Tissue culture method for centranthera grandiflora

A technology of flax herb and tissue culture, which is applied in the field of plant tissue culture, can solve the problems of low reproduction coefficient and serious explant pollution, and achieve the effects of high reproduction coefficient, high seed survival rate and strong operability

Active Publication Date: 2017-08-18
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Predecessors have carried out relevant research on the key technical links of tissue culture of Flaxa grandiflora, but the problems such as serious explant pollution and low reproduction coefficient in the tissue culture process have not been effectively solved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for tissue culture of flax herbaceous flax, which first adopts in vitro tissue culture to obtain tissue culture seedlings, then hardens the seedlings and transplants to obtain flax flax grandiflorum seedlings, wherein the in vitro tissue culture comprises the following steps:

[0031] (1), explant selection and pretreatment steps: select the uncracked flax seeds of seed pods as starting material, wipe the surface with 75% alcohol, take out the seed shells and use them as explants for tissue culture and propagation body. Place the explants in a sterilized 1.5ml centrifuge tube, add 1ml of sterile water to soak for 1-2h, after the seeds absorb enough water, centrifuge at 1000rpm for 3-5min, pour off the supernatant, and soak in 75% alcohol Carry out 30-60s disinfection treatment in the solution, then carry out 8-10min sterilization treatment in the mercuric chloride solution that weight volume ratio concentration is 0.1%, then rinse 6-8 times with sterile water, ...

Embodiment 2

[0036] A method for tissue culture of flax herbaceous flax, which first adopts in vitro tissue culture to obtain tissue culture seedlings, then hardens the seedlings and transplants to obtain flax flax grandiflorum seedlings, wherein the in vitro tissue culture comprises the following steps:

[0037] (1), explant selection and pretreatment steps: select the uncracked flax seeds of seed pods as starting material, wipe the surface with 75% alcohol, take out the seed shells and use them as explants for tissue culture and propagation body. Place the explants in a sterilized 1.5ml centrifuge tube, add 1ml of sterile water to soak for 1-2h, after the seeds absorb enough water, centrifuge at 1000rpm for 3-5min, pour off the supernatant, and soak in 75% alcohol Carry out 30-60s disinfection treatment in the solution, then carry out 8-10min sterilization treatment in the mercuric chloride solution that weight volume ratio concentration is 0.1%, then rinse 6-8 times with sterile water, ...

Embodiment 3

[0042] A method for tissue culture of flax herbaceous flax, which first adopts in vitro tissue culture to obtain tissue culture seedlings, then hardens the seedlings and transplants to obtain flax flax grandiflorum seedlings, wherein the in vitro tissue culture comprises the following steps:

[0043] (1), explant selection and pretreatment steps: select the uncracked flax seeds of seed pods as starting material, wipe the surface with 75% alcohol, take out the seed shells and use them as explants for tissue culture and propagation body. Place the explants in a sterilized 1.5ml centrifuge tube, add 1ml of sterile water to soak for 1-2h, after the seeds absorb enough water, centrifuge at 1000rpm for 3-5min, pour off the supernatant, and soak in 75% alcohol Carry out 30-60s disinfection treatment in the solution, then carry out 8-10min sterilization treatment in the mercuric chloride solution that weight volume ratio concentration is 0.1%, then rinse 6-8 times with sterile water, ...

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Abstract

The invention discloses a tissue culture method for centranthera grandiflora. The method includes the steps of: (1) explant selection and pretreatment; (2) seed germination culture; (3) differentiation and proliferation culture of seedlings; (4) induced rooting culture of segmented seedlings; and (5) seedling exercising and transplantation. The method provided by the invention improves the seed disinfection technique, multiplication culture technique and strong seedling rooting technique in a centranthera grandiflora tissue culture process, and solves the problems of easy seed pollution, low propagation coefficient, and easy browning, etc. The centranthera grandiflora tissue culture seedlings cultured by the method have the advantages of high propagation coefficient, fast growth rate and high transplantation survival rate. In addition, the method provided by the invention is simple and practicable, and has strong operability and reproducibility.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a culture method, in particular to a tissue culture method of flax herb. Background technique [0002] Centranthera grandiflora Bench., a plant of the genus Centranthera grandiflora Bench. It is also known as Huaxuedan, Red Root Wild Broad Bean, Wild Broad Bean Root, Little Red Medicine, Ganoderma Lucidum, etc. It is mainly distributed in Yunnan, Guizhou and Guangxi in my country And other places and India, Myanmar and other places. The biological characteristics of flaxgrass are that the whole plant is covered with bristles, the fibrous roots are rusty red, the stem is rigid and branched; the leaves are opposite, without petioles, oblong or strip-shaped, and the entire margin; the flowers are axillary, and the calyx is Buddha Flame-shaped, bracts are leaf-shaped, corolla is tubular, brownish yellow. It is a famous and precious local traditional Chinese medicine in Yu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 陈敏敏叶正文张学英张建军张永春殷丽青杨柳燕
Owner SHANGHAI ACAD OF AGRI SCI
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