Micro-fluidic chip for multi-cell co-culture and preparation method thereof

Abstract

The invention discloses a micro-fluidic chip for multi-cell co-culture and a preparation method of the micro-fluidic chip. The micro-fluidic chip comprises an on-membrane cell culture layer, a porous membrane and an under-membrane cell culture layer, the cell culture layer can be provided with a plurality of cell culture channels, and each cell culture channel comprises a cell culture cavity, and a perfusion channel, an outflow channel and a cell injection port which are respectively connected with the cell culture cavity. The micro-fluidic chip disclosed by the invention realizes multi-cell co-culture, and can be used for researching the influence of drugs on different organs and tissues and the mutual action of the drugs.

Description

technical field [0001] The invention belongs to the fields of cell engineering and biomimetic organ engineering, and particularly relates to a microfluidic chip applied to the simultaneous cultivation of multiple cells. Background technique [0002] In order to better reveal the relationship between drugs and diseases, people have used different models to discover the pathogenesis of diseases and the effects of drugs. At present, the most commonly used systems for simulating the human body are mammalian models and cell models. Animal models are in vivo models. In practical applications, there are long experimental periods, large labor, high costs and ethical issues, and high-throughput analysis cannot be performed. thereby limiting its use. In addition, due to numerous interfering factors in in vivo experiments, it is difficult to accurately analyze the influence of a certain factor on the occurrence of the disease, which increases the difficulty of predicting drug response...

AI Technical Summary

Problems solved by technology

Therefore, the complex and diverse structural design of these chips has not been effectively used to simulate the interaction between systems and multiple organs.

Chinese patent CN112760225A discloses a culture system for evaluating the early implantation ability of bovine embryonic cells by using transwell chambers and cell culture plates stacked up and down. Materials that simulate endometrial cell matrix are arranged on the porous structure at the bottom of the chamber to Close the hole, culture layered endometrial cells in the culture hole below the small chamber, and communicate with the embryonic cells in the small chamber. Although the patent provides a bionic environment for embryo implantation (including endometrial cells that can be degraded matrix), but the Transwell culture system is generally static and cannot simulate the impact of the dynamic environment of the fluid in the body on the function of the body

[0005] Facing the problem that the nephrotoxicity risk of drugs is often underestimated in preclinical experiments due to the neglect of the role of drug absorption in the assessment of nephrotoxicity, it is urgent to develop a method that can not only reflect drug absorption, but also be more accurate. An in vitro model to predict its nephrotoxicity, thereby better promoting drug development

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