SNP molecular marker associated with pig growth traits

A growth trait and molecular marker technology, applied in the determination/inspection of microorganisms, recombinant DNA technology, biochemical equipment and methods, etc., can solve the problems of low sample content, difficulty in obtaining candidate genes, and easy questioning of research results, etc. achieve high sensitivity and specificity

Active Publication Date: 2019-02-22
湖北金旭农业发展股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, there have been more and more genome-wide association analyzes of pig growth traits, but most of the st

Method used

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  • SNP molecular marker associated with pig growth traits
  • SNP molecular marker associated with pig growth traits
  • SNP molecular marker associated with pig growth traits

Examples

Experimental program
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Embodiment 1

[0030] Example 1: Genotyping Detection

[0031] (1) Using the kit method (spin column type) to extract the genomic DNA of the ear tissue or tail tissue of pig growth traits-related populations

[0032] 1) Cut the ear tissues or tail tissues of pigs related to growth traits (such as Large White pigs, Landrace pigs, Duroc pigs and Pietrain pigs) to a paste with ophthalmic scissors, and add 200ulGA (the kit is commercially available, the article number is TIANGEN, DP304-03), and shake and mix; then put it into a 56°C water bath for overnight digestion;

[0033] 2) Add 200ul buffer solution GB to the digested tissue sample (the kit is commercially available, the article number is TIANGEN, DP304-03, the GB reagent comes with the kit, the same below), fully invert and mix, and place at 70°C After 10 minutes, the solution should be clear and centrifuged briefly to remove water droplets on the tube wall;

[0034] 3) Add 200ul of absolute ethanol, shake and mix well for 15 seconds, a...

Embodiment 2

[0042] Embodiment 2: Affx-114707063 molecular marker typing method and its application in pig growth traits correlation analysis (1) Affx-114707063 molecular marker typing result and growth traits (for example: correcting 100KG day weight gain traits and correcting 100KG day age traits) association analysis

[0043] The experimental pigs used for the association analysis of genotypes and growth traits were from two independent purebred pig groups, including Large White pigs, Landrace pigs, Duroc pigs and Pietrain pigs, all of which were conventional breeds). The DNA used for genotyping consists of purebred Large White pigs, Landrace pigs, Duroc pigs, and Pietrain pigs (the purebred Large White pigs, Landrace pigs, Duroc pigs, and Pietrain pigs referred to in the text of the instructions and tables are referred to as " Pig") ear or tail sample extraction. Using the method based on the multi-marker association model, using the individual's gender and session as fixed effects, t...

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Abstract

The invention belongs to the technical field of preparation of pig molecular markers, and in particular relates to an SNP (single nucleotide polymorphism) molecular marker associated with pig growth traits. The pig growth traits comprise corrected 100KG daily gain traits and corrected 100KG daily age traits. The molecular marker is cloned from a gene fragment with the accession number of Affx-114707063, and the gene is genotyped by a gene chip technology to obtain the molecular marker associated with the pig growth traits, and the sequence of the molecular marker is shown in SEQ ID NO:1. An A/T allelic mutation exists at the 51st base of the sequence, thereby resulting in a polymorphism in the nucleotide sequence shown in SEQ ID NO:1; and when the 51st nucleotide of the fragment is A, a pig is indicated to have a faster growth rate. The invention further discloses a method for screening the molecular marker associated with the pig growth traits and application of the method in associated analysis. A novel marker resource is provided for the auxiliary selection of the pig growth traits.

Description

technical field [0001] The invention relates to the technical field of preparation of pig molecular markers, in particular to SNP molecular markers associated with pig growth traits. The growth traits of pigs include the traits of correcting 100KG daily weight gain and correcting 100KG daily age traits. The molecular marker of the invention can be used for auxiliary selection and prediction of pig growth traits. Background technique [0002] Domestic pigs are one of the earliest domesticated animals domesticated by humans. Domestic pigs are the main species of meat consumed by humans, and their pork production ranks first among all domestic animals (Larson G, Liu R, Zhao X, et al. Patterns of East Asian pig domestication , migration, and turnover revealed by modern and ancient DNA [J]. Proceedings of the National Academy of Sciences of the United States of America, 2010, 107(17): 7686-7691.). my country has a large population, and the demand for pork is huge. It is an urge...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 赵书红唐振双李新云尹立林南九红许婧雅梅全顺彭潇余梅刘小磊项涛
Owner 湖北金旭农业发展股份有限公司
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