Low-temperature-resistant decoloring complex functional bacterial agent and preparation method thereof

A compound functional bacteria, low temperature-resistant technology, applied in biochemical equipment and methods, chemical instruments and methods, microorganism-based methods, etc., can solve the problem of increasing project investment and operating costs, sludge bulking, and difficult to guarantee sewage treatment effects, etc. problems, to achieve the effect of reducing costs, promoting emission standards, and reducing operating costs

Inactive Publication Date: 2019-08-23
新疆水处理工程技术研究中心有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Practice has proved that these measures not only increase project investment and operating costs, but also the effect of sewage treatment is difficult to guarantee, and often cause sludge bulking problems

Method used

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  • Low-temperature-resistant decoloring complex functional bacterial agent and preparation method thereof
  • Low-temperature-resistant decoloring complex functional bacterial agent and preparation method thereof

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preparation example Construction

[0035] The method steps of the preparation method of the low-temperature decolorization-resistant composite functional bacterial agent proposed by the present invention are as follows:

[0036] S1: Inoculate the purchased commercial Phanerochaete chrysosporium strains and Coriolus versicolor strains on sterilized potato dextrose agar medium respectively, and cultivate them at a constant temperature of 28°C for 5 days; the purchased commercial psychrophilic Bacillus strains, Depigmentation Bacillus strain, depigmentation Shewanella strain and Klebsiella strain were respectively inoculated on sterilized LB solid medium, and cultured at 20°C for 2 days;

[0037] S2: Pick Phanerochaete chrysosporium strain and Coriolus versicolor strain from potato dextrose agar medium and inoculate them in 100ml potato dextrose liquid medium, shake culture at 60r / min, 28℃ for 12h; from LB solid medium Pick Bacillus depigmentation, Shewanella depigmentation and Klebsiella strains from the above an...

Embodiment 1

[0041] A low-temperature decolorization-resistant composite functional bacterial agent proposed by the present invention comprises the following raw materials in weight percentage: 20% of Phanerochaete chrysosporium culture, 15% of psychrophilic Bacillus culture, and 15% of Decolorization Bacillus culture. %, Shewanella depigmentation culture 20%, Coriolus versicolor culture 15%, Klebsiella culture 14.95%, multivitamin 0.05%.

Embodiment 2

[0043] A low-temperature decolorization-resistant composite functional bacterial agent proposed by the present invention comprises the following raw materials in weight percentage: 21% of Phanerochaete chrysosporium culture, 16% of psychrophilic bacillus culture, 16% of decolorizing Bacillus culture %, Shewanella depigmentation culture 19%, Coriolus versicolor culture 14%, Klebsiella culture 13.94%, multivitamin 0.06%.

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Abstract

The invention discloses a low-temperature-resistant decoloring complex functional bacterial agent and a preparation method thereof. The low-temperature-resistant decoloring complex functional bacterial agent is prepared from, by weight, 20-25% of a phanerochaete chrysosporium culture, 15-20% of a psychrobacillus psychrodurans culture, 15-20% of a bacillus decolorationis culture, 15-20% of a shewanella decolorationis culture, 10-15% of a coriolus versicolor culture, 10-15% of a klebsiella pneumoniae culture and 0.05-0.08% of multi-vitamins. The complex bacterial agent contains various strains,the compatibility is reasonable, the occurrence rate of sludge bulking in winter can be reduced, dyes in printing and dyeing waste water can be efficiently decolorized and degraded at a low temperature, the input cost is low, and the low-temperature-resistant decoloring complex functional bacterial agent can be applied and popularized on a large scale.

Description

technical field [0001] The invention relates to the technical field of wastewater treatment, in particular to a low-temperature decolorization-resistant composite functional bacterial agent and a preparation method thereof. Background technique [0002] Printing and dyeing wastewater has high chroma, high concentration of organic matter, complex components, many refractory substances, and contains a large amount of inorganic salts, sulfides, etc. Treatment is supplemented by conventional physical and chemical methods, which are expensive, produce a lot of sludge, and are not widely used. Dye decolorization and degradation using microorganisms is considered to be a cost-effective method to remove these pollutants. [0003] Activated sludge is the most widely used method for sewage treatment. Temperature is one of the important factors affecting the treatment effect of activated sludge. During the treatment process, as the temperature changes, the characteristics of activated...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C02F3/34C12R1/07C12R1/22C12R1/01C12R1/645C02F101/30
CPCC12N1/20C12N1/14C02F3/341C02F3/347C02F2305/06C02F2101/308
Inventor 张锦玉张如来周镇江许白羽
Owner 新疆水处理工程技术研究中心有限公司
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