Streptococcus mutans rnc gene mutant strain and application thereof

A technology for Streptococcus mutans and strains, which is applied in bacteria, microorganism-based methods, and medical preparations containing active ingredients, etc., can solve problems such as reports on the application of Streptococcus mutans rnc gene mutant strains, etc., and reduce the occurrence of rate, reduction of caries, and low cariogenic potential

Active Publication Date: 2017-01-18
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] So far, there have been no reports on the construction of rnc gene mutant strains of Streptococcus mutans and its application in the prevention and treatment of dental caries

Method used

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  • Streptococcus mutans rnc gene mutant strain and application thereof
  • Streptococcus mutans rnc gene mutant strain and application thereof
  • Streptococcus mutans rnc gene mutant strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: The acquisition and identification of "Streptococcus mutans HT120211CCTCC M2013211" of the present invention, see the procedure figure 1

[0037] 1. Construction and identification of "Streptococcus mutans HT120211CCTCC M2013211" of the present invention

[0038] 1.1 Cultivation of strains

[0039] Streptococcus mutans UA159 (ie: Streptococcus mutans UA159, referred to as "S.mutans UA159") obtained from the State Key Laboratory of Oral Disease Research in Chengdu, Sichuan Province, China was inoculated into bovine brain heart infusion (BHI) medium ( Sigma-Aldrich Corp, Saint Louis, MO, USA), the bacteria were routinely subcultured overnight and cultured in an DY-2 anaerobic incubator (37°C, 5% CO2, 10% H2, 85% N2; Zhejiang, China) for later use.

[0040] 1.2 Construction of Streptococcus mutans HT120211 mutant strain

[0041] Check the NCBI database to obtain the DNA sequence of the rnc gene and its upstream and downstream fragments, and design primers (rnc-P1, rnc-P2...

Embodiment 2

[0074] Example 2: Comparison of the cariogenic ability of "Streptococcus mutans HT120211CCTCC M2013211" of the present invention in a rat model of human caries and the male parent strain Streptococcus mutans UA159

[0075] 1. In the rat model of human dental caries, Streptococcus mutans UA159 and Streptococcus mutansHT120211CCTCC M2013211 were inoculated into 10ml BHI (supplemented with 1% sucrose) overnight, centrifuged, and the supernatant was discarded and resuspended in 10ml fresh BHI medium. Aspirate 100 μl of the bacterial suspension in 2ml BHI medium and continue to culture to an optical density value of 0.4 (OD600).

[0076] In the in vivo experiment, the animals were kept for 3 weeks after the successful colonization of the strains, CO 2 The rats were killed by asphyxiation method, the mandible specimens were taken, and sterilized normal saline was gently rinsed 3 times. The right mandible was placed in a sterile 15ml test tube containing 5ml RNA tissue sample protection s...

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Abstract

The invention discloses a (i) rnc (/i) gene mutant strain of (i) Streptococcusmutans (/i) strain in long chain shape and with low cariogenic ability and application thereof to the prevention of dental caries. The mutant strain is obtained through construction, screening and identification of Streptococcusmutans UA159, and has a preservation number of CCTCCM2013211, classification nomenclature of (i) Streptococcusmutans (/i) HT120211. The (i) rnc (/i) gene mutant strain of (i) Streptococcusmutans (/i) strain provided by the invention can be prepared into a micro ecological preparation as an alternative strain of Streptococcusmutans, and can adjust the oral dysbacteriosis, reduce the number of Streptococcusmutans with high cariogenic ability, and prevent dental caries.

Description

Technical field [0001] The present invention relates to a long-chain-shaped, low-cariogenic rnc gene mutant strain of Streptococcus mutans obtained through construction, screening and identification of Streptococcus mutans UA159 and its application in preventing dental caries. The rnc gene mutant strain of Streptococcus mutans can be made into probiotics, which can be used as an alternative strain of Streptococcus mutans to adjust the oral flora imbalance, reduce the number of Streptococcus mutans with high cariogenic ability, and prevent the occurrence of caries. It belongs to the prevention of caries. Technical field. Background technique [0002] The incidence of dental caries is high and it is one of the most common human infectious diseases. The WHO survey found that about 5 billion people worldwide suffer from dental caries. In my country, the third national oral epidemiological survey in 2005 showed that the prevalence of dental caries among 5-year-old children was about ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/744A61P1/02C12R1/46
Inventor 胡涛杨英明李克增张茹刘思茗毛梦莹雷蕾阳燕
Owner SICHUAN UNIV
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