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Preparation method and application of electrochemiluminescence immunoassay sensor for detecting tumor marker

A technology of tumor markers and electrochemistry, which is applied in the field of detection of immune markers, can solve problems such as frequent contact between operators and immune substances, health hazards of operators, and expensive instruments, so as to facilitate on-site detection, reduce impact, The effect of enhancing electrical conductivity

Inactive Publication Date: 2015-01-21
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these immunoassay methods generally take a long time and have cumbersome operations such as adding samples, incubating, and washing, and the operators have more contact with immune substances, which may cause harm to the health of the operators.
Moreover, some detection methods with fast determination speed, high sensitivity and low detection limit require expensive instruments and cannot be popularized in developing countries.

Method used

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  • Preparation method and application of electrochemiluminescence immunoassay sensor for detecting tumor marker
  • Preparation method and application of electrochemiluminescence immunoassay sensor for detecting tumor marker
  • Preparation method and application of electrochemiluminescence immunoassay sensor for detecting tumor marker

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preparation example Construction

[0034] A method for preparing an electrochemiluminescent sensor for detecting an immune marker, comprising the following steps:

[0035] (1) Cut the conductive glass into the required size and perform pretreatment;

[0036] (2) Use existing methods to polymerize polyaniline in the ITO square area and synthesize g-C 3 N 4 , Silver-gold core-shell nanospheres and metal oxides, metal oxides include zinc oxide, copper oxide, manganese dioxide;

[0037] (3) Carboxylated g-C 3 N 4 , immobilized on polyaniline with a linker for immobilizing the primary antibody;

[0038] (4) Silver-gold core-shell nanospheres are modified on the metal oxide, and then the secondary antibody is modified on the silver-gold core-shell nanospheres;

[0039](5) Combine the prepared electrochemiluminescence sensor with an electrochemiluminescence instrument to detect tumor markers.

[0040] The polyaniline of the present invention / g-C 3 N 4 , the preparation of metal oxide / silver-gold and Konanomete...

Embodiment 1

[0053] Example 1 (embryonic antigens, such as alpha-fetoprotein, AFP)

[0054] (1) Select AFP with higher clinical incidence for determination;

[0055] (2) The conductive glass was first cut into strips of 4 cm×1 cm, followed by ultrasonic washing with ethanol, acetone and secondary water for 15 min, and a mold for vaseline printing template was made by Adobe Illustrator, and vaseline was coated on the A specific area on the ITO conductive glass;

[0056] (3) Preparation of polyaniline: The preparation of polyaniline is prepared by electropolymerization. It is carried out in a three-electrode system composed of conductive glass as the working electrode, platinum electrode as the counter electrode, and Ag / AgCl electrode as the reference electrode. Composed of 4.00 mL 3 M nitric acid and 0.40 mL aniline, the voltage applied on the working electrode is 0.1 V s -1 The scanning speed was cycled between -0.2-1.2 V, thus producing polyaniline;

[0057] (4) g-C 3 N 4 Preparati...

Embodiment 2

[0072] Example 2 (Carbohydrate markers, such as CA 125)

[0073] (1) Select CA125 with higher clinical incidence for determination;

[0074] (2) The conductive glass was first cut into strips of 4 cm×1 cm, followed by ultrasonic washing with ethanol, acetone and secondary water for 15 min, and a mold for vaseline printing template was made by Adobe Illustrator, and vaseline was coated on the A specific area on the ITO conductive glass;

[0075] (3) Preparation of polyaniline: The preparation of polyaniline is prepared by electropolymerization. It is carried out in a three-electrode system composed of conductive glass as the working electrode, platinum electrode as the counter electrode, and Ag / AgCl electrode as the reference electrode. Composed of 4.00 mL 3 M nitric acid and 0.40 mL aniline, the voltage applied on the working electrode is 0.1 V s -1 The scanning speed was cycled between -0.2-1.2 V, thus producing polyaniline;

[0076] (4) g-C 3 N 4 Preparation: Weigh 10...

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Abstract

The invention discloses an electrochemiluminescence immunoassay sensor for detecting a tumor marker. A preparation method of the sensor comprises the following steps: synthesizing polyaniline on conducting glass by an electropolymerization method, preparing g-C3N4, a silver-gold core shell nanosphere or copper oxide or manganese dioxide by the existing method, connecting the g-C3N4 on the polyaniline by 1-(3-dimethylamino propyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide mixed solution used as coupling agent, modifying the primary antibody on the prepared conducting glass, coating the surface of zinc oxide by the silver-gold core shell nanosphere, modifying second antibody on the silver-gold core shell nanosphere coating the surface of the zinc oxide, and detecting by electrochemiluminescence. The sensor has strong specificity, high sensitivity, simplicity in operation, wide linearity range and low detection limit.

Description

technical field [0001] The invention relates to the technical field of detection of immune markers, more specifically to the preparation of an electrochemiluminescence immunosensor, and also relates to a method for detecting tumor markers using the electrochemiluminescence sensor. Background technique [0002] For a long time, malignant tumors, also known as cancer, have been a serious threat to human health and life safety. Cancer cells refer to a new organism produced by malignant transformation and clonal proliferation of some cells with division potential under the action of carcinogenic factors. In addition to their own uncontrolled growth, cancer cells can also invade surrounding normal tissues and even transfer to other parts of the body through the body's circulatory system or lymphatic system, thereby causing body disease or even death. Therefore, early diagnosis and early treatment of cancer are of great significance. However, it is difficult to detect the low an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N21/76G01N27/26
CPCG01N21/66G01N21/76G01N33/574G01N33/57473
Inventor 颜梅王秀于京华申蕾邓文平葛慎光张彦刘海云杨春蕾
Owner UNIV OF JINAN
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