Preparation method of lactobacillus acidophilus powder capable of increasing culturable cell content
A technology of Lactobacillus acidophilus and cell culture, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., to achieve the effect of reducing the production of bacterial cells, increasing the number of bacterial cells, and simple operation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0043] The present embodiment provides a preparation method of Lactobacillus acidophilus bacteria powder that increases the content of culturable cells, and the preparation method comprises the following steps:
[0044] (1) Take out the glycerol tube of Lactobacillus acidophilus LA85 from the -80°C refrigerator, streak it on MRS agar medium by plate, and culture it anaerobically at 37°C for 72 h to obtain a single colony, which is the streak passage 1 Then, pick the largest colony on the plate and culture it again on MRS agar medium according to the above conditions to obtain a single colony. Record the colony size (unit: mm) with the largest diameter and the top 10 each time.
[0045] Table 1
[0046]
[0047] (2) The colony with a diameter of 4.2 mm obtained in step (1) (passage 15 times, colony 6) was inoculated into MRS liquid medium for activation and culture at 37 °C for 18 h, and then transferred with 2% of the inoculum. It was cultured in a 5 L fermenter containin...
Embodiment 2
[0050] This embodiment provides a method for preparing Lactobacillus acidophilus bacteria powder that increases the content of culturable cells. The only difference from Embodiment 1 is that in step (2), the diameter of the colony obtained in step (1) is 3.2 mm. Colonies (passage 8, colony 2) were activated for inoculation.
Embodiment 3
[0052] This embodiment provides a method for preparing Lactobacillus acidophilus bacteria powder that increases the content of culturable cells. The only difference from Embodiment 1 is that in step (2), the diameter of the colony obtained in step (1) is 3.7 mm. Colonies (passage 13, colony 5) were activated for inoculation.
PUM

Abstract
Description
Claims
Application Information

- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com