Thiolated-chitosan-based temperature-sensitive in-situ hydrogel as well as preparation method and uses thereof

A technology of thiolated chitosan and chitosan group is applied in the field of polymer hydrogel, which can solve the problems of long gel time, limited applicability, limited application and the like, and achieve the effect of simple and convenient operation.

Inactive Publication Date: 2011-11-16
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the formation of the above hydrogel, the phase transition time of the gel ranges from tens of minutes to several hours, and the gel time is longer, which limits its application in some fields.
Sakloetsakun D etc. shortened the gel transition time to a few minutes by adding an oxidant to an aqueous solution of thiolated chitosan (Duangkamon Sakloetsakun, et al.Biomaterial, 2009, 30(31), 6151-6157), but due to (NH 4 ) 2 S 2 o 8 ) form free radicals and its strong oxidative properties will introduce certain cytotoxicity, so its applicability will be limited

Method used

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  • Thiolated-chitosan-based temperature-sensitive in-situ hydrogel as well as preparation method and uses thereof
  • Thiolated-chitosan-based temperature-sensitive in-situ hydrogel as well as preparation method and uses thereof
  • Thiolated-chitosan-based temperature-sensitive in-situ hydrogel as well as preparation method and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1.08g of β-glycerophosphate sodium powder (B) was dissolved in 10mL of double distilled water to prepare a 10.8% β-glycerophosphate sodium aqueous solution, and 40mg of chitosan had a number-average molecular mass of 50kDa and a degree of mercaptolation of 250 μmol / g Thioglycolic acid modified chitosan (A) and 5 μmol polyethylene glycol diacrylate (C) with a number-average molecular weight of 0.258 kDa were dissolved in 960 mg of β-glycerophosphate sodium aqueous solution to make mercapto-chitosan The A-B-C ternary aqueous solution with a sugar concentration of 4% (w / w) forms an A-B-C ternary temperature-sensitive in-situ hydrogel at 34-36°C, which is labeled as "Gel 1".

Embodiment 2

[0023] The device and operation are the same as in Example 1. Dissolve 0.216g of β-sodium glycerophosphate powder in 10mL of double-distilled water to prepare a 2% aqueous solution of β-glycerophosphate sodium. Thioglycolic acid-modified chitosan with a concentration of 250 μmol / g and polyethylene glycol diacrylate with a number-average relative molecular mass of 2.5 μmol of 0.7 kDa were dissolved in 960 mg of β-glycerophosphate sodium aqueous solution to make mercapto-chitosan The A-B-C aqueous solution with a sugar concentration of 4% (w / w) forms an A-B-C ternary temperature-sensitive in-situ hydrogel at 35-37°C, which is marked as "Gel 2".

Embodiment 3

[0025] The device and operation are the same as in Example 1. Dissolve 1.08g of β-sodium glycerophosphate powder in 10mL of double-distilled water to prepare a 10% aqueous solution of β-glycerophosphate sodium. Thioglycolic acid-modified chitosan with a concentration of 250 μmol / g and polyethylene glycol diacrylate with a number-average molecular mass of 2.0 kDa of 2.5 μmol were dissolved in 960 mg of β-glycerophosphate sodium aqueous solution to prepare mercapto-chitosan The A-B-C aqueous solution with a sugar concentration of 4% (w / w) forms an A-B-C ternary temperature-sensitive in-situ hydrogel at 23-24°C, which is labeled as "Gel 3".

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Abstract

The invention discloses a thiolated-chitosan-based temperature-sensitive in-situ hydrogel as well as a preparation method and uses thereof. The thiolated-chitosan-based temperature-sensitive in-situ hydrogel is tribasic or dibasic temperature-sensitive in-situ hydrogel consisting of 0.5 to 10% of thiolated chitosan by mass, 1 to 30% of sodium glycero-phosphate by mass, (methyl) acrylic ester-based polymer of which the use amount is 0 to 0.5 time as much as the molar weight of sulfhydryl in the thiolated chitosan, and the balance of water; the preparation method of the thiolated-chitosan-based temperature-sensitive in-situ hydrogel comprises the steps of preparing an aqueous solution from the thiolated chitosan, the sodium glycero-phosphate and the (methyl) acrylic ester-based polymer, stirring uniformly and forming the hydrogel in situ at 10 DEG C to 50 DEG C; and the thiolated-chitosan-based temperature-sensitive in-situ hydrogel has water-absorbability, permeability, excellent biocompatibility and biodegradability and higher mechanical strength, is simple and convenient to operate, and can be widely used in biomedicines and other aspects.

Description

technical field [0001] The invention relates to a thiol-based chitosan-based temperature-sensitive in-situ hydrogel and a preparation method and application thereof. The invention belongs to the technical field of polymer hydrogel. Background technique [0002] Hydrogel is a kind of hydrophilic network polymer swelling body that can swell in water, absorb and retain a large amount of water without dissolving in water. The hydrogel has a smooth surface and excellent biocompatibility. Compared with hydrophobic polymers, hydrophilic hydrogels have very weak interactions with immobilized enzymes or cells, and the activity of biomolecules immobilized in hydrogels can be maintained for a long time. Since hydrogels can provide an aqueous environment similar to intercellular matrix, they are considered to be more suitable cell scaffold materials. In situ gel refers to a non-chemically cross-linked semi-solid preparation that undergoes a phase transition at the site of administrat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L5/08C08L71/08C08K5/053C08J3/075C12N5/00C12N11/10C12N11/08A61K47/36A61K47/34
Inventor 邓联东董岸杰陈晨
Owner TIANJIN UNIV
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