the 10 <-3 >, 10 <-4 > and 10 <-5 > sample solutions in the step S1, coating an MRS agar culture medium with the sample solutions, performing operating according to three plates in each gradient, and performing culturing for 24-48 hours at the temperature of 37 DEG C to obtain required lactic acid bacteria colonies. According to the invention, the MRS agar culture medium is coated with the bacterial liquid, the MRS agar culture medium contains a specific content of a freeze-drying protective agent compounded by glucose, peptone, a yeast extract and the like, and in addition, a vacuum-nitrogen charging grinding and crushing technology after freeze-drying is combined, so that the freeze-dried powder can be effectively prevented from absorbing moisture in the environment in the crushing process.

Description

technical field [0001] The invention relates to the technical field of lactic acid bacteria freeze-dried powder, in particular to a process for freeze-drying lactic acid bacteria and making dry powder. Background technique [0002] Lactic acid bacteria are a kind of fermentable carbohydrates, the general term for bacteria that mainly produce lactic acid. With the deepening of the research on the beneficial effect mechanism of lactic acid bacteria on human health and the enhancement of people's health care awareness, lactic acid bacteria have received more and more attention and research and development. It is widely used in nutrition and health care, food, medicine, feed additives and other industries. [0003] At present, lactic acid bacteria are mostly supplied to the market in the form of freeze-dried powder. The strains of lactic acid bacteria include Bifidobacterium, Lactobacillus germanus, etc. During the preparation process, lactic acid bacteria are easily degraded an...

AI Technical Summary

Problems solved by technology

[0003] At present, lactic acid bacteria are mostly supplied to the market in the form of freeze-dried powder. The strains of lactic acid bacteria include Bifidobacterium, Lactobacillus germanus, etc. During the preparation process, lactic acid bacteria are easily degraded and inactivated when they are directly freeze-dried, and the stability of live bacteria is low. , making microbial cells easy to cause damage, reducing the biological activity of cells, thereby reducing the rate of viable bacteria, thereby reducing the application of freeze-dried powder in food, medicine and additive industries. Therefore, we propose a lactic acid bacteria frozen Separation and dry powder manufacturing process to solve the above problems

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