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Special biocontrol compound microbial fertilizer for grapes and production method and application

A technology of compound microorganisms and production methods, applied in the field of compound microorganism fertilizers, can solve the problems that fertilizers cannot meet the needs of plants, fertilizer waste, environmental pollution, etc., and achieve the effects of improving micro-ecological flora, comprehensive nutrients and high added value.

Active Publication Date: 2015-04-08
SHANGHAI NUOTONG INFORMATION TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, due to the traditional cultivation mode and unreasonable use of fertilizers, the growth and development of plants have been poor. Too little fertilizer application can not meet the needs of plants. At the same time, too much fertilizer application will cause waste of fertilizers and pollute the environment.

Method used

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  • Special biocontrol compound microbial fertilizer for grapes and production method and application
  • Special biocontrol compound microbial fertilizer for grapes and production method and application
  • Special biocontrol compound microbial fertilizer for grapes and production method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus colioids are expanded and cultivated with improved corn flour liquid medium, the initial pH of the medium is 6.5-7.5, the optimum fermentation temperature is 30°C-35°C, and the nitrogen source is corn Flour 150.0g / L, glucoamylase (15000u) 1.0g / L, α-amylase (1800u) 2.0g / L, KH 2 PO 4 5.0g / L, CO(NH 2 ) 2 5.0g / L, CaCl 2 5.0g / L. The effective live bacteria in the bacterial solution reached 6×10 8 CFU / ml, 5×10 8 CFU / ml and 2×10 8 CFU / ml, peat is selected as the adsorption medium, and the mass ratio of bacterial liquid to peat is 1:3. The mass ratio of the three bacterial agents in the composite bacterial agent is 1:2:3, and the effective number of viable bacteria in the composite bacterial agent is 300-500 million CFU / g. Amino acid hydrolyzed slag was selected as the organic carrier, and the mass ratio of compound bacterial agent to hydrolyzed slag was 2:90, and inorganic fertilizer accounted for 8 parts by we...

Embodiment 2

[0065] The initial pH of the medium is 6.5-7.5, the optimum fermentation temperature is 30°C-35°C, the nitrogen source is corn flour 200.0g / L, glucoamylase (18000u) 1.0g / L, α-amylase (1800u) 1.5g / L L, KH 2 PO 4 5.0g / L, CO(NH 2 ) 2 5.0g / L, CaCl 2 3.0g / L. After culturing Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus colloidus, the effective live bacteria in the bacterial liquid reached 5×10 8 CFU / ml, 4.5×10 8 CFU / ml and 3.2×10 8 CFU / ml, choose peat as the adsorption medium. The mass ratio of three kinds of bacterial agents in the composite microbial agent is 2:2:3. Others are with embodiment 1.

[0066] Table 8 Example 2 Grape Special Compound Microbial Fertilizer Test Results

[0067]

Embodiment 3

[0069] Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus colioids are expanded and cultivated with improved corn flour liquid medium, the initial pH of the medium is 6.5-7.5, the optimum fermentation temperature is 30°C-35°C, and the nitrogen source is corn Powder 150.0g / L, glucoamylase (20000u) 0.9g / L, α-amylase (2000u) 1.5g / L, KH 2 PO 4 7.0g / L, CO(NH 2 ) 2 7.0g / L, CaCl 2 3.0g / L. The effective live bacteria in the bacterial solution reached 4.8×10 8 CFU / ml, 3.6×10 8 CFU / ml and 2.2×10 8 CFU / ml, peat is selected as the adsorption medium, and the mass ratio of bacterial liquid to peat is 1:3. The mass ratio of the three bacterial agents in the composite bacterial agent is 1:2:3, and the effective number of viable bacteria in the composite bacterial agent is 250-420 million CFU / g. The mass ratio of the composite bacterial agent to the hydrolyzed slag is 5:90, and the inorganic fertilizer accounts for 5 parts by weight.

[0070] Table 9 Example 3 grape-specific c...

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Abstract

The invention discloses a special biocontrol compound microbial fertilizer for grapes. An organic raw material is amino acid hydrolysis residue, and a bacterial agent is a mixed bacterial agent of bacillus subtilis and bacillus amyloliquefaciens; the strain in the bacterial agent is subjected to factory fermentation; the initial pH value of the culture medium is 6.5-7.5, and the fermentation temperature is 30-35 DEG C; and a nitrogen source comprises 140.0-106.0g / L corn flour, 0.6-1.3g / L glucoamylase, 1.2-1.8g / L alpha-amylase, 4.0-6.5g / L KH2PO4 and 3.0-6.0g / L CO(NH2)2. The invention also discloses a production method of the compound microbial fertilizer. In the special compound microbial fertilizer for grapes, disclosed by the invention, the microorganisms and the fertilizer enter the soil at the same time and are colonized at the root of a grape plant, and the growth of pathogenic bacteria of coniothyrium diplodiella is inhibited so as to reduce the morbidity of coniothyrium diplodiella.

Description

technical field [0001] The invention relates to a compound microbial fertilizer, in particular to a special fertilizer for grapes. Background technique [0002] Grape disease is one of the most important problems in the production of grapes in my country, which causes losses to varying degrees every year. According to statistics, the loss caused by grape diseases in my country's grape production is about 30% or more every year. Different regions, different years, and different cultivation methods have different degrees of damage to grape diseases. Grasping the trend of grape disease occurrence and the problems existing in the control process is very important and meaningful for better prevention and control of grape diseases. At present, the occurrence of grape diseases in my country has the following trends: some diseases have been controlled, but some major diseases that have plagued viticulture for a long time are still serious; new diseases continue to appear, and seco...

Claims

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Application Information

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IPC IPC(8): C05F11/08C05G3/00
CPCC05F5/006C05F11/08
Inventor 闫龙翔曹亮亮周芳
Owner SHANGHAI NUOTONG INFORMATION TECH CO LTD
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