Method for detecting pesticide residues through paper-based visualized molecular imprinting biosensor

A biosensor and molecular imprinting technology, applied in the field of quantitative analysis and detection of pesticide residues, can solve the problems of unfavorable on-site detection, limited application and development, and expensive instruments, so as to get rid of the limitation of energy requirements, facilitate popularization and application, and reduce detection costs Effect

Inactive Publication Date: 2015-12-30
UNIV OF JINAN
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Problems solved by technology

Gas chromatography-mass spectrometry is the most widely used method for pesticide residue analysis. Gas chromatography-mass spectrometry is used for pesticide residue analysis. However, traditional gas chromatography-mass spectrometry and other pesticide residue analysis techniques are expensive and time-consuming, which poses a challenge to the food safety supervision department. The pre-production, mid-production and post-production supervision of agricultural products has brought a lot of

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  • Method for detecting pesticide residues through paper-based visualized molecular imprinting biosensor
  • Method for detecting pesticide residues through paper-based visualized molecular imprinting biosensor

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[0034] Example 1: Detection of Glucose in Serum

[0035] (1) Use Adobe IllustratorCS4 software to design the microfluidic paper chip on the computer, the style is as attached figure 1 As shown, the microfluidic paper chip includes two wax-printed hydrophobic regions A and B, respectively, and the size of a single unit is 15 × 15 mm. A hydrophilic region is used for in-situ synthesis of molecularly imprinted polymers, and B hydrophilic region is used for in situ synthesis of molecularly imprinted polymers. fixed 3,3 ’ ,5,5 ’ - tetramethylbenzidine, the diameter of the hydrophilic region is 6 mm;

[0036] (2) To prepare and synthesize zinc ferrite, first synthesize ZnO, add 80 mL of secondary water to a three-necked flask and heat to 80 °C, and then add 0.8 mmol of ZnCl 2 and 4 mmol of NaBH 4 Stir for 2h, wash by centrifugation, and dry at 60°C for later use; zinc ferrite synthesis: 0.097g FeCl 3 , 0.053g of ascorbic acid and 0.024g of ZnO prepared above were mixed and adde...

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Abstract

The invention provides a method for detecting pesticide residues through a paper-based visualized molecular imprinting biosensor, and discloses the visualized portable microfluidic paper chip sensor which is easy to operate and low in cost and is successfully applied to on-site detection. Through the sensor, a molecular imprinting technology is successfully introduced to a microfluidic paper chip, and color changing is triggered through a catalytic effect of zinc ferrite on hydrogen peroxide and 3,3',5,5'-tetramethyl benaidine to achieve visualized detection. The sensor is constructed through the following steps that hydrophobic patterns are printed in batches, wax melting forming is performed, and the zinc ferrite is prepared and synthesized; molecular imprinting polymers are synthesized in situ; a hydrogen peroxide solution is added to trigger a reaction, and the concentration range of the pesticide residues is determined by observing the color changes on the paper chip.

Description

technical field [0001] The present invention relates to the field of quantitative analysis and detection of pesticide residues, more specifically the preparation of a colorimetric biosensor for detecting pesticide residues based on molecular imprinting, and the invention also relates to the use of the paper-based molecular imprinting colorimetric sensor to detect pesticides in agricultural products residual method. Background technique [0002] Pesticide residue refers to the general term of pesticide parent, derivatives, metabolites, degradation products and impurities that remain in the environment, organisms and food after pesticide use. The main causes of excessive pesticide residues in vegetables are organophosphorus pesticides and carbamate pesticides that are prohibited from being used in vegetable production in some countries, such as methamidophos, omethoate, phorate, parathion, and methyl parathion wait. Eating food containing a large amount of highly toxic and h...

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Application Information

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IPC IPC(8): G01N21/78
Inventor 王衍虎李帅高超民于京华葛慎光颜梅
Owner UNIV OF JINAN
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