Bone tissue engineering scaffold material and preparation method thereof

A technology for bone tissue engineering and scaffold material, which is applied in the field of tissue engineering materials, bone tissue engineering scaffold materials and their preparation, can solve the problems of forming and degrading tissue engineering researchers, and achieves low cost of raw materials, mild and good reaction conditions. The effect of biocompatibility

Active Publication Date: 2015-10-21
CHONGQING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Some natural-synthetic materials overcome some problems, but problems

Method used

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  • Bone tissue engineering scaffold material and preparation method thereof
  • Bone tissue engineering scaffold material and preparation method thereof
  • Bone tissue engineering scaffold material and preparation method thereof

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preparation example Construction

[0038] The preparation method of bone tissue engineering scaffold material of the present invention, the specific steps are as follows,

[0039] 1) Preparation of various solutions required:

[0040] Dissolve silk fibroin powder in deionized water to make a silk fibroin solution with a concentration of 8-15mg / ml;

[0041] Dilute the phosphoric acid solution with deionized water to prepare a phosphoric acid solution with a concentration of 0.01-0.1mol / L;

[0042] Dissolve calcium hydroxide powder in deionized water to prepare a calcium hydroxide solution with a concentration of 0.01-0.05mol / L;

[0043] Dissolve sericin powder in deionized water to prepare a sericin solution with a concentration of 4%-8% (W / V);

[0044] Dissolving chitosan in a dilute acid solution to form a chitosan solution with a concentration of 2-3% (W / V); the dilute acid is dilute hydrochloric acid or dilute acetic acid with a concentration of 0.05-0.2mol / L;

[0045] Sodium glycerophosphate is dissolved...

Embodiment 1

[0062] A preparation method for bone tissue engineering scaffold material, specifically comprising the following steps:

[0063] (1) Dissolve sericin powder in deionized water to prepare a sericin solution with a concentration of 5.0% (W / V);

[0064] Chitosan is dissolved in the dilute hydrochloric acid solution that concentration is 0.1mol / L, is made into the chitosan solution that concentration is 2% (W / V);

[0065] Dissolve sodium glycerophosphate in deionized water to prepare a sodium glycerophosphate solution with a concentration of 50% (W / V);

[0066] (2) Add 0.05g silk fibroin / hydroxyapatite powder into the 1.34ml sericin solution obtained in step (1) at room temperature, and stir evenly;

[0067] (3) Add the sericin / (silk fibroin / hydroxyapatite) mixture obtained in step (2) into 10ml chitosan, stir and mix evenly; Add 2ml of the sodium glycerophosphate solution obtained in step (1) dropwise, stir for 30 minutes, put the mixed solution into a mold and adjust the pH to...

Embodiment 2

[0069] A preparation method for bone tissue engineering scaffold material, specifically comprising the following steps:

[0070] (1) Dissolve sericin powder in deionized water to prepare a sericin solution with a concentration of 5.0% (W / V);

[0071] Chitosan is dissolved in the dilute hydrochloric acid solution that concentration is 0.1mol / L, is made into the chitosan solution that concentration is 2% (W / V);

[0072] Dissolve sodium glycerophosphate in deionized water to prepare a sodium glycerophosphate solution with a concentration of 50% (W / V);

[0073] (2) Add 0.05g silk fibroin / hydroxyapatite powder into the 1ml sericin solution obtained in step (1) at room temperature, and stir evenly;

[0074] (3) Add the sericin / (silk fibroin / hydroxyapatite) mixture obtained in step (2) into 10ml chitosan, stir and mix evenly; at this time, adjust the temperature of the mixing system to 10°C, then Add 2ml of the sodium glycerophosphate solution obtained in step (1) dropwise, and sti...

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Abstract

The invention discloses a bone tissue engineering scaffold material and a preparation method thereof. The preparation method for the bone tissue engineering scaffold material comprises the steps that firstly, fibroin / hydroxyapatite powders are synthesized according to a bionic principle, the fibroin/ hydroxyapatite powders are dissolved in sericin solution, then the sericin solution (containing the fibroin/ hydroxyapatite powders), chitosan solution and sodium glycerophosphate solution are mixed, the mixed solution is turned into hydrogel by controlling the temperature, the hydrogel is dried through prefreezing, unfreezing, refreezing and final freezing, and the bone tissue engineering scaffold material is obtained. The bone tissue engineering scaffold material has the advantages that the mechanical property is good, the biocompatibility is good, the degradability and formability matching with osteocyte growing feature are good, and the osteocyte can be induced to grow into the material; the physiological process of the ingrowth of the osteocyte is accelerated, the performance to promote the growth of the osteocyte is achieved, the recovery time of a patient can be shortened, the clinic treatment process is accelerated, and the pain of the patient is decreased. The preparation method has the advantages of being mild in reaction condition, simple in technique, low in cost and suitable for amplifying production.

Description

technical field [0001] The invention relates to tissue engineering materials, in particular to a bone tissue engineering scaffold material and a preparation method thereof, belonging to the technical field of high-tech material manufacturing. Background technique [0002] The treatment of bone defects has always been one of the clinical problems in orthopedics. There are tens of millions of patients with fractures and bone defects worldwide every year. The traditional treatment methods mainly include autologous bone transplantation, allogeneic bone transplantation, and artificial bone transplantation. However, these methods have major defects. As the "gold standard" of bone transplantation technology, autologous bone transplantation has defects such as new lesions, limited sources, bone area infection, pain, and long operation time; Severe immune rejection, etc.; artificial bone grafts have defects such as limited sources, difficulty in osteogenesis, and large variation i...

Claims

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Application Information

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IPC IPC(8): A61L27/26A61L27/22A61L27/20A61L27/12A61L27/56
Inventor 陈忠敏李江峰王富平庞亚妮胡奇
Owner CHONGQING UNIV OF TECH
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