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Preparation method and application of electrochemical aptamer sensor for detecting dopamine (DA)

An aptamer sensor and dopamine technology, which is applied to the detection of dopamine in serum and the field of electrochemical aptamer sensors, can solve the problems of complicated instrument operation, high detection cost, long detection time, etc., and achieves simple operation, high sensitivity, Detecting fast effects

Inactive Publication Date: 2014-03-26
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These detection methods have the disadvantages of high detection cost, long detection time, complicated instrument operation, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for preparing an electrochemical aptamer biosensor for detecting dopamine (DA), the steps are as follows:

[0025] (1) Take 10 μL of amide-amine dendrimer complex to modify the surface of the gold electrode, and let it dry overnight;

[0026] (2) Adsorb nickel ferricyanide nanoparticles onto the electrode by electrostatic adsorption;

[0027] (3) Then electrodeposit a layer of gold nanoparticles;

[0028] (4) Immerse the modified electrode in the dopamine-aptamer solution and incubate for 10 h;

[0029] (5) Wash the above electrode with PBS buffer, then take 10 μL of dopamine solution to modify the electrode, and incubate for 30 min;

[0030] (6) Scan the cyclic voltammogram of the electrode (5) in the buffer solution of PBS 7.2, and record the change of the current signal. Set parameters: scan voltage: -0.2 to 0.6 V, scan rate: 50 mV / s, number of scan turns: 3 turns.

Embodiment 2

[0032] A method for preparing an electrochemical aptamer biosensor for detecting dopamine (DA), the steps are as follows:

[0033] (1) Take 10 μL of amide-amine dendrimer complex to modify the surface of the gold electrode, and let it dry overnight;

[0034] (2) Adsorb nickel ferricyanide nanoparticles onto the electrode by electrostatic adsorption;

[0035] (3) Then electrodeposit a layer of gold nanoparticles;

[0036] (4) Immerse the modified electrode in the dopamine-aptamer solution and incubate for 10 h;

[0037] (5) Wash the above electrode with PBS buffer, then take 10 μL of dopamine solution to modify the electrode, and incubate for 60 min;

[0038] (6) Scan the cyclic voltammogram of the electrode (5) in the buffer solution of PBS 7.2, and record the change of the current signal. Set parameters: scan voltage: -0.2 to 0.6 V, scan rate: 50 mV / s, number of scan turns: 3 turns.

Embodiment 3

[0040] A method for preparing an electrochemical aptamer biosensor for detecting dopamine (DA), the steps are as follows:

[0041] (1) Take 10 μL of amide-amine dendrimer complex to modify the surface of the gold electrode, and let it dry overnight;

[0042] (2) Adsorb nickel ferricyanide nanoparticles onto the electrode by electrostatic adsorption;

[0043] (3) Then electrodeposit a layer of gold nanoparticles;

[0044] (4) Immerse the modified electrode in the dopamine-aptamer solution and incubate for 10 h;

[0045] (5) Wash the above electrode with PBS buffer, then take 10 μL of dopamine solution to modify the electrode, and incubate for 90 min;

[0046] (6) Scan the cyclic voltammogram of the electrode (5) in the buffer solution of PBS 7.2, and record the change of the current signal. Setting parameters: scanning voltage: -0.2 to 0.6V, scanning rate: 50 mV / s, number of scanning circles: 3 circles.

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Abstract

The invention provides an electrochemical aptamer sensor for detecting dopamine (DA). The preparation method of the electrochemical aptamer sensor comprises the following steps: modifying the prepared polyamidoamine dendrimer-gold nanoparticles (PAMAM-GNPs) compound and nickel hexacyanoferrate nanoparticles (NiHCFNPs) to the electrode surface by utilizing the electrode surface modification technology, then carrying out electro-deposition on one layer of gold nanoparticles, modifying on the electrode surface, modifying amino group to the tail end of the DA connected with an aptamer, modifying the amino group on the electrode surface by affinity of the gold nanoparticles and the amino group, and finally, combining one layer of DA to be detected outside the aptamer, thereby preparing the electrochemical aptamer sensor. The prepared electrochemical aptamer sensor used as a working electrode is connected to an electrochemical workstation, an electrochemical signal changes before and after DA connection, and the change of the electrochemical signal is utilized to detect the DA. The prepared molecularly imprinted electrochemical sensor has strong selectivity and high sensitivity, is simple and rapid to operate and is suitable for DA detection.

Description

technical field [0001] The invention belongs to the field of nanomaterials and neurotransmitter detection in serum, and provides an electrochemical aptamer sensor for detecting dopamine, which can be used for the detection of dopamine in serum. Background technique [0002] Dopamine (DA), whose chemical name is 3,4-dihydroxy-β-phenylethylamine, helps cells transmit impulses, is an important neurotransmitter widely found in the central nervous system of mammals. Abnormal concentration of dopamine can cause many diseases, such as schizophrenia, Parkinson's disease and Alzheimer's disease, etc. In addition, dopamine is also related to addictive reactions such as smoking and drug use. Therefore, the analysis and determination of dopamine has always been concerned by the fields of electroanalytical chemistry, biology and medical research, and it is of great significance to establish a simple, sensitive and rapid detection method for dopamine. At present, there are many methods f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/327
Inventor 黄加栋徐伟刘素其他发明人请求不公开姓名
Owner UNIV OF JINAN
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