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Preparation method of injectable hydrogel with photo-thermal property

An injectable and hydrogel technology, applied in the direction of medical preparations with non-active ingredients, medical preparations containing active ingredients, wave energy or particle radiation treatment materials, etc., can solve the problem of no photothermal/photodynamic effect Satisfaction and other issues

Active Publication Date: 2018-01-16
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it still has no photothermal / photodynamic effect, and cannot satisfy the effect of "one injection, multiple treatments"

Method used

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  • Preparation method of injectable hydrogel with photo-thermal property
  • Preparation method of injectable hydrogel with photo-thermal property

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1, preparation of dopamine graft modified sodium alginate (Alg-DA)

[0015] Sodium alginate (2g) was dissolved in 100mL PBS buffer solution (0.2mol / L, pH=5.5), after complete dissolution, EDC (3.88g) and NHS (4.68g) were added in sequence, stirred at room temperature for 45min, then Add 1.9 g of dopamine hydrochloride (DA·HCl) at a molar ratio of 1:1, pass nitrogen through a double-row tube, vacuumize, and repeat three times to exhaust the air in the flask to prevent DA oxidation. Stir and react at room temperature for 24 hours. After the reaction, precipitate with a large amount of ethanol to obtain a light brown solid, then wash and centrifuge three times with ethanol, dissolve the precipitate in deionized water, dialyze for three days, and freeze-dry to obtain dopamine-modified sodium alginate , labeled Alg-DA 1 .

[0016] It should be noted that the addition of dopamine hydrochloride (DA) in Example 1 is changed to 5.8g and 9.6g (i.e. n Alg / n DA 1:3...

Embodiment 2

[0017] Embodiment 2, preparation of polydopamine-wrapped gold nanorods (Au-PDA NRs)

[0018] Preparation of gold nanorods: HAuCl 4 ·3H 2 O (0.25mL, 0.01mol / L) was added into the CTAB (9.75mL, 0.1mol / L) solution, stirred slowly, and then the freshly prepared and frozen NaBH 4 The solution (0.6mL, 0.01mol / L) was quickly added to the mixed solution, stirred for 2min, and left at room temperature for at least 2h to obtain a seed solution. HAuCl 4 ·3H 2 O (0.5mL, 0.01mol / L), AgNO 3 (0.1mL, 0.01mol / L) and CTAB (10mL, 0.1mol / L) were mixed evenly, and ascorbic acid (0.08mL, 0.1mol / L) was added, and the color of the solution changed from yellow to colorless. Then add 0.2mL HCl (1.0mol / L) to adjust the pH of the system to 1-2, and finally add 0.024mL seed solution, stir slowly for 10s, place at room temperature for at least 6h, and centrifuge and wash three times with ultrapure water, and the obtained gold nanoparticles The rod (Au NRs) solution concentration was 0.2nmol / L.

[00...

Embodiment 3

[0022] Example 3, Preparation of chitosan / dopamine-modified sodium alginate temperature-sensitive hydrogel (CGP / Alg-DA@Au-PDA) doped with gold nanorods

[0023] Gold nanorods (Au-PDA) wrapped with polydopamine prepared above 0.25 NRs) after centrifugation and washing, directly dispersed into the Alg-DA solution, and ultrasonicated for 3 min, so that the composite gold nanorods could be uniformly dispersed in the Alg-DA solution, which was counted as Alg-DA@Au-PDA 0.25 . Then accurately pipette 3mL CS solution, add 0.5mL β-GP solution at 4°C, stir well, then add a certain amount of Alg-DA@Au-PDA 0.25 solution, stirred in an ice-water bath for 30 min. Place in a constant temperature water bath at 37°C, mix the solution to form a cross-linked composite gel, record the time of gel formation, and count the formed gel as CGP / Alg-DA@Au-PDA 0.25 Composite gel.

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Abstract

The invention discloses a preparation method of an injectable hydrogel with a photo-thermal property, and belongs to the field of functional materials. In a malignant tumor treatment, a patient shouldtake medicines for many times, while provided hydrogel only needs one injection. The preparation method comprises the following steps: at first, preparing a dopamine modified sodium alginate bio-macromolecule (Alg-DA); adjusting the gelation temperature of a temperature sensitive hydrogel (CGP) of chitosan (CS) and sodium beta-glycerophosphate (beta-GP) to the range of human physiological temperature so that the hydrogel is in a sol state at a room temperature and will become gel after arriving at a human body; then mixing Alg-DA and CGP to obtain an injectable temperature sensitive hydrogel,and doping a polydopamine wrapped gold nano rod (Au-PDA NRs), which is prepared in advance, into the hydrogel to obtain the injectable hydrogel with a photo-thermal property (CGP / Alg-DA@Au-PDA). Thehydrogel can fix Au NRs in tumors and carries out multiple photo-thermal conversions under laser radiation so as to treat tumors by utilizing high temperature. The injectable hydrogel has a potentialapplication value in the biomedicine field.

Description

technical field [0001] An injectable hydrogel with photothermal properties belongs to the technical field of functional materials. Background technique [0002] Used in the treatment of malignant tumors, the drug is transported to the lesion site through the circulatory system in the body, so as to achieve the purpose of treatment. However, there are a series of problems in the drug delivery system, such as non-specific distribution in the body, and the possibility of being quickly cleared from the body. As a local drug delivery system, hydrogel can be fixed at the lesion position to continuously and effectively release drugs. Therefore, the drug load and residence time can be selected according to the degree of disease progression, effectively solving the above problems. Due to the aggressiveness and drug resistance of malignant tumors, repeated administration of drugs is required during the treatment process, which not only limits the treatment efficiency but also brings ...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K9/06A61K47/34A61K47/36A61P35/00
Inventor 施冬健曾金凤杨雯迪李小杰陈明清
Owner JIANGNAN UNIV
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