Preparation method of in-situ gel based on magnetic graphene oxide and application thereof

An in-situ gel and oxide stone technology, applied in the field of medicine, can solve problems such as applications that have not been reported, and achieve the effect of abundant sources, low cost, and good quality

Inactive Publication Date: 2015-07-08
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, a high-molecular water-soluble polymer-polyethyleneimine is grafted on the surface of magnetic graphene oxide, and prepared into an in-situ gel, and it is used as a magnetic hyperthermia medium and drug delivery carrier in the preparation of tumor drugs The application has not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The inventive method is realized by the following steps in concrete implementation:

[0018] 1) Preparation of magnetic graphene oxide: Weigh 20 mg of graphene oxide and dissolve it in 20 ml of a mixed solution of ethylene glycol and diethylene glycol, stir evenly, the volume ratio of ethylene glycol to diethylene glycol is 1:19, and then sonicate 30min, then under magnetic stirring, add 300mg of sodium acetate and 108mg of ferric chloride hexahydrate in sequence to form a uniform yellow suspension. After 5 minutes, the precipitate was obtained. After the precipitate was centrifuged and washed 20 times with 200ml of absolute ethanol and 300ml of ultrapure water, the precipitate was vacuum-dried for 12 hours to obtain magnetic graphene oxide;

[0019] 2) Preparation of polyethyleneimine-modified magnetic graphene oxide: Weigh 30 mg of magnetic graphene oxide and dissolve in 30 ml of phosphate buffer at pH 7.4, stir, and ultrasonically 30 min to disperse evenly; add 1-eth...

Embodiment 2

[0024] The inventive method is realized by the following steps in concrete implementation:

[0025] 1) Preparation of magnetic graphene oxide: Weigh 30 mg of graphene oxide and dissolve it in 30 ml of a mixed solution of ethylene glycol and diethylene glycol, stir evenly, the volume ratio of ethylene glycol to diethylene glycol is 1:19, and then sonicate 30min, then under magnetic stirring, add sodium acetate 450mg, ferric chloride hexahydrate 300mg in turn to form a uniform yellow suspension, put the suspension in the reaction kettle, react at 200°C for 10h, and then centrifuge the suspension at 3000r / min After 5 minutes, the precipitate was obtained. After the precipitate was centrifuged and washed 20 times with 300ml of absolute ethanol and 450ml of ultrapure water, the precipitate was vacuum-dried for 12 hours to obtain magnetic graphene oxide;

[0026] 2) Preparation of polyethyleneimine-modified magnetic graphene oxide: Weigh 40 mg of magnetic graphene oxide and dissolve...

Embodiment 3

[0031] The inventive method is realized by the following steps in concrete implementation:

[0032] 1) Preparation of magnetic graphene oxide: Weigh 40 mg of graphene oxide and dissolve in 40 ml of ethylene glycol and diethylene glycol mixed solution, stir evenly, the volume ratio of ethylene glycol and diethylene glycol is 1:19, and then ultrasonic 30min, then under magnetic stirring, sequentially add 400mg of sodium acetate and 300mg of ferric chloride hexahydrate to form a uniform yellow suspension. After 5 minutes, the precipitate was obtained. After the precipitate was centrifuged and washed 20 times with 200ml of absolute ethanol and 300ml of ultrapure water, the precipitate was vacuum-dried for 24 hours to obtain magnetic graphene oxide;

[0033] 2) Preparation of polyethyleneimine-modified magnetic graphene oxide (GO-IONP-PEI): Weigh 30 mg of magnetic graphene oxide and dissolve it in 30 ml of phosphate buffer solution at pH 7.4, stir, and ultrasonicate for 30 minutes ...

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Abstract

The invention relates to a preparation method of in-situ gel based on magnetic graphene oxide and application thereof and effectively solves a problem of the application of the magnetic graphene oxide in-situ gel as a magnetic thermal-therapy medium and a drug transport carrier in treatment of tumors. In the preparation method, the in-situ gel based on the magnetic graphene oxide, as a drug carrier system, is prepared by mixing the magnetic graphene oxide on which a drug is carried with a chitosan / [beta]-sodium glycerophosphate gel, wherein the volume ratio of the magnetic graphene oxide on which the drug is carried to the chitosan / [beta]-sodium glycerophosphate gel is 1-3:1. On the originally basis that the graphene oxide supports an iron oxide, by means of a condensation reaction, a macromolecular polymer, polyethyleneimine, which is strong in reaction activity, is connected to the surface of the graphene oxide to generate a water-soluble magnetic carrier, and finally the carrier is mixed with the chitosan in-situ gel to prepare the magnetic graphene oxide in-situ gel. The raw materials are wide in sources. The in-situ gel based on the magnetic graphene oxide is low in cost, is good in quality, is strong in targeting, is good in use effect and is a great creative achievement on tumor treatment drugs.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a preparation method and application of magnetic graphene oxide-based in-situ gel. Background technique [0002] Graphene oxide (Graphene oxide, GO) is a single layer of graphite oxide, because it has a large number of sp 2 Carbon atoms have a large specific surface area, so chemical drugs, DNA, RNA, etc. can be effectively immobilized on graphene oxide through non-covalent bonding such as π-π conjugation and electrostatic interaction. GO has good biocompatibility and transmembrane ability, so it is widely used as a drug carrier in the field of medicine. However, it coagulates rapidly in normal saline and phosphate buffer, so that it cannot be directly applied in the field of medicine and biology. It is known that functional modification of it can reduce toxicity, so the surface modification of graphene oxide is very necessary. Polyethylene imine (PEI) is a water-soluble polymer materia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/06A61K47/46A61K47/32A61K47/04A61K31/704A61P35/00
Inventor 祝侠丽黄河清张英杰李志史进进张振中
Owner ZHENGZHOU UNIV
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