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Personalized tumor assembly construction method and device based on droplet microfluidic technology

A technology for constructing methods and assemblies, which can be applied in the directions of supporting/immobilizing microorganisms, sterilization methods, chemical instruments and methods, etc., and can solve problems affecting drug screening stability and consistency, size, uncontrollable fusion morphology, Problems such as poor formability and uniformity, to achieve the effect of high-throughput screening, controllable microsphere size, and prevention of deposition

Active Publication Date: 2021-11-02
TSINGHUA UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, there are still key problems in tumor organoid models: organoid components are single, and blood vessels, CAFs, and immune microenvironment are all lost with culture
The size and fusion shape of the quasi-assemblies formed by the co-culture method are uncontrollable, and it is also time-consuming and labor-intensive.
Although the extrusion 3D bioprinting method can achieve rapid assembly of quasi-assemblies, the formability and uniformity are poor, which will affect the stability and consistency of drug screening.

Method used

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  • Personalized tumor assembly construction method and device based on droplet microfluidic technology
  • Personalized tumor assembly construction method and device based on droplet microfluidic technology
  • Personalized tumor assembly construction method and device based on droplet microfluidic technology

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Embodiment Construction

[0052] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0053] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0054] Such as figure 1 As shown, the present invention provides a method for constructing tumor-like assemblies based on droplet microfluidic technology, including: preparation of individualized tumor microtissues, forming microspheres of the like-assembly, solidification of the microspheres, and collection of the like-assembly.

[0055] The preparation method of personalized tumor microtissue ink 103 is as follows: collect fresh tumor tissue after clinical operation, the tissue is not smaller than 1.5×1.5×1.5cm, wash the tumor tissue with PBS containing penicillin and streptomycin for 3 times, and then use The ophthalmic scissors were mechanically chopped, and the tumor microtissue was digested with 2mg / mL collagenase type I ...

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Abstract

The invention discloses a personalized tumor assembly construction method and a personalized tumor assembly construction device based on a droplet microfluidic technology. The construction method comprises the following steps: S1, dispersing personalized tumor micro-tissues from a clinical source into bio-ink to serve as a dispersion phase; S2, respectively injecting the dispersed phase and the continuous phase into the T-shaped channel of the micro-fluidic chip to obtain the tumor assembly microspheres at the outlet of the T-shaped channel, wherein the dispersed phase inlet is perpendicular to the continuous phase inlet and the continuous phase outlet; S3, carrying out curing molding on the tumor assembly microspheres to obtain the personalized tumor assembly. According to the method disclosed by the invention, the assembly-like microspheres with good uniformity can be continuously produced, the microspheres are controllable in size, stable in form, good in biocompatibility and good in cell survival and proliferation, personalized tumor-like tissues with tumor microenvironments (microvessels, fibroblasts, immune cells and the like) can be formed after culture, and high-throughput screening of drugs can be realized.

Description

technical field [0001] The invention relates to a method and device for constructing a personalized tumor assembly based on droplet microfluidic technology, and belongs to the technical field of biomanufacturing. Background technique [0002] Cancer is the main cause of death in the world. In 2020, there will be 19.29 million new cancer cases worldwide and 9.96 million cancer deaths worldwide. Cancer research and treatment is still a major problem in scientific research. One of the key reasons why cancer has not been successfully cured so far is the heterogeneity of tumors, and the tumor microenvironment is a key factor affecting tumor heterogeneity, which will significantly affect tumor development, metastasis and drug resistance. The tumor microenvironment is the environment on which tumor cells grow and develop, mainly including the immune microenvironment and non-immune microenvironment, while the non-immune microenvironment mainly includes tumor-associated fibroblasts (...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12M3/06C12M1/38C12M1/36C12M1/34C12M1/12B01L3/00
CPCC12N5/0693C12M23/16C12M29/04C12M41/00C12M41/22B01L3/5027C12N2513/00C12N2533/80B01L2300/1861B01L2200/0647Y02P10/25
Inventor 熊卓张艳梅张婷骆浩
Owner TSINGHUA UNIV
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