Sanitary status on-site rapid detection device and detecting method

A detection device and detection method technology, applied in the field of sanitation and epidemic prevention, can solve the problems of insufficient automation, unfavorable on-site detection, cumbersome steps, etc., and achieve the effects of good operability, short detection time, and high degree of automation

Active Publication Date: 2008-01-30
INST OF ELECTRONICS CHINESE ACAD OF SCI
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Problems solved by technology

[0006] 1. Since the detection process itself requires a variety of detection reagents, the current addition to the device is mostly done manually, the steps are cumbersome, and the degree of automation is not enough. Professionals are required to operate,

Method used

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  • Sanitary status on-site rapid detection device and detecting method
  • Sanitary status on-site rapid detection device and detecting method
  • Sanitary status on-site rapid detection device and detecting method

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specific Embodiment

[0036] The automatic sampling unit 1 includes three reagent bottles 4, 5, 6, three independent automatic sampling parts 7, 8, 9 and a sample pool 10; three independent automatic sampling parts 7, 8, 9 Connect with reagent bottle 4,5,6 and sample cell 10 respectively, control unit 3 is connected with automatic sampling part 7,8,9, is used for the detection reagent in reagent bottle 4,5,6 by reagent bottle 4,5 , 6 suck out and inject into the sample pool 10, and the detection reagent reacts with the tested sample in the sample pool 10. Among them, reagent bottles 4, 5, and 6 are 4mL general-purpose brown reagent bottles to prevent the influence of external light on the luminescent reagents. The automatic sampling parts 7, 8, and 9 can be selected as miniature peristaltic pumps, which are small in size, about 6×5×3cm, which is convenient for integration in small instruments; the speed range is 0.1-50rpm, and the flow range is 0.006-37mL / min , suitable for precise control of the ...

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Abstract

The invention discloses a sanitary quality locale rapid detection device and a detection method, the device comprises an auto sampling element, a photoelectricity conversion element and a control, operation and storage display circuit element. The method comprises that: the detected parameters are selected, based on an ATP bioluminescence theory, the detected sample and three groups detection reagents of a body cell lysates agent, a bacterial cell lysates agent and a luciferase-luciferase luminescence reagent are added to a sample pool, each reagent is provided with an auto sample adding device connected with the sample pool, the auto sample adding device controls to inject different reagents to the sample pool according to the detection requirement, the sample surface cleanliness condition is gained by measuring the total ATP content, the bacteria quantity in the sample is quantitatively detected by measuring the ATP content in the bacteria cell. Compared with the traditional device, the detection speed of the invention is fast, the detection time is less than 30 minutes, the degree of automation is high, and the maneuverability is good, the invention can be applicable to the locale rapid detection of health quality in the food, cosmetics, medical, environmental, and other fields.

Description

technical field [0001] The invention belongs to the technical field of sanitation and epidemic prevention, and relates to optical-mechanical-electrical integration technology and bioluminescence technology, and in particular to on-site rapid detection of sanitation conditions. Background technique [0002] With the increasing demand for the control and detection of bacteria or microorganisms in public health services such as cosmetics, food, and pharmaceuticals, the requirements for related inspection methods and detection devices are getting higher and higher. At present, the quality inspection and quarantine department generally adopts the traditional culture method for the detection of the total number of bacteria and microbial indicators. The specific sampling method, culture method and measurement method are all specified in the national standard (GB / T 4789.2-2003). The advantage of this method is high accuracy, and the disadvantage is that the detection time is long, t...

Claims

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Application Information

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IPC IPC(8): G01N35/00G01N21/76
Inventor 岳伟伟蔡新霞何保山周爱玉罗金平刘剑
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
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