Trace amount environment incretion jam object molecular imprinting film substrate and preparation method and application thereof

Abstract

The invention provides an endocrine interferent molecularly imprinted membrane substrate in a trace environment with rapid detection speed and high sensitivity, the preparation method and the application thereof. The molecularly imprinted membrane substrate comprises a gold quartz crystal substrate, and multi-layer nano-materials that alternate with each other and environmental endocrine interferent molecularly imprinted polymer are formed on the surface of the gold quartz crystal substrate. The preparation method comprises the following steps: selecting functional monomer that can be synthesized with the environmental endocrine interferent into the molecularly imprinted polymer; preparing the molecularly imprinted polymer solution; and modifying the nano-material and the molecularly imprinted polymer on the surface of the gold quartz crystal substrate. The invention applies the surface modification technology to the preparation of the molecularly imprinted membrane substrate, so as to allow the controllability over the preparation of the nano-scale synergetic EDCs molecularly imprinted membrane substrate. A method for detecting the environmental endocrine is rapid and simple, and the reaction and the result are automatically recorded and completed by an instrument with good repeatability, thereby facilitating the on-site detection.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a trace environmental endocrine disruptor molecularly imprinted membrane substrate and a preparation method and application thereof. Background technique [0002] Endocrine Disrupting Chemicals (EDCs for short) refer to a class of foreign substances that interfere with the normal behavior of organisms and the synthesis, storage, secretion, in vivo transportation, combination, and clearance of normal hormones related to reproduction and development. Environmental endocrine disruptors are divided into the following categories: polychlorinated biphenyls, dioxins, alkylphenols, dibutyl phthalates, metals, organochlorine compounds, environmental hormones, phytoestrogens, Fungal estrogens. [0003] At present, EDCs have become a new generation of environmental pollutants that urgently need to be treated. A small amount of EDCs can affect normal hormones, interfere with en...

AI Technical Summary

Problems solved by technology

[0005] 1. Uterine weight gain test and uterine calcium-binding protein CaBP-9k mRNA expression detection and protein analysis are often used in in vivo tests. This method is sensitive and specific, but its cost is high and its sensitivity is low, so it is not suitable for large-scale rapid screening. ;

[0006] 2. In vitro tests mainly include E-Screen method, receptor competition test, etc. The specificity of the test itself is poor, and false positive results are prone to occur;

[0007] 3. The EDCs instrument detection method established with the help of large precision instruments such as HPLC or LC-MS combined technology has high sensitivity for the detection of EDCs, but this method cannot identify the structure of the analyte at one time, often Need to use GC-MS to confirm the structure, the technical cost is high, the traditional method generally takes a long time, some even up to several hours, the operation is complicated, and cannot be used for rapid on-site detection

[0008] 4. For the detection and analysis of EDCs, the above methods generally have shortcomings such as low detection sensitivity, high cost, false positive results, complicated detection process, and not suitable for rapid on-site detection, so they cannot meet the needs of actual detection.

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