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Method for preparing injectable chitosan/sodium glycerophosphate/collagen hydrogel

A technology of sodium glycerophosphate and collagen hydrogel, which is applied in medical science, prosthesis, etc., can solve the problems of low mechanical strength and fast degradation, and achieve the effects of simple method, good biocompatibility, and low cost of raw materials

Inactive Publication Date: 2009-08-12
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, collagen also has the characteristics of high-temperature gelation, and its disadvantages are too fast degradation in vivo and low mechanical strength, which limit the application of hydrogels.

Method used

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  • Method for preparing injectable chitosan/sodium glycerophosphate/collagen hydrogel
  • Method for preparing injectable chitosan/sodium glycerophosphate/collagen hydrogel
  • Method for preparing injectable chitosan/sodium glycerophosphate/collagen hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Dissolve 220mg chitosan in 10mL0.1mol.L -1 Mix well in the acetic acid solution to make a 2.2% (W / V) solution, and store in an ice bath; dissolve 2g of β-glycerophosphate sodium powder in 2mL triple-distilled water to make a 50% (W / W) solution; store in an ice bath , add 2mL sodium glycerophosphate solution dropwise into 10mL chitosan solution, stir well to obtain chitosan / sodium glycerophosphate solution; add 7.5mL tri-distilled water to 5mL collagen and mix evenly, then add the mixed solution to 312.5 0.1molL of mL -1 Collagen solution was prepared in NaOH solution; an equal volume of chitosan / sodium glycerophosphate solution and collagen solution were mixed under ice bath, and stirred for 10 minutes to obtain chitosan / sodium glycerophosphate / collagen hydrogel.

Embodiment 2

[0025] Dissolve 200mg chitosan in 10mL 0.1molL -1 Mix well in hydrochloric acid solution to make a 2% (W / V) solution, and store in an ice bath; dissolve 1g of β-glycerophosphate sodium powder in 1.5mL triple-distilled water to make a 40% (W / W) solution; Under the bath, add 1.5mL sodium glycerophosphate solution dropwise into 10mL chitosan solution, and stir thoroughly to obtain chitosan / sodium glycerophosphate solution; add 3mL triple-distilled water to 2mL collagen and mix evenly, and then mix the solution Add 125mL of 0.1molL -1 In the NaOH solution, the collagen solution is obtained; the prepared chitosan / sodium glycerophosphate solution and the collagen solution are mixed in an ice bath at a volume ratio of 10:1, and stirred for 10 minutes to obtain a gel that can be transformed into a gel at body temperature. chitosan / sodium glycerophosphate / collagen hydrogel.

Embodiment 3

[0027] Dissolve 150mg chitosan in 10mL 0.1molL -1 Mix well in the acetic acid solution to make a 1.5% (W / V) solution, and store in an ice bath; dissolve 1.64g of β-sodium glycerophosphate powder in 2mL triple-distilled water to make a 45% (W / W) solution; store in an ice bath Next, add 1mL sodium glycerophosphate solution dropwise into 6mL chitosan solution, stir well to obtain chitosan / sodium glycerophosphate solution; add 15mL triple distilled water to 10mL collagen and mix evenly, then add the mixed solution to 625mL 0.1molL -1 In the NaOH solution, the collagen solution is prepared; the prepared chitosan / sodium glycerophosphate solution and the collagen solution are mixed in an ice bath at a volume ratio of 1:10, stirred for 10 minutes, and obtained can be transformed into a gel at body temperature. chitosan / sodium glycerophosphate / collagen hydrogel.

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Abstract

The invention discloses a method for preparing injectable chitosan / sodium glycero-phosphate / collagen hydrogel, belonging to the field of biotechnology and tissue engineering; the invention particularly relates to a technology for preparing temperature sensitive type injectable material. The method is characterized in that 2.2% (W / V) of chitosan solution is mixed with 50% (W / V) of beta-sodium glycero-phosphate solution at low temperature to prepare chitosan / sodium glycero-phosphate solution; then, the solution is mixed with collagen solution following a volume ratio of 1:10-10:1 at low temperature; the mixture is retained in the liquid state at room temperature( 8 DEG C) and is turned into gel at body temperature within 12min. The invention has the beneficial effects that the chitosan / sodium glycero-phosphate / collagen hydrogel can be maintained in the liquid state at low temperature when pH value is within physiological range (pH value ranges from 7.0 to 7.2); the mixture is turned into gel when the temperature is raised to body temperature (37 DEG C). As ideal cell growth substrate collagen is added, the hydrogel features fine biocompatibility.

Description

technical field [0001] The invention belongs to the field of biotechnology and tissue engineering, and relates to a technology for preparing a temperature-sensitive injectable material, in particular to a preparation method of an injectable chitosan / sodium glycerophosphate / collagen hydrogel. Specifically, this method enables the preparation of a hydrogel that maintains a liquid state at low temperature and turns into a gel at body temperature, and the hydrogel has good biocompatibility. Background technique [0002] The basic idea of ​​tissue engineering is to inoculate functionally related cells on a three-dimensional biological scaffold material / extracellular matrix substitute with specific properties, and then transplant them into damaged tissues in vivo to repair damaged tissues. Three-dimensional scaffolds for tissue engineering are mainly divided into porous scaffolds ("hard" scaffolds) and injectable scaffolds ("soft" scaffolds). Injectable hydrogel scaffolds have th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/48A61L27/52A61L27/20A61L27/24
Inventor 刘天庆姜波关水张文宋克东葛丹李香琴马学虎陆瑞欣
Owner DALIAN UNIV OF TECH
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