Preparation method and application of molecular imprinting electroluminescent sensor for detecting trace veterinary drug residue by taking battery as power

A technology of molecular imprinting and veterinary drug residues, applied in the direction of electrical excitation analysis, material excitation analysis, etc., can solve the problems of low sensitivity and selectivity, expensive reagents, uncomfortable rapid detection, etc., to achieve high sensitivity and detection range, improve sensitivity and accuracy, to achieve the effect of high-throughput screening

Inactive Publication Date: 2013-05-22
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007]2. The immunological method has high sensitivity, but the operation steps are relatively complicated, but the reagents are expensive and the cost is relatively high
[0008]3. HPLC detection of veterinary drug residues is currently used by most domestic testing institutions. Although the sensitivity is high, the pretreatment of samples is relatively Disadvantages such as complexity, long detection cycle, complicated procedures, and various reagents required
[0009]4. The UV-Vis spectrophotometry method has the advantages of simple operation, low cost, and wide linear range, but its sensitivity and selectivity are not high, so the concentration used is It is limited to 10-

Method used

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  • Preparation method and application of molecular imprinting electroluminescent sensor for detecting trace veterinary drug residue by taking battery as power
  • Preparation method and application of molecular imprinting electroluminescent sensor for detecting trace veterinary drug residue by taking battery as power
  • Preparation method and application of molecular imprinting electroluminescent sensor for detecting trace veterinary drug residue by taking battery as power

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 (Antibiotics such as chloramphenicol)

[0047]A method for preparing a battery-powered molecularly imprinted electroluminescence sensor for detecting chloramphenicol, comprising the following steps:

[0048] (1) Select the functional monomer diethylaminoethyl methacrylate (DEAEM) that can synthesize MIPs with chloramphenicol;

[0049] (2) Template molecule chloramphenicol, functional monomer diethylaminoethyl methacrylate (DEAEM), cross-linking agent ethylene glycol dimethacrylate (EGDMA), porogen methanol, initiator azobisiso Butyronitrile (ABIN), mixed uniformly in a molar ratio of 0.1:1:5:40:0.05 to obtain chloramphenicol MIPs sol;

[0050] (3) Preparation of graphene oxide

[0051] Add 2 g of graphite powder into an ice-water bath containing 2 g of sodium nitrate and 40 mL of concentrated sulfuric acid (98%), and stir magnetically for 20 min. Under stirring, 6 g of potassium permanganate was added, and stirred at room temperature for 1 h. Add 180 mL ...

Embodiment 2

[0064] Example 2 (beta-adrenergics such as ractopamine)

[0065] A method for preparing a battery-powered molecularly imprinted electroluminescent sensor for detecting ractopamine, comprising the following steps:

[0066] (1) Select the functional monomer α-methacrylic acid (MAA) that can synthesize MIPs with ractopamine;

[0067] (2) Template molecule ractopamine, functional monomer α-methacrylic acid (MAA), cross-linking agent ethylene glycol dimethacrylate (EGDMA), porogen methanol, initiator azobisisobutyronitrile (ABIN ), mixed uniformly at a molar ratio of 0.1:1:2:35:0.15 to obtain ractopamine MIPs sol;

[0068] (3) Preparation of graphene oxide

[0069] Add 2 g of graphite powder into an ice-water bath containing 2 g of sodium nitrate and 40 mL of concentrated sulfuric acid (98%), and stir magnetically for 20 min. Under stirring, 6 g of potassium permanganate was added, and stirred at room temperature for 1 h. Add 180 mL of ultrapure water to the above solution, ...

Embodiment 3

[0082] Example 3 (sedatives such as barbiturates)

[0083] A method for preparing a battery-powered molecularly imprinted electroluminescent sensor for detecting barbiturates, comprising the following steps:

[0084] (1) Select the functional monomer α-methacrylic acid (MAA) that can synthesize MIPs with barbiturates;

[0085] (2) Template molecule barbiturate, functional monomer α-methacrylic acid (MAA), cross-linking agent ethylene glycol dimethacrylate (EGDMA), porogen chloroform, initiator azobisisobutyronitrile ( ABIN), mixed uniformly in a molar ratio of 0.5:1:6:40:0.1 to obtain barbiturate MIPs sol;

[0086] (3) Preparation of graphene oxide

[0087] Add 2 g of graphite powder into an ice-water bath containing 2 g of sodium nitrate and 40 mL of concentrated sulfuric acid (98%), and stir magnetically for 20 min. Under stirring, 6 g of potassium permanganate was added, and stirred at room temperature for 1 h. Add 180 mL of ultrapure water to the above solution, and...

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PUM

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Abstract

The invention discloses a molecular imprinting electroluminescent sensor for detecting veterinary drug residue by taking a battery as power and a method for detecting veterinary drug residue. A preparation method of an electrode (a schematic diagram is shown in the figure) comprises the following steps of: preparing MIP (Molecularly Imprinted Polymer)s sol of the veterinary drug residue; preparing a carbon dot and preparing a graphene nano material according to documents; and modifying graphene, the carbon dot and the MIPs sol onto the surface of the electrode of the sensor. The method for detecting the trace veterinary drug residue comprises the following steps of: connecting the modified electrode to an electrogenerated chemiluminescence instrument, and detecting the veterinary drug residue in a sample extract by taking the battery as the power. The molecular imprinting electroluminescent sensor disclosed by the invention has the advantages that the specificity of the electrode is strong and the sensitivity is high and can be up to a ng grade; only 3-5 minutes are spent on a basic detection process; and the cost is low. The method for detecting the veterinary drug residue by adopting the electrode is quick and easy to operate, the reaction is automatically completed by instruments and results are automatically recorded by the instruments.

Description

technical field [0001] The present invention relates to the technical field of detection of trace veterinary drug residues, and more specifically to the preparation of a battery-powered molecularly imprinted electroluminescent sensor applied to the detection of trace veterinary drug residues. The present invention also relates to the use of the described Molecularly imprinted electrochemiluminescence sensor for detection of veterinary drug residues in livestock, poultry meat and dairy products. Background technique [0002] It is customary to refer to drugs and their products that are applied to various animals to prevent, treat, maintain, diagnose diseases or improve animal production performance as veterinary drugs. Veterinary drug residues refer to drug prototypes, metabolites and drug impurities that are accumulated or stored in animal cells, tissues or organs after drugs are administered to animals. The 32nd meeting of the WHO Joint Expert Committee on Food Additives (...

Claims

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Application Information

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IPC IPC(8): G01N21/66
Inventor 于京华张彦葛磊葛慎光颜梅黄加栋王少伟苏敏刘伟艳刘芳李蒙楚成超李龙
Owner UNIV OF JINAN
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