Preparation method of high-throughput liquid crystal detection platform for screening enzyme inhibitor by inducing aptamer to release through enzyme catalysis

A detection platform and aptamer technology, which is applied in the field of analysis and detection, can solve problems such as restricting development, and achieve good effects, high sensitivity, and good selectivity

Active Publication Date: 2021-06-11
SHANDONG ANALYSIS & TEST CENT
View PDF7 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The inventors found that there is currently no simple platform that can quickly and ef

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of high-throughput liquid crystal detection platform for screening enzyme inhibitor by inducing aptamer to release through enzyme catalysis
  • Preparation method of high-throughput liquid crystal detection platform for screening enzyme inhibitor by inducing aptamer to release through enzyme catalysis
  • Preparation method of high-throughput liquid crystal detection platform for screening enzyme inhibitor by inducing aptamer to release through enzyme catalysis

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0053] In a second aspect of the invention, a method of preparing a high-throughput liquid crystal biosensor of the enzyme inhibitor is provided by an enzyme catalytic induction adapter, including:

[0054] Precessibility of glass substrate;

[0055] Liquid crystal molecules were added to a surfactant (otab) chloroform solution, and then the chloroform was removed with nitrogen to obtain a mixture of liquid crystal and OTAB, and added to the surface of the copper network;

[0056]The drug to be tested is transformed with the substrate to the substrate, the small molecule substrate, the adapter DNA is mixed, and it is added to the copper network, which is obtained.

[0057] The present invention uses copper web method to observe the optical phenomenon of liquid crystal, high sensitivity, short time consumption, and simple preparation method.

[0058] In a third aspect of the invention, a method of screening an enzyme inhibitor using the above-described liquid crystal biosensor high...

Example Embodiment

[0109] Example 1:

[0110] Using enzyme-catalytic induction of adapter release screen to detect non-Bishan enzyme inhibitory efficiency

[0111] The liquid crystal sensor used is prepared as follows:

[0112] A, soaked with a glass piece at 75 ° C for 30 min, then washed with a large amount of ultrapure water, methanol, ethanol, and dried in an oven at 120 ° C after drying with nitrogen.

[0113] B. At room temperature, the glass bubble is 20 minutes in DMOAP in DMOAP in a volume fraction of 1%, and then washed with ultrapure water, and the glass substrate can be obtained by blowing with nitrogen.

[0114] Move several copper nets to the resulting glass substrate.

[0115] Otab was dissolved in chloroform and configured to concentrate a chloroform solution of 1 mmotab.

[0116]C, mixed 10 μl of 5 CB molecules with 90 μl of 1 mm OTAB solution, vortex 30s, and the mixture was finally concentrated in a mixture of 100 μm, and then heated in an oven at 75 ° C for 4 h. The resulting mix...

Example Embodiment

[0124] Example 2: Synthesis of a variety of xanthine enzyme inhibitors and use enzyme-catalytic induction adapter release screening of a high-throughput liquid crystal detection platform for xanthantine inhibitors Screening effective drugs

[0125] Preparation of Liquid Crystal Sensor is the same as in Example 1

[0126] S1, the compound to be detected is pre-mixed with the xanthine oxidase and incubated for 30 min; then incubated with xanthine for 30 min, and then incubated with xanthine adapter DNA for 60 min.

[0127] S2, the resulting mixture was removed from the liquid crystal sensor to observe using a polarizing microscope.

[0128] The synthetic xanthine enzyme inhibitors are benzene triazole compounds and their derivatives. Three suitable enzyme inhibitors can be selected by the above liquid crystal test platform, and three applicable enzyme inhibitors can be effectively screened.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of analysis and detection, and relates to a preparation method of a high-throughput liquid crystal detection platform for screening an enzyme inhibitor by inducing aptamer release through enzyme catalysis. The high-throughput liquid crystal detection platform comprises the following components of: a glass slide, a copper net, liquid crystal molecules, a surfactant, aptamer coding DNA, a substrate and a substrate specific invertase. The components are utilized to modify a liquid crystal biosensor, efficient screening of the enzyme inhibitor is achieved, and the effect of the platform is better than that of an existing screening method at present. The detection method provided by the invention has the advantages of high sensitivity and short time consumption; and the preparation method has the advantages of simplicity, good selectivity, rapidness, simplicity, low cost and no need of large-scale instruments, and has a good application prospect in high-throughput screening of enzyme inhibitors.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a liquid crystal biosensor for screening enzyme inhibitors and high-throughput screening for enzyme inhibitors. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily to be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] An enzyme inhibitor is a substance that can inhibit the activity of a specific enzyme related to a certain disease in an organism, thereby obtaining a curative effect. Enzyme inhibitors are substances that specifically act on certain groups of enzymes to reduce the activity of the enzyme or even completely inactivate the enzyme to reduce the rate of the enzymatic reaction. At present...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/84
CPCG01N21/84
Inventor 胡琼政武文丽夏爽
Owner SHANDONG ANALYSIS & TEST CENT
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap