A kind of bone tissue engineering scaffold material and preparation method thereof

A technology for bone tissue engineering and scaffold materials, applied in the field of tissue engineering materials, bone tissue engineering scaffold materials and their preparation, can solve the problems of forming and degrading tissue engineering researchers, and achieve low cost of raw materials, suitable for large-scale production, The effect of mild reaction conditions

Active Publication Date: 2017-10-20
CHONGQING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some natural-synthetic materials overcome some problems, but problems in molding and degradation also plague tissue engineering researchers

Method used

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  • A kind of bone tissue engineering scaffold material and preparation method thereof
  • A kind of bone tissue engineering scaffold material and preparation method thereof
  • A kind of bone tissue engineering scaffold material and preparation method thereof

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preparation example Construction

[0038] The preparation method of bone tissue engineering scaffold material of the present invention, the specific steps are as follows,

[0039] 1) Preparation of various solutions required:

[0040] Dissolve silk fibroin powder in deionized water to make a silk fibroin solution with a concentration of 8-15mg / ml;

[0041] Dilute the phosphoric acid solution with deionized water to prepare a phosphoric acid solution with a concentration of 0.01-0.1mol / L;

[0042] Dissolve calcium hydroxide powder in deionized water to prepare a calcium hydroxide solution with a concentration of 0.01-0.05mol / L;

[0043] Dissolve sericin powder in deionized water to prepare a sericin solution with a concentration of 4%-8% (W / V);

[0044] Dissolving chitosan in a dilute acid solution to form a chitosan solution with a concentration of 2-3% (W / V); the dilute acid is dilute hydrochloric acid or dilute acetic acid with a concentration of 0.05-0.2mol / L;

[0045] Sodium glycerophosphate is dissolved...

Embodiment 1

[0062] A preparation method for bone tissue engineering scaffold material, specifically comprising the following steps:

[0063] (1) Dissolve sericin powder in deionized water to prepare a sericin solution with a concentration of 5.0% (W / V);

[0064] Chitosan is dissolved in the dilute hydrochloric acid solution that concentration is 0.1mol / L, is made into the chitosan solution that concentration is 2% (W / V);

[0065] Dissolve sodium glycerophosphate in deionized water to prepare a sodium glycerophosphate solution with a concentration of 50% (W / V);

[0066] (2) Add 0.05g silk fibroin / hydroxyapatite powder into the 1.34ml sericin solution obtained in step (1) at room temperature, and stir evenly;

[0067] (3) Add the sericin / (silk fibroin / hydroxyapatite) mixture obtained in step (2) into 10ml chitosan, stir and mix evenly; Add 2ml of the sodium glycerophosphate solution obtained in step (1) dropwise, stir for 30 minutes, put the mixed solution into a mold and adjust the pH to...

Embodiment 2

[0069] A preparation method for bone tissue engineering scaffold material, specifically comprising the following steps:

[0070] (1) Dissolve sericin powder in deionized water to prepare a sericin solution with a concentration of 5.0% (W / V);

[0071] Chitosan is dissolved in the dilute hydrochloric acid solution that concentration is 0.1mol / L, is made into the chitosan solution that concentration is 2% (W / V);

[0072] Dissolve sodium glycerophosphate in deionized water to prepare a sodium glycerophosphate solution with a concentration of 50% (W / V);

[0073] (2) Add 0.05g silk fibroin / hydroxyapatite powder into the 1ml sericin solution obtained in step (1) at room temperature, and stir evenly;

[0074] (3) Add the sericin / (silk fibroin / hydroxyapatite) mixture obtained in step (2) into 10ml chitosan, stir and mix evenly; at this time, adjust the temperature of the mixing system to 10°C, then Add 2ml of the sodium glycerophosphate solution obtained in step (1) dropwise, and sti...

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Abstract

The invention discloses a scaffold material for bone tissue engineering and a preparation method thereof. First, silk fibroin / hydroxyapatite powder is synthesized according to the bionic principle, dissolved in a sericin solution, and then the sericin solution (containing silk fibroin / hydroxyl Apatite), chitosan solution, and sodium glycerophosphate solution are mixed, and then the mixed solution is converted into a gel by temperature control, and the gel is pre-frozen, thawed, re-frozen, and finally freeze-dried to obtain bone tissue Engineering support material. The tissue engineering scaffold material has the advantages of excellent mechanical properties, good biocompatibility, degradability matching bone cell growth, good formability, and the ability to induce bone cells to grow into the material. The scaffold material can accelerate the physiological process of bone cell ingrowth, has the property of promoting bone growth, can shorten the recovery time of patients, accelerate the process of clinical treatment, and reduce the pain of patients. The preparation method has the advantages of mild reaction conditions, simple process, low cost and suitable for enlarged production.

Description

technical field [0001] The invention relates to tissue engineering materials, in particular to a bone tissue engineering scaffold material and a preparation method thereof, belonging to the technical field of high-tech material manufacturing. Background technique [0002] The treatment of bone defects has always been one of the clinical problems in orthopedics. There are tens of millions of patients with fractures and bone defects worldwide every year. The traditional treatment methods mainly include autologous bone transplantation, allogeneic bone transplantation, and artificial bone transplantation. However, these methods have major defects. As the "gold standard" of bone transplantation technology, autologous bone transplantation has defects such as new lesions, limited sources, bone area infection, pain, and long operation time; Severe immune rejection, etc.; artificial bone grafts have defects such as limited sources, difficulty in osteogenesis, and large variation in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/26A61L27/22A61L27/20A61L27/12A61L27/56
Inventor 陈忠敏李江峰王富平庞亚妮胡奇
Owner CHONGQING UNIV OF TECH
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