Preparation method of lactobacillus acidophilus powder capable of increasing culturable cell content

A technology of Lactobacillus acidophilus and cell culture, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., to achieve the effect of reducing the production of bacterial cells, increasing the number of bacterial cells, and simple operation

Active Publication Date: 2022-07-01
JIANGSU WECARE BIOTECHNOLOGY CO LTD
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Problems solved by technology

This method increases the number of live bacteria of Lactobacillus acidophilus freeze-dried bacterial powder by adjusting the nutrient components of the medium, but there are still unavoidable problems caused by the production of VBNC state bacteria caused by the medium and the excessive fermentation speed. The inhibition of metabolites has a feedback effect on the bacteria to form VBNC status and other issues

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  • Preparation method of lactobacillus acidophilus powder capable of increasing culturable cell content
  • Preparation method of lactobacillus acidophilus powder capable of increasing culturable cell content

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Embodiment 1

[0043] The present embodiment provides a preparation method of Lactobacillus acidophilus bacteria powder that increases the content of culturable cells, and the preparation method comprises the following steps:

[0044] (1) Take out the glycerol tube of Lactobacillus acidophilus LA85 from the -80°C refrigerator, streak it on MRS agar medium by plate, and culture it anaerobically at 37°C for 72 h to obtain a single colony, which is the streak passage 1 Then, pick the largest colony on the plate and culture it again on MRS agar medium according to the above conditions to obtain a single colony. Record the colony size (unit: mm) with the largest diameter and the top 10 each time.

[0045] Table 1

[0046]

[0047] (2) The colony with a diameter of 4.2 mm obtained in step (1) (passage 15 times, colony 6) was inoculated into MRS liquid medium for activation and culture at 37 °C for 18 h, and then transferred with 2% of the inoculum. It was cultured in a 5 L fermenter containin...

Embodiment 2

[0050] This embodiment provides a method for preparing Lactobacillus acidophilus bacteria powder that increases the content of culturable cells. The only difference from Embodiment 1 is that in step (2), the diameter of the colony obtained in step (1) is 3.2 mm. Colonies (passage 8, colony 2) were activated for inoculation.

Embodiment 3

[0052] This embodiment provides a method for preparing Lactobacillus acidophilus bacteria powder that increases the content of culturable cells. The only difference from Embodiment 1 is that in step (2), the diameter of the colony obtained in step (1) is 3.7 mm. Colonies (passage 13, colony 5) were activated for inoculation.

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Abstract

The invention provides a preparation method of lactobacillus acidophilus powder capable of increasing culturable cell content. The preparation method comprises the following steps: (1) carrying out streak isolation culture on lactobacillus acidophilus on an MRS agar culture medium until a bacterial colony with the diameter of at least 3-5mm appears in the MRS agar culture medium; (2) inoculating the cultured bacterial colony into an MRS liquid culture medium for gradient fermentation culture, mixing an osmotic pressure regulator and/or an enzyme substance into the MRS liquid culture medium after the culture is ended, and collecting to obtain thalli; and (3) mixing the thalli with a protective agent, and carrying out electrostatic drying to obtain the lactobacillus acidophilus powder. By improving the lactobacillus acidophilus strain and the production process, the number of thalli in a VBNC state in the bacterial powder is reduced while the initial viable count of the bacterial powder is ensured, the viable count and proportion of culturable thalli are increased, and the cost of industrial production is reduced.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a preparation method of Lactobacillus acidophilus powder which can increase the content of culturable cells. Background technique [0002] The research on the culture of unculturable microorganisms and its mechanism has always been a hot and difficult point in the field of microbiology. alive but not cultivable ( viable but non-culturable , VBNC) refers to bacteria that are in a state of very low metabolic activity and do not divide, but are viable and capable of becoming culturable once revived. The number of bacteria with physiological activity, including the number of viable bacteria that can be cultivated and the number of bacteria in the VBNC state. The physiological state of VBNC exists widely in both cultured and unculturable microorganisms. [0003] Lactic acid bacteria can also form the physiological state of VBNC under certain conditions (low tempe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/38C12N1/04C12R1/23
CPCC12N1/20C12N1/38C12N1/04
Inventor 方曙光严涛陈珂可冯胜鹏朱建国程文蒋大成
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD
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