Composite degradable antibacterial artificial cerebral dura mater and preparation method thereof

An artificial dura mater and composite technology, applied in medical science, prosthesis, etc., can solve problems such as incomplete satisfaction, and achieve the effect of good film quality, good biological characteristics, and good compatibility

Inactive Publication Date: 2014-03-26
BEIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also many patients with cerebrospinal fluid leakage, none of which can fully meet the above requirements

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Taking bovine tendon as one of the decellularized membranous biologically derived materials as an example to illustrate its specific composite degradable antibacterial artificial dura mater preparation method, the other decellularized membranous biologically derived materials are the same as in Example 1 : Include the following steps:

[0021]1) Preparation of sponge-like collagen biofilm scaffolds; prepared by freeze-drying collagen membranes, with a porous and loose structure, and good penetration and water absorption capabilities. Bovine tendon, one of the decellularized membrane-like bio-derived materials, was placed at 75 % ethanol solution and peracetic acid for 2 hours, rinsed repeatedly with phosphate buffer for 3 times; soaked in sodium hydroxide solution for 2 hours, then rinsed with phosphate buffer until pH7.4; Freeze in low-temperature liquid nitrogen at -80°C for 24 hours, freeze-dry in a vacuum freeze dryer for 24 hours, and pulverize with a li...

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PUM

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Abstract

The invention provides a composite degradable antibacterial artificial cerebral dura mater, comprising a vancomycin or ofloxacin hydrochloride injection, chitosan and sodium beta-glycerophosphate. The invention is characterized in that a spongy collagen biomembrane stent is prepared from a decellularized membrane-like derivative material, a chitosan solution with a concentration of 2% (w / v) and a sodium beta-glycerophosphate solution with a concentration of 56% (w / v) are used and mixed, and since a hydrogel formed by a compound of the chitosan solution and the sodium beta-glycerophosphate solution at a temperature of 37 DEG C has the characteristic of temperature sensitivity, the vancomycin or ofloxacin hydrochloride injection is added into the compound to form a solution which is used for soaking of the prepared spongy collagen biomembrane stent to prepare a suture-free cerebral dura mater, and the hydrogel compound uniformly infiltrates into the spongy collagen biomembrane stent after disposition in an incubator with a temperature of 37 DEG C for 10 min so as to form the absorbable artificial cerebral dura mater with antibacterial activity. The invention further provides a preparation method. The preparation method has the characteristics of convenience in preparation, easy film formation, low cost, convenient operation, etc.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and belongs to the biomaterials available to the public, in particular to a composite degradable antibacterial artificial dura mater and a preparation method thereof. Background technique [0002] Chitosan, English chitosan, is a copolymer of p(1-4)-2-acetylamino-2-deoxy-D dextran and 2-amino-2-deoxy-D dextran. Biodimers are mainly obtained by deacetylating chitin. Due to the wide range of sources, non-toxicity, good tissue compatibility, biodegradability and adhesion, the material has been deeply studied and widely used in the fields of medicine and biology. Chenite et al. (See CheniteA, ChaputC, WangD, et al. Novel injectable neutral solutions of chitosan form biodegradable gels in situ[J]. Biomaterials, 2000,21(21):2155-2161.) Neutralize chitosan with sodium glycerophosphate Finally, chitosan / β, which has a neutral pH value and can remain liquid for a long time at room temperature, is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/24A61L27/20A61L27/54
Inventor 宁显宾张爱荣何巍赵长福逄锦峰丁照义
Owner BEIHUA UNIV
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