Preparation method and application of molecular imprinting electroluminescent sensor for detecting trace veterinary drug residue by taking battery as power

Abstract

The invention discloses a molecular imprinting electroluminescent sensor for detecting veterinary drug residue by taking a battery as power and a method for detecting veterinary drug residue. A preparation method of an electrode (a schematic diagram is shown in the figure) comprises the following steps of: preparing MIP (Molecularly Imprinted Polymer)s sol of the veterinary drug residue; preparing a carbon dot and preparing a graphene nano material according to documents; and modifying graphene, the carbon dot and the MIPs sol onto the surface of the electrode of the sensor. The method for detecting the trace veterinary drug residue comprises the following steps of: connecting the modified electrode to an electrogenerated chemiluminescence instrument, and detecting the veterinary drug residue in a sample extract by taking the battery as the power. The molecular imprinting electroluminescent sensor disclosed by the invention has the advantages that the specificity of the electrode is strong and the sensitivity is high and can be up to a ng grade; only 3-5 minutes are spent on a basic detection process; and the cost is low. The method for detecting the veterinary drug residue by adopting the electrode is quick and easy to operate, the reaction is automatically completed by instruments and results are automatically recorded by the instruments.

Description

technical field [0001] The present invention relates to the technical field of detection of trace veterinary drug residues, and more specifically to the preparation of a battery-powered molecularly imprinted electroluminescent sensor applied to the detection of trace veterinary drug residues. The present invention also relates to the use of the described Molecularly imprinted electrochemiluminescence sensor for detection of veterinary drug residues in livestock, poultry meat and dairy products. Background technique [0002] It is customary to refer to drugs and their products that are applied to various animals to prevent, treat, maintain, diagnose diseases or improve animal production performance as veterinary drugs. Veterinary drug residues refer to drug prototypes, metabolites and drug impurities that are accumulated or stored in animal cells, tissues or organs after drugs are administered to animals. The 32nd meeting of the WHO Joint Expert Committee on Food Additives (...

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Problems solved by technology

[0007]2. The immunological method has high sensitivity, but the operation steps are relatively complicated, but the reagents are expensive and the cost is relatively high

[0008]3. HPLC detection of veterinary drug residues is currently used by most domestic testing institutions. Although the sensitivity is high, the pretreatment of samples is relatively Disadvantages such as complexity, long detection cycle, complicated procedures, and various reagents required

[0009]4. The UV-Vis spectrophotometry method has the advantages of simple operation, low cost, and wide linear range, but its sensitivity and selectivity are not high, so the concentration used is It is limited to 10-6 levels, so when using a UV detector to determine antibiotic residue samples, it generally needs to be concentrated to meet the requirements

[0010]5. Thin-layer chromatography can detect penicillin residues in animal tissues, but its disadvantages are complex, high operating costs, long time and low sensitivity

[0011] For the detection and analysis of veterinary drug residues, the above methods generally have high detection cost, false positive results, complicated detection process, high cost, large amount of reagents, discomfort Due to the disadvantages of quick detection on site and large interference of coexisting substances, it cannot meet the needs of actual detection.

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