Placenta-derived mesenchymal stem cell freezing medium and freezing method thereof

Abstract

The invention relates to the technical field of stem cells, in particular to a placenta-derived mesenchymal stem cell freezing medium. The placenta-derived mesenchymal stem cell freezing medium comprises the following components in parts by weight: 40-70 parts of DEME culture medium, 2-6 parts of sodium glycerophosphate solution, 1-5 parts of hydroxyethyl starch, 1-4 parts of mannitol solution, 0.3-0.8 part of ethanediol, 0.5-2 parts of polyvinyl alcohol, 0.5-1.8 parts of trehalose, and 0.5-1.5 parts of polyvinylpyrrolidone. With the adoption of the freezing medium for storing placenta-derived mesenchymal stem cells, the damage on the stem cells in the freezing process can be reduced, and the survival rate of the stem cells after thawing is increased.

Description

technical field [0001] The invention relates to the technical field of stem cells, in particular to a cryopreservation solution for placental mesenchymal stem cells and a cryopreservation method thereof. Background technique [0002] Stem cells are a type of pluripotent cells with self-replicating ability. Under certain conditions, it can differentiate into a variety of functional cells. Stem cells are divided into embryonic stem cells (ES cells) and adult stem cells (somatic stem cells) according to their developmental stages. Stem cells are divided into three categories according to their developmental potential: totipotent stem cells (TSCs), pluripotent stem cells (pluripotent stem cells) and unipotent stem cells (unipotent stem cells). Stem cells are not fully differentiated and immature cells, which have the potential to regenerate various tissues and organs and the human body, and are called "universal cells" in the medical field. [0003] The placenta contains a va...

AI Technical Summary

Problems solved by technology

At present, stem cells are mainly preserved by cryopreservation, but the process of cryopreservation will cause damage to stem cells, so it is necessary to add cryopreservation solution to slow down cell damage

The existing cryopreservation solution components include dimethyl sulfoxide, albumin and 1640 medium rich in various nutrients. As a osmotic protectant, dimethyl sulfoxide has strong penetration ability It can lower the freezing point of cells and reduce the damage to cells during freezing, but dimethyl sulfoxide is toxic and has a large proportion in the cryopreservation solution, which can easily cause protein denaturation and ultimately affect the survival rate of stem cells