The invention relates to the technical field of petroleum fermentation and particularly relates to a detection sampling device for petroleum fermentation treatment. Technical problems that in the prior art, sampling is not comprehensive, and fermentation fails due to uncovering sampling are solved. The detection sampling device for petroleum fermentation treatment comprises a first rotating disc, a telescopic mechanism, a sealing mechanism, a sampling mechanism, a turnover mechanism, a fixing mechanism and a collecting mechanism, wherein the telescopic mechanism is arranged in the middle of the first rotating disc, the sealing mechanism is arranged on the right portion of the telescopic mechanism, the sampling mechanism is located below the sealing mechanism, and the turnover mechanism is arranged below the sampling mechanism; the fixing mechanism is arranged below the turnover mechanism, and the collecting mechanism is arranged below the fixing mechanism. The detection sampling device is advantaged in that omnibearing sampling of petroleum fermentation liquor in a fermentation tank is achieved through the first rotating disc and the telescopic mechanism, isolation of air inside and outside the tank body during sampling is achieved through the sealing mechanism, an upper surface of a conical cover entering the tank body does not make contact with outside air, and the collecting mechanism is simply and conveniently fixed, assembled and disassembled through the overturning mechanism and the fixing mechanism.