Special biocontrol compound microbial fertilizer for grapes and production method and application
A technology of compound microorganisms and production methods, applied in the field of compound microorganism fertilizers, can solve the problems that fertilizers cannot meet the needs of plants, fertilizer waste, environmental pollution, etc., and achieve the effects of improving micro-ecological flora, comprehensive nutrients and high added value.
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[0060] Example 1
[0061] Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus glacius were expanded cultured on an improved corn meal liquid medium, the initial pH of the medium was 6.5-7.5, the optimum fermentation temperature was 30℃~35℃, and the nitrogen source was maize Powder 150.0g / L, glucoamylase (15000u) 1.0g / L, α-amylase (1800u) 2.0g / L, KH 2 PO 4 5.0g / L, CO(NH 2 ) 2 5.0g / L, CaCl 2 5.0g / L. The effective live bacteria in the bacterial liquid reach 6×10 8 CFU / ml、5×10 8 CFU / ml and 2×10 8 CFU / ml, the adsorption medium is peat, and the mass ratio of bacteria liquid to peat is 1:3. The mass ratio of the three types of bacteria in the composite bacteria is 1:2:3, and the number of effective viable bacteria in the composite bacteria is 300-500 million CFU / g. Amino acid hydrolyzed residue was selected as the organic carrier. In view of the difference in nutritional requirements of red grapes in different growth environments and different growth periods, the mass ratio of c...
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[0064] Example 2
[0065] The initial pH of the medium is 6.5~7.5, the optimum fermentation temperature is 30℃~35℃, the nitrogen source is 200.0g / L corn flour, 1.0g / L glucoamylase (18000u), and 1.5g / L α-amylase (1800u). L, KH 2 PO 4 5.0g / L, CO(NH 2 ) 2 5.0g / L, CaCl 2 3.0g / L. After culturing Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus glaciens, the effective viable bacteria in the bacterial solution reached 5×10 8 CFU / ml, 4.5×10 8 CFU / ml and 3.2×10 8 CFU / ml, select peat as the adsorption medium. The mass ratio of the three bacterial agents in the compound bacterial agent is 2:2:3. Others are the same as in Example 1.
[0066] Table 8 Example 2 Test results of special compound microbial fertilizer for grapes
[0067]
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[0068] Example 3
[0069] Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus glacius were expanded cultured on an improved corn meal liquid medium, the initial pH of the medium was 6.5-7.5, the optimum fermentation temperature was 30℃~35℃, and the nitrogen source was maize Powder 150.0g / L, glucoamylase (20000u) 0.9g / L, α-amylase (2000u) 1.5g / L, KH 2 PO 4 7.0g / L, CO(NH 2 ) 2 7.0g / L, CaCl 2 3.0g / L. The effective live bacteria in the bacterial liquid reached 4.8×10 8 CFU / ml, 3.6×10 8 CFU / ml and 2.2×10 8 CFU / ml, the adsorption medium is peat, and the mass ratio of bacteria liquid to peat is 1:3. The mass ratio of the three inoculums in the composite inoculum is 1:2:3, and the number of effective viable bacteria in the composite inoculum is 250-420 million CFU / g. The mass ratio of the compound bacterial agent and the hydrolyzed residue is 5:90, and the inorganic fertilizer accounts for 5 parts by weight.
[0070] Table 9 Example 3 Test results of special compound microbial fer...
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