Compound microbial bacterial preparation for degrading crop straw and preparation method and application of compound microbial bacterial preparation

A technology for compounding microbial inoculants and crop straws, which is applied in the field of microbial fermentation, can solve the problems of inability to meet the production speed of straws, low utilization rate of straws, etc. Effect

Active Publication Date: 2012-09-26
DEZHOU YUANHE AGRI TECH DEVCO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the composite bacterial agent still cannot meet the straw production speed in terms of rapid decomposition of crop straw, and finding a new composite bacterial agent that can quickly decompose crop straw has become a problem to solve the current low utilization rate of straw.

Method used

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  • Compound microbial bacterial preparation for degrading crop straw and preparation method and application of compound microbial bacterial preparation
  • Compound microbial bacterial preparation for degrading crop straw and preparation method and application of compound microbial bacterial preparation
  • Compound microbial bacterial preparation for degrading crop straw and preparation method and application of compound microbial bacterial preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A preparation method for a compound microbial bacterial agent for degrading crop stalks, comprising the following steps:

[0051] (1) Inoculate Bacillus subtilis CICC10088 in LB liquid medium, culture it at 37°C for 24 hours, then inoculate Bacillus subtilis in solid medium A at an inoculation amount of 10wt%, and culture it at 37°C for 3 days, Prepared 10 live bacteria containing Bacillus subtilis CICC10088 10 Each / g of solid microbial agent A;

[0052] (2) Inoculate Bacillus cereus ACCC03002 in LB liquid medium, culture it at 30°C for 24 hours, then inoculate Bacillus cereus in solid medium B at an inoculation amount of 10wt%, and culture it at 30°C for 3 days, the number of live bacteria containing Bacillus cereus ACCC03002 was 8×10 9 A / g of solid bacterial agent B;

[0053] (3) Inoculate Aspergillus niger CICC41130 on PDA solid medium, culture at 28°C for 60 hours, add sterile water to adjust the spore concentration to 10 8 Individual / ml, then inoculate Aspergil...

Embodiment 2

[0070] Adopt the method as described in Example 1, the difference is that step (9) solid bacterial agent A, solid bacterial agent B, solid bacterial agent C, solid bacterial agent D, solid bacterial agent E, solid bacterial agent F, solid bacterial agent The mixing weight ratio of bacterial agent G and solid bacterial agent H is 3:1:3:1:3:1:3:1.

[0071] In the prepared composite microbial agent for degrading crop stalks, each gram of the composite microbial agent contains: Bacillus subtilis (Bacillus subtilis) viable bacteria count 1.87×10 9 pcs, Bacillus cereus (Bacillus cereus) viable count 5×10 8 , Aspergillus niger (Aspergillus niger) spore number 3.75×10 8 1. Number of spores of Aspergillus niger 1.25×10 8 , the number of spores of Trichoderma reesei 2.81×10 8 The number of spores of Trichoderma viride 9.37×10 7 1. The number of spores of Sporotrichum thermophilic 1.87×10 8 1. Number of spores of Phanerochaete chrysosporium 6.25×10 7 indivual.

Embodiment 3

[0073] Adopt the method as described in Example 1, the difference is that step (9) solid bacterial agent A, solid bacterial agent B, solid bacterial agent C, solid bacterial agent D, solid bacterial agent E, solid bacterial agent F, solid bacterial agent The mixing weight ratio of agent G and solid bacterial agent H is 2:1:1:1:2:1:1:1.

[0074] In the prepared composite microbial agent for degrading crop stalks, each gram of the composite microbial agent contains: Bacillus subtilis (Bacillus subtilis) viable bacteria count 2×10 9 , Bacillus cereus (Bacillus cereus) live bacteria count 8×10 8 , Aspergillus niger (Aspergillus niger) spore count 2×10 8 , the number of spores of Aspergillus niger 2×10 8 , Trichoderma reesei spore number 3×10 8 1. The number of spores of Trichoderma viride 1.5×10 8 1, the number of spores of Sporotrichum thermophilic 10 8 Phanerochaete chrysosporium spores 10 8 indivual.

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Abstract

The invention relates to a compound microbial bacterial preparation for degrading crop straw and a preparation method and application of the compound microbial bacterial preparation. The compound microbial bacterial preparation is prepared from bacillus subtilis, bacillus cereus, aspergillus niger, aspergillus flavus, trichoderma reesei, trichoderma longibrachiatum, sporotrichum thermophile and phanerochaete chrysosporium through solid fermentation. The compound microbial bacterial preparation can shorten the decomposition time and improve the decomposition efficiency of the crop straw. Microorganisms generate various effective metabolic products with nitrogen fixation and phosphate solubilizing functions and a high disease and pest resistance effect in the fermentation process, so that the compound microbial bacterial preparation is favorable for improving the soil structure and the yield and the quality of crops.

Description

technical field [0001] The invention relates to a compound microbial bacterial agent for efficiently degrading crop stalks, a preparation method and application thereof, and belongs to the technical field of microbial fermentation. Background technique [0002] my country is a large agricultural country, with a large output, wide distribution and variety of crop straws. Since the reform and opening up, with the increase of the central government's support for agriculture, my country's agriculture has developed rapidly, and the output of straw has exceeded 700 million tons. pollute the environment and cause serious harm to human health. Another part of the traditional application mode of using straw directly as fertilizer and feed without any treatment seriously restricts the further improvement of the conversion rate of straw. is of great significance. [0003] At present, the organic fertilizer produced with straw as raw material has been widely promoted in rural areas. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/14C12N1/20C05F17/00C12R1/125C12R1/085C12R1/685C12R1/67C12R1/885C12R1/645
CPCY02W30/40
Inventor 展彬杨瑞
Owner DEZHOU YUANHE AGRI TECH DEVCO
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