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Rhodococcus sp., lipopeptid extra-cellular bio-demulsifier, preparation method and application thereof

A rhodococcus and demulsifier technology, applied in the fields of petrochemical and industrial microbiology, can solve the problems of high extraction cost of cell wall-bound products and impact on oil quality, solve the problems of transportation and storage, and solve the high extraction cost , the effect of occupying a small volume

Inactive Publication Date: 2010-06-09
TONGJI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the above problems, the present invention aims to study a lipopeptide extracellular biological demulsifier to solve the problem of the impact on the quality of the oil and the high cost of extracting the cell wall-bound product when the full culture solution is added.

Method used

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  • Rhodococcus sp., lipopeptid extra-cellular bio-demulsifier, preparation method and application thereof
  • Rhodococcus sp., lipopeptid extra-cellular bio-demulsifier, preparation method and application thereof
  • Rhodococcus sp., lipopeptid extra-cellular bio-demulsifier, preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Demulsification effect of broth culture on kerosene model emulsion:

[0038] The Rhodococcus S-SL-2 obtained by screening was inserted into the broth medium, and cultured in a shaker with a temperature of 35°C and a rotation speed of 140rpm for 3 days, and the whole culture solution was centrifuged at 10000rpm for 20min, and the supernatant was poured out. The centrifuged bacteria were suspended and restored to the original volume with distilled water to make a bacterial suspension, and the whole culture medium, supernatant, and bacterial suspension were respectively added to the W / O and O / W kerosene model emulsions, and the breaking effects of the three were compared. Demulsification effect, and compared with the demulsification effect of no sample and blank broth medium, the temperature of the demulsification test was 35 °C, and the dosage was 10% (V / V). The results are shown in figure 2 , image 3 .

[0039] from figure 2 It can be seen that the demulsification ...

Embodiment 2

[0042] Demulsification effect of paraffin culture on kerosene model emulsion:

[0043] Insert the Rhodococcus S-SL-2 obtained from the screening into paraffin medium, and culture it in a shaker with a temperature of 35°C and a rotation speed of 140rpm for 7 days, and centrifuge the whole culture solution at 10,000rpm for 20min to remove the remaining paraffin in the upper layer and the middle layer. Finally, the centrifuged bacteria were suspended and reduced to the original volume with distilled water to make a bacterial suspension, and the whole culture medium, middle layer liquid, and bacterial suspension were respectively added to the W / O and O / W kerosene model emulsions, and the effects of the three were compared. Demulsification effect, and compared with the demulsification effect without adding any sample, the temperature of the demulsification test is 35 ℃, the dosage is 10% (V / V), the results are shown in Figure 4 , Figure 5 .

[0044] from Figure 4 It can be se...

Embodiment 3

[0047] Demulsification effect of biological demulsifier on W / O kerosene model emulsion:

[0048] The supernatant and middle layer obtained from S-SL-2 in broth medium and paraffin medium were respectively adjusted to pH 2.0 with 6mol / L HCl, stored at 4°C overnight, and then centrifuged at 10,000rpm for 20min , the supernatant was discarded to obtain the primary product in the form of flocculent precipitates, and after 24 hours of freeze-drying, the final biological dry powder demulsifier was obtained. Dissolve the biodemulsifier with a small amount of alkaline solution, adjust the pH to 7.0 with NaOH, and prepare the concentration with distilled water. Test the demulsification effect of biological demulsifiers at different dosages, the results are shown in Image 6 , Figure 7 .

[0049] from Image 6 It can be seen from the figure that the demulsification rate of the W / O kerosene model emulsion is increased with the increase of the dosage of the biological demulsifier ext...

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Abstract

The invention relates to Rhodococcus sp., lipopeptid extra-cellular bio-demulsifier produced by culturing the Rhodococcus sp. and an application thereof. The dosage of the bio-demulsifier is 100-200mg / L, and the demulsification rate of the W / O type kerosene model emulsion can be 72.7-89.1% within 24h when the demulsification test temperature is 35 DEG C. When the bio-demulsifier is used in thin oil extraction liquid in ocean extraction plants of Shengli Oil Field (SLOF) and the dosage is 90-140mg / L, the demulsification rate can achieve 60.0-62.5% within 6.5h which is further superior to the chemical demulsifier used on site. The extra-cellular bio-demulsifier has the advantages of convenient extraction, low production cost, easy storage and transportation, and can solve the problems of influenced oil product quality and high extraction cost of cell wall combined products when full culture liquid is added.

Description

technical field [0001] The invention belongs to the field of petrochemical industry and industrial microorganism. Background technique [0002] At present, most of my country's oil fields use water injection to extract crude oil, so in the process of crude oil extraction, the extracted crude oil often contains a certain amount of water. There are many ways to dehydrate crude oil, such as centrifugation, heating, electric dehydration and adding chemical demulsifiers. Due to its convenient operation and no need for special equipment, the crude oil dehydration method centered on chemical demulsification has been most widely used in the domestic petroleum industry. However, the use of chemical demulsifiers has many problems such as large dosage of demulsifiers, poor demulsification effect, poor adaptability, and serious environmental pollution. Therefore, from the perspective of reducing pollution and protecting the environment, it is necessary to find a cleaner demulsifier to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C10G33/00C12R1/01
Inventor 黄翔峰刘佳陆丽君闻岳徐竟成周琪李光明谢良林
Owner TONGJI UNIV
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