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Method for preparing prednisolone through bio-fermentation in one step

A technology of biological fermentation and prednisone, applied in fermentation and other directions, can solve the problems of adjustment and no process route.

Inactive Publication Date: 2017-06-27
TIANJIN JINYAO GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, researchers have made more efforts to innovate and improve the single-step process. However, due to traditional empirical thinking, the adjustment of the process route has not yet been carried out.

Method used

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  • Method for preparing prednisolone through bio-fermentation in one step
  • Method for preparing prednisolone through bio-fermentation in one step
  • Method for preparing prednisolone through bio-fermentation in one step

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Arthrobacter Simple ATCC 21032 was cultured in slant medium, primary culture and secondary culture in sequence, and the culture temperature was 29-32°C, and cortisone acetate dissolved in an organic solvent was put into the fermentation medium. In a 5L fermenter, the feed concentration is shown in the table below, and the reaction temperature is 31±1°C. The reaction time is shown in the table below. After the reaction is completed, the temperature is raised to 70°C to terminate the reaction, the fermentation broth is extracted with ethyl acetate, the organic phase is concentrated, and the conversion rate of prednisone is measured.

[0051]

Embodiment 2

[0053] The specific preparation method culture medium and feeding concentration are the same as in Example 1, except that Tween 80 is added to the 5L fermentation tank of the fermentation medium before cortisone acetate is put into use.

[0054]

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Abstract

The invention relates to a method for directly generating prednisolone through bio-fermentation in one step by using hydrocortisone acetate as a raw material and Arthrobacter Simple ATCC 21032.

Description

[0001] Technical field: [0002] The invention relates to a preparation method of prednisone, which is to prepare prednisone from cortisone acetate in one step by carrying out 1, 2-position dehydrogenation and 21-acetate hydrolysis reaction through biological fermentation. [0003] Background technique: [0004] Prednisone, a common corticosteroid, can be used not only as a corticosteroid treatment drug, but also as a raw material for other corticosteroid drugs. It has wide application value and is very important. Research on the preparation of this product continues at home and abroad. [0005] [0006] The commonly used starting material for domestic production of prednisone is mold oxide derived from diene. After ring-opening debromination, it is first oxidized at C11 and then dehydrogenated at C1 and 2, and then modified at the side chain to obtain 21 Bit esterification. The difficulty of this process lies in the introduction of C11-position carbonyl and C1, 2-position...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/02C12P33/00
CPCC12P33/02C12P33/005
Inventor 张杰王福军王延年
Owner TIANJIN JINYAO GRP
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