Sanitary status on-site rapid detection device and detecting method

Abstract

The invention discloses a sanitary quality locale rapid detection device and a detection method, the device comprises an auto sampling element, a photoelectricity conversion element and a control, operation and storage display circuit element. The method comprises that: the detected parameters are selected, based on an ATP bioluminescence theory, the detected sample and three groups detection reagents of a body cell lysates agent, a bacterial cell lysates agent and a luciferase-luciferase luminescence reagent are added to a sample pool, each reagent is provided with an auto sample adding device connected with the sample pool, the auto sample adding device controls to inject different reagents to the sample pool according to the detection requirement, the sample surface cleanliness condition is gained by measuring the total ATP content, the bacteria quantity in the sample is quantitatively detected by measuring the ATP content in the bacteria cell. Compared with the traditional device, the detection speed of the invention is fast, the detection time is less than 30 minutes, the degree of automation is high, and the maneuverability is good, the invention can be applicable to the locale rapid detection of health quality in the food, cosmetics, medical, environmental, and other fields.

Description

technical field [0001] The invention belongs to the technical field of sanitation and epidemic prevention, and relates to optical-mechanical-electrical integration technology and bioluminescence technology, and in particular to on-site rapid detection of sanitation conditions. Background technique [0002] With the increasing demand for the control and detection of bacteria or microorganisms in public health services such as cosmetics, food, and pharmaceuticals, the requirements for related inspection methods and detection devices are getting higher and higher. At present, the quality inspection and quarantine department generally adopts the traditional culture method for the detection of the total number of bacteria and microbial indicators. The specific sampling method, culture method and measurement method are all specified in the national standard (GB / T 4789.2-2003). The advantage of this method is high accuracy, and the disadvantage is that the detection time is long, t...

AI Technical Summary

Problems solved by technology

[0006] 1. Since the detection process itself requires a variety of detection reagents, the current addition to the device is mostly done manually, the steps are cumbersome, and the degree of automation is not enough. Professionals are required to operate, and many accessories are required, including pipettes, kits, etc. Facilitate on-site inspection;

[0007] 2. The application area is relatively narrow, and generally only one of the parameters of the total number of bacteria or surface cleanliness is tested

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