Placenta-derived mesenchymal stem cell freezing medium and freezing method thereof

A technology of mesenchymal stem cells and cryopreservation method, which is applied in the field of placental mesenchymal stem cell cryopreservation solution, which can solve problems such as protein denaturation, affecting the survival rate of stem cells, and stem cell damage

Inactive Publication Date: 2017-09-22
魏方萌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, stem cells are mainly preserved by cryopreservation, but the process of cryopreservation will cause damage to stem cells, so it is necessary to add cryopreservation solution to slow down cell damage
The existing cryopreservation solution components include dimethyl sulfoxide, albumin and 1640 medium rich in various nutrients. As a osm

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A cryopreservation solution for embryonic mesenchymal stem cells, comprising the following components in parts by weight: 45 parts of DEME medium, 3 parts of sodium glycerophosphate solution (mass concentration 0.5g / L), 2 parts of hydroxyethyl starch, manna 2 parts of alcohol solution (mass concentration 0.5g / L), 0.3 part of ethylene glycol, 0.5 part of polyvinyl alcohol, 0.5 part of trehalose and 0.5 part of polyvinylpyrrolidone.

Embodiment 2

[0029] A cryopreservation solution for embryonic mesenchymal stem cells, comprising the following components in parts by weight: 55 parts of DEME medium, 4 parts of sodium glycerophosphate solution (mass concentration 1g / L), 3 parts of hydroxyethyl starch, mannitol 3 parts of solution (mass concentration 0.8g / L), 0.5 part of ethylene glycol, 1 part of polyvinyl alcohol, 0.8 part of trehalose, 2 parts of collagen and 0.8 part of polyvinylpyrrolidone.

Embodiment 3

[0031] A cryopreservation solution for embryonic mesenchymal stem cells, 68 parts of DEME medium, 6 parts of sodium glycerophosphate solution (mass concentration 1.5g / L), 5 parts of hydroxyethyl starch, 4 parts of mannitol solution (mass concentration 1.2g / L) L), 0.8 parts of ethylene glycol, 2 parts of polyvinyl alcohol, 1.5 parts of trehalose, 2.5 parts of collagen and 1.5 parts of polyvinylpyrrolidone.

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Abstract

The invention relates to the technical field of stem cells, in particular to a placenta-derived mesenchymal stem cell freezing medium. The placenta-derived mesenchymal stem cell freezing medium comprises the following components in parts by weight: 40-70 parts of DEME culture medium, 2-6 parts of sodium glycerophosphate solution, 1-5 parts of hydroxyethyl starch, 1-4 parts of mannitol solution, 0.3-0.8 part of ethanediol, 0.5-2 parts of polyvinyl alcohol, 0.5-1.8 parts of trehalose, and 0.5-1.5 parts of polyvinylpyrrolidone. With the adoption of the freezing medium for storing placenta-derived mesenchymal stem cells, the damage on the stem cells in the freezing process can be reduced, and the survival rate of the stem cells after thawing is increased.

Description

technical field [0001] The invention relates to the technical field of stem cells, in particular to a cryopreservation solution for placental mesenchymal stem cells and a cryopreservation method thereof. Background technique [0002] Stem cells are a type of pluripotent cells with self-replicating ability. Under certain conditions, it can differentiate into a variety of functional cells. Stem cells are divided into embryonic stem cells (ES cells) and adult stem cells (somatic stem cells) according to their developmental stages. Stem cells are divided into three categories according to their developmental potential: totipotent stem cells (TSCs), pluripotent stem cells (pluripotent stem cells) and unipotent stem cells (unipotent stem cells). Stem cells are not fully differentiated and immature cells, which have the potential to regenerate various tissues and organs and the human body, and are called "universal cells" in the medical field. [0003] The placenta contains a va...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0284
Inventor 魏方萌
Owner 魏方萌
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