Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Recombinant collagen and thermo-sensitive hydrogel thereof

A technology for recombining collagen and temperature-sensitive hydrogel, which is applied in the field of peptide technology and biomedical materials, can solve the problems of weak mechanical strength and no research on the influence of collagen, and achieve the effect of easy degradation and no cytotoxicity

Active Publication Date: 2018-01-05
南京艾澜德生物科技有限公司
View PDF7 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are also researches on this in the prior art. For example, Chinese patent application CN103656749A discloses a composite degradable antibacterial artificial dura mater and its preparation method, which includes vancomycin or ofloxacin hydrochloride injection, chitosan and Sodium glycerophosphate, however collagen is not addressed in this patent and its effect is not studied
[0004] Chinese patent application CN104971352A discloses an ophthalmic chitosan drug-loaded thermosensitive hydrogel and its preparation method, wherein polyvinyl alcohol is required and the mechanical strength is very weak

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Recombinant collagen and thermo-sensitive hydrogel thereof
  • Recombinant collagen and thermo-sensitive hydrogel thereof
  • Recombinant collagen and thermo-sensitive hydrogel thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1 Preparation of recombinant collagen of the present invention

[0052] According to the recombinant collagen protein designed by the inventor (the amino acid sequence is shown in SEQ ID NO: 1), Shanghai Sangon Bioengineering Co., Ltd. was commissioned to synthesize its coding gene and clone it into the pPIC9K vector, and electrotransform it into the Pichiapastoris yeast expression strain GS115. After the positive transformants were cultured in BMGY medium for 24 hours, they were inoculated in fermentation medium (recipe: glycerol 40g, K 2 SO 4 18.2g, H 3 PO 4 26.7ml, CaSO 4 .2H 2 O0.93g, MgSO 4 14.9g, KOH 4.13g, dilute to 1L with water) culture at 29°C, control dissolved oxygen at 22-25%, after 4 hours of culture, add methanol to a final concentration of 0.5% (V / V) to induce expression, at 29 Continue shaking culture at ℃ for 72 hours, during which 100% methanol is supplemented every 24 hours to keep the final concentration of methanol at 0.5% (V / V...

Embodiment 2

[0054] Embodiment 2 Preparation of thermosensitive hydrogel of the present invention 1

[0055]Chitosan (Beijing Bailingwei Technology Co., Ltd., molecular weight distribution 100-300kDa) was dissolved in 0.1M acetic acid solution, prepared into a solution with a mass concentration of 2%, added to a dialysis bag for dialysis for 3 days, and then freeze-dried. Freeze-dried chitosan and recombinant collagen were mixed and dissolved in deionized water, wherein the mass concentrations of chitosan and collagen were 1.5% and 2.5%, respectively, and then carbodiimide (EDC) and N-hydroxysuccinate were added Imide (NHS) was mixed to a final concentration of 5 μM and 15 μM, respectively, and then left to stand at room temperature for 1 hour, dialyzed at room temperature, and freeze-dried. The lyophilized reactant was dissolved in deionized water to prepare a solution with a mass concentration of 2%, and then 100 microliters of a solution containing 50% (W / W) β-glycerophosphate and 0.4M ...

Embodiment 3

[0056] Embodiment 3 Preparation of thermosensitive hydrogel of the present invention 2

[0057] Chitosan (Beijing Bailingwei Technology Co., Ltd., molecular weight distribution 100-300kDa) was dissolved in 0.15M acetic acid solution to prepare a solution with a mass concentration of 3%, added to a dialysis bag for dialysis for 5 days, and then freeze-dried. Freeze-dried chitosan and recombinant collagen were mixed and dissolved in deionized water, wherein the mass concentrations of chitosan and collagen were 2% and 3%, respectively, and then EDC and NHS were added to final concentrations of 10 μM and 30 μM, respectively, After mixing evenly, let stand at room temperature for 2 hours, dialyze at room temperature and freeze-dry. The freeze-dried reactant was dissolved in deionized water to prepare a solution with a mass concentration of 3%, and then 100 microliters of a solution containing 50% (W / W) β-glycerophosphate and 0.4M sodium bicarbonate were added to every milliliter of...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
phase transition temperatureaaaaaaaaaa
mechanical strengthaaaaaaaaaa
Login to View More

Abstract

The invention provides recombinant collagen and thermo-sensitive hydrogel of the recombinant collagen. The invention further provides a preparation method of the thermo-sensitive hydrogel, comprisingthe following step: standing chitosan, collagen, carbodiimide, N-hydroxysuccinimide, beta-sodium glycerophosphate and a sodium bicarbonate solution at the temperature of 37 DEG C.

Description

technical field [0001] The invention belongs to the fields of polypeptide technology and biomedical materials, in particular, the invention relates to recombinant collagen and its temperature-sensitive hydrogel. Background technique [0002] Thermosensitive hydrogels are smart materials that can sense changes in the external environment temperature and transform into gels. Utilizing its property that it is a flowing liquid at low temperature and transforms into a gel when it reaches a certain temperature, by adjusting the phase transition temperature to be close to body temperature, an injectable temperature-sensitive hydrogel for human body can be obtained. Such hydrogels are easy to mix with drugs or cells at low temperature, and have a certain mechanical strength after being gelled. In recent years, they have broad application prospects in drug sustained release and tissue repair. [0003] There are also researches on this in the prior art. For example, Chinese patent ap...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C12N15/12C12P21/02C08J9/40C08J9/28C08J3/075A61L27/20A61L27/24A61L27/52
Inventor 杨树林康肸邓爱鹏
Owner 南京艾澜德生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com