Novel method for detecting lead ions based on sensitivity of DNA-MOF material

A sensitive detection, lead ion technology, applied in the field of lead ion detection, can solve the problems of structural loss of catalytic activity, thermal, acid and alkali instability, low natural enzyme content, etc. Good capacitive effect

Inactive Publication Date: 2017-01-11
UNIV OF JINAN
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since most enzymes are proteins, they are unstable to heat, acid, and alkali, and their structure changes to make them lose their catalytic activity
In addition, the content of natural enzymes in organisms is very low, and it is difficult to obtain natural enzymes in large quantities through purification, so the price is expensive, which greatly limits its practical application

Method used

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  • Novel method for detecting lead ions based on sensitivity of DNA-MOF material

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Experimental program
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preparation example Construction

[0035] (2) Preparation of MOF materials: Take 30.22 mg 5,10,15,20-tetrakis(4-methoxyphenyl)porphyrin, 35.45 mg FeCl 3 ·6H 2 O and 0.8 mL 0.1 mol L -1 The HCl ethanol solution was added to a mixed solution of 3 mL N,N'-dimethylformamide and 6 mL ethanol for ultrasonic treatment for 10 min, then transferred to a 40 mL autoclave and reacted at 150 °C for 36 h to obtain Fe- Porphyrin MOF materials;

[0036] (3) Preparation of Au-MOF material: Dissolve 4.0 mg of the iron-porphyrin MOF material prepared in step (2) into 1 mL of ethanol, and add 0.20 mL of 1.00 mol L -1 A mixture of ammonia solution and 11.3 mL ethanol, then add 1.5 mL6.0 mmol·L -1 Tetraethyl orthosilicate solution, stirred and reacted at room temperature for 2 h, the resulting mixture was centrifuged and washed three times with ethanol, and then the obtained solid was added to 2 mL of nano-gold solution, stirred and reacted for 12 h to obtain the Au-MOF material ;

[0037] (4) Preparation of GR-Au-MOF material:...

Embodiment 1

[0040] Example 1 (industrial wastewater)

[0041] (1) Print out the working area of ​​the required three-electrode system by wax printing, print the Ag / AgCl reference electrode and carbon contrast electrode on the working area by screen printing, and then modify the gold seeds on the working electrode, drop Add a mixed solution of chloroauric acid and sodium borohydride to obtain gold-paper chip working electrode Au-PWE;

[0042] (2) Preparation of MOF materials: Take 30.22 mg 5,10,15,20-tetrakis(4-methoxyphenyl)porphyrin, 35.45 mg FeCl 3 ·6H 2 O and 0.8 mL 0.1 mol L -1 The HCl ethanol solution was added to a mixed solution of 3 mL N,N'-dimethylformamide and 6 mL ethanol for ultrasonic treatment for 10 min, then transferred to a 40 mL autoclave and reacted at 150 °C for 36 h to obtain Fe- Porphyrin MOF materials;

[0043] (3) Preparation of Au-MOF material: Dissolve 4.0 mg of the iron-porphyrin MOF material prepared in step (2) into 1 mL of ethanol, and add 0.20 mL of 1.00...

Embodiment 2

[0048] Example 2 (apple juice)

[0049] (1) Print out the working area of ​​the required three-electrode system by wax printing, print the Ag / AgCl reference electrode and carbon contrast electrode on the working area by screen printing, and then modify the gold seeds on the working electrode, drop Add a mixed solution of chloroauric acid and sodium borohydride to obtain gold-paper chip working electrode Au-PWE;

[0050] (2) Preparation of MOF materials: Take 30.22 mg 5,10,15,20-tetrakis(4-methoxyphenyl)porphyrin, 35.45 mg FeCl 3 ·6H 2 O and 0.8 mL 0.1 mol L -1 The HCl ethanol solution was added to a mixed solution of 3 mL N,N'-dimethylformamide and 6 mL ethanol for ultrasonic treatment for 10 min, then transferred to a 40 mL autoclave and reacted at 150 °C for 36 h to obtain Fe- Porphyrin MOF materials;

[0051] (3) Preparation of Au-MOF material: Dissolve 4.0 mg of the iron-porphyrin MOF material prepared in step (2) into 1 mL of ethanol, and add 0.20 mL of 1.00 mol L -1...

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Abstract

The invention discloses a novel method for detecting lead ions based on the sensitivity of a DNA-MOF material. The method comprises the following steps: preparing a gold-paper chip working electrode Au-PWE; synthesizing Au-MOF from 5,10,15,20-tetra(4-methoxy phenyl)porphyrin, ferric chloride and nanogold; carrying out functionalization by virtue of GR DNA, so as to synthesize a GR-Au-MOF material; modifying the prepared Au-PWE with HP DNA, and dropwise adding a GR-Au-MOF solution mixed with 3,3',5,5'-tetramethyl benzidine, hydrogen peroxide and a sample solution to HP modified Au-PWE; and connecting the modified chip to an electrochemical working station by virtue of a three-electrode system, and detecting the concentration of lead ions by virtue of a differential pulse voltammetry. The novel method for detecting the lead ions is high in sensitivity and good in selectivity, and the real-time monitoring of samples can be realized.

Description

technical field [0001] The invention relates to the technical field of lead ion detection, more specifically a new method for sensitive detection of lead ions based on DNA-MOF materials, and the invention also relates to the sensitive detection of lead ions by differential pulse voltammetry. Background technique [0002] Lead ion content is an important indicator in environmental monitoring, because lead ions enter the human body through the food chain, and when the content reaches a certain concentration, it will cause a certain degree of damage to the human body, such as dementia in children, slow brain development, immune system damage, and severe cases. It can cause death, so it is necessary to seek a highly efficient and sensitive detection method. With the continuous improvement of research, various sensors for detecting lead ions have been established by using highly efficient DNase, but this DNase has the disadvantages of poor stability, acid resistance and alkali re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/327
CPCG01N27/48G01N27/3278
Inventor 王秀颜梅马超杨春蕾张彦于京华葛慎光
Owner UNIV OF JINAN
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