Micro photometer

Abstract

A micro photometer, comprising a micro photometric cell (1), a front support (2), a back support (3), a front reel (5), a back reel (6) and a sampling mechanism (7); a thin plastic film strip (4) wound on the front reel (5) sequentially moves through the front support (2), the sampling mechanism (7), the micro photometric cell (1) and the back support (3) and is connected to the back reel (6). The micro photometer achieves high-throughput sample screening, can run automatically without maintenance, and only needs replenishing the thin film strip regularly.

Description

technical field [0001] The invention relates to the technical field of measurement, in particular to a microphotometer. Background technique [0002] A UV-Vis spectrophotometer is an instrument that transmits light through a sample and analyzes its absorption spectrum to qualitatively or quantitatively analyze components. Generally, it is composed of light source, monochromator, sample cell and detector. [0003] Traditional UV-visible spectrophotometer products use a standard 10mm optical path liquid sample cell, and the amount of liquid to be tested is generally more than 2mL. When used in life sciences, especially when measuring protein, DNA and RNA content, the sample is generally precious and the concentration is relatively high, so it must be diluted before using the 10mm sample cell. Dilution consumes solvent, the operation is cumbersome, and the sample cannot be recycled after dilution. In recent years, spectrophotometers for ultra-micro sample cells have appeared...

AI Technical Summary

Problems solved by technology

[0006] In the above methods, a mechanical opening and closing mechanism is required. The distance between the planes after closing is the measurement optical path. Generally, the mechanical positioning pin or positioning surface is used to ensure the distance. From opening to closing, the mechanical positioning pin or positioning If the surface is worn, mixed with dust or liquid, it will affect the positioning accuracy, and then affect the optical path, causing measurement errors

[0007] On the other hand, the liquid drops on the glass or quartz surface. After the measurement is completed, the liquid droplets need to be wiped off manually, which will bring a series of problems: the manual wiping action is slow, and high-throughput and automatic measurement cannot be achieved; manual operation has errors , easy to wipe clean, especially protein samples have strong adhesion, easy to remain on the glass surface, affect the absorbance measurement, and pollute the next drop of the sample; wipe the surface for a long time, the surface wear and tear, not only optical The performance deteriorates, the hydrophobicity and anti-adhesion will decrease, and regular maintenance is required, otherwise the performance of the instrument will be affected

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