Electrochemical sensor for mercury ion detection, and manufacturing method and detection method thereof

A manufacturing method and sensor technology are applied in the field of biological analysis to achieve the effects of simple manufacturing method, high specificity and simple steps

Active Publication Date: 2013-05-15
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the development of Hg with extremely high sensitivity and selectivity and a sufficiently wide kinetic range 2+ The detection method is still a challenge to the majority of researchers

Method used

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  • Electrochemical sensor for mercury ion detection, and manufacturing method and detection method thereof
  • Electrochemical sensor for mercury ion detection, and manufacturing method and detection method thereof
  • Electrochemical sensor for mercury ion detection, and manufacturing method and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Construction of Hg when the whole thymine (T) oligonucleotide chain assembled on the surface of the gold electrode is low density 2+ Detection of electrochemical sensors.

[0038] Soak an unmodified clean gold electrode in 10 mM Tris, 0.2 M NaCl, pH=8.0 containing 1 μM probe 1 that has been reduced with tris[2-carboxyethyl]phosphine (TCEP) for 1 h, and place it at room temperature for 1 h . The assembled electrode was rinsed twice with 10mM Tris, 0.5M NaCl, pH = 8.0, then placed in an aqueous solution containing 1mM mercaptohexanol (MCH) to block for 30 minutes, and then washed with 10mM Tris, 0.5M NaCl, pH = 8.0 Wash three times, then react with 500nM probe 2 in 1 / 15M PB, 0.3M NaCl, pH=7.4 solution for 2h, 25°C. Then wash the electrode with 1 / 15M PB, 0.1M NaCl, pH=7.4 solution for three times and store it at 4°C for future use.

Embodiment 2

[0039] Example 2: Construction of Hg when the whole thymine (T) oligonucleotide chain assembled on the surface of the gold electrode is medium density 2+ Detection of electrochemical sensors.

[0040] Soak an unmodified clean gold electrode in 10 mM Tris containing 1 μM probe 1 that has been reduced with tris[2-carboxyethyl]phosphine (TCEP) for 1 h, 1.0 M NaCl, pH=8.0, and place at room temperature, Self-assemble overnight. The assembled electrode was rinsed twice with 10mM Tris, 0.5M NaCl, pH = 8.0, then placed in an aqueous solution containing 1mM mercaptohexanol (MCH) to block for 30 minutes, and then washed with 10mM Tris, 0.5M NaCl, pH = 8.0 Wash three times, then react with 500nM probe 2 in 1 / 15M PB, 0.3M NaCl, pH=7.4 solution for 2h, 25°C. Then wash the electrode with 1 / 15M PB, 0.1M NaCl, pH=7.4 solution for three times and store it at 4°C for future use.

Embodiment 3

[0041] Example 3: Construction of Hg when the whole thymine (T) oligonucleotide chain assembled on the surface of the gold electrode is at high density 2+ Detection of electrochemical sensors.

[0042] Soak an unmodified clean gold electrode in 100mM PB containing 1μM probe 1 that has been reduced with tris[2-carboxyethyl]phosphine (TCEP) for 1h, 1.5M NaCl, pH=7.2, and place at room temperature, Self-assemble overnight. The assembled electrode was rinsed twice with 10mM Tris, 0.5M NaCl, pH = 8.0, then placed in an aqueous solution containing 1mM mercaptohexanol (MCH) to block for 30 minutes, and then washed with 10mM Tris, 0.5M NaCl, pH = 8.0 Wash three times, then react with 500nM probe 2 in 1 / 15M PB, 0.3M NaCl, pH=7.4 solution for 2h, 25°C. Then wash the electrode three times with 1 / 15M PB, 0.1M NaCl, pH=7.4 solution, and store it at 4°C for future use.

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Abstract

The invention relates to an electrochemical sensor for mercury ion detection, and a manufacturing method and a detection method thereof and belongs to the technical field of bioanalysis. The electrochemical sensor is a gold electrode, wherein the gold electrode comprises a full-thymine (T) oligonucleotide chain which is fixed on the surface of the gold electrode and is subjected to chemical modification, and a ferrocene-marked full-adenine (A) complementary chain hybridized with the full-thymine (T) oligonucleotide chain. The Hg<2+> amount which can be contained by the sensor is be adjusted, so that the dynamic range of the sensor is directly related with the density of the full-thymine (T) oligonucleotide chain on the surface of the gold electrode. When the density of the full-thymine (T) oligonucleotide chains on the surface of the gold electrode are respectively low, medium and high, the corresponding dynamic ranges of the sensor are sequentially 10pM-100nM, 10pM-100uM and 1uM-1mM. The electrochemical sensor assembled by uisng the method has high ion selectivity and can not be interfered by other common metal ions.

Description

technical field [0001] The invention relates to an electrochemical sensor for mercury ion detection, a manufacturing method and a detection method, and belongs to the technical field of biological analysis. Background technique [0002] The dynamic range of an analytical detection method is an extremely important parameter in quantitative analysis. Sensors with a wide kinetic range are especially desirable for on-site detection, where the analyte may exist over a large and unknown concentration range. A large number of studies tend to focus more on improving the sensitivity of the detection method, but few studies on improving the detection kinetic interval. Mercury is a highly toxic global environmental pollutant. Even a very small amount exists in the environment, which poses a great threat to the health of animals, plants and humans. Water-soluble divalent mercury ion (Hg 2+ ) is one of the most common and stable forms of mercury pollution. At present, the traditional...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 娄新徽赵滔
Owner CAPITAL NORMAL UNIVERSITY
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