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Injectable chitosan composite hydrogel capable of promoting myocardium repair and preparation method of injectable chitosan composite hydrogel

A technology of composite hydrogel and chitosan, which is applied in medical science, prosthesis, etc., can solve the problems of poor biocompatibility and poor mechanical properties of injectable hydrogel, and achieve good biocompatibility, softness and hardness. Controllable and repeatable results

Active Publication Date: 2015-09-23
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Purpose of the invention: In order to overcome the deficiencies in the prior art, the present invention provides an injectable chitosan composite hydrogel for myocardial repair and its preparation method, which can inject materials into cardiac defects or infarctions by injection tissue and quickly form a three-dimensional scaffold in situ that is complementary to the shape of the defect, and can promote the growth of cardiomyocytes in the damaged area to achieve the purpose of repair; this product can solve the problem of poor biocompatibility and poor mechanical properties of current injectable hydrogels technical issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Extraction of human hair keratin

[0044] a) Weigh 2g of human hair test sample (purchased from a barber shop), cut into fragments of about 2cm, and place them in a 400mL Erlenmeyer flask;

[0045] b) Add 100 mL of peracetic acid with a mass concentration of 2%, and shake at 150 rpm on a shaker at 37°C for 24 hours;

[0046] c) Filter through a sieve with a pore size of 350 μm, rinse the hair test sample with ultrapure water, and remove excess oxidant;

[0047] d) Treat with 200mL of 100mM Tris solution on a shaker at 37°C at 150rpm for 2h, filter through a 350μm sieve, and retain the filtrate, continue processing with 200mL of ultrapure water for 1.5h, filter through a 350μm sieve, and retain the filtrate;

[0048] e) The two filtrates are combined, centrifuged at 6000 rpm for 10 min to remove insoluble matter, and then filtered with filter paper;

[0049] f) Dialyze with a dialysis bag (molecular weight cut-off 3K) for 24 hours, concentrate the solution, and fre...

Embodiment 2

[0060] The difference between this example and Example 1 is: take 0.1mL sodium β-glycerophosphate solution, add 10 μL keratin solution, mix well, add into 1mL chitosan solution, stir evenly until the solution is clear without turbidity. Add 0.3 mL of genipin solution, stir evenly, and let stand to obtain chitosan / keratin composite hydrogel. Others are the same as in Example 1. The gel time of this embodiment is 2min.

Embodiment 3

[0062] The difference between this example and Example 1 is: take 0.1mL sodium β-glycerophosphate solution, add 20 μL keratin solution, mix well, add 1mL chitosan solution, stir evenly until the solution is clear without turbidity. Add 0.1 mL of genipin solution, stir evenly, and let stand to obtain chitosan / keratin composite hydrogel. Others are the same as in Example 1. The gel time of this embodiment is 6min.

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Abstract

The invention discloses injectable chitosan / keratin composite hydrogel capable of promoting myocardium repair and a preparation method of the hydrogel. The hydrogel consists of four solutions, namely chitosan, sodium beta-glycerophosphate, genipin and keratin according to a volume ratio of 10: (1-5): (1-3): (0.1-0.5). The preparation method comprises the following steps: 1) extracting keratin from human fairs by using peracetic acid and Tris alkaline; 2) adjusting the pH value of a water-soluble chitosan solution by using sodium beta-glycerophosphate so as to be close to a microenvironment in a human body; 3) introducing genipin as a crosslinking agent, and controlling the gelling time of a hydrogel solution by changing the use amount of the genipin so as to achieve the purpose that the mixed solution can be rapidly gelled in situ after being injected into an organism. Experiments prove that the composite hydrogel has favorable biocompatibility and has the capability of remarkably promoting the growth of myocardial cells; the hydrogel is wide in sources of materials and low in cost and has a broad application prospect in the field of tissue engineering repair.

Description

technical field [0001] The invention relates to an injectable chitosan composite hydrogel for promoting myocardial repair and a preparation method thereof, belonging to the field of tissue engineering. Background technique [0002] A hydrogel is a polymeric network structure with hydrophilic groups or regions in the polymer, which can be hydrated under certain conditions to produce a gel structure. Hydrogels can play multiple roles in the field of tissue engineering. For example, as a packing hemostatic material, a carrier of bioactive molecules, and as a three-dimensional scaffold to load cells and guide tissue morphogenesis and remodeling. However, the mechanical properties of hydrogels are generally weak, it is difficult to sterilize, and it is difficult to carry out in vitro cross-linking after loading drugs and cells without affecting their activity. [0003] Injectable hydrogels capable of forming scaffolds in situ are relatively low-viscosity aqueous solutions befor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/52A61L27/22A61L27/20A61L27/54A61L27/50
Inventor 黄宁平张峰孟泓旭
Owner SOUTHEAST UNIV
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