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Rapid detecting method of sulphate reducing bacteria and kit thereof

A detection method, the sulfate technology, is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions. It can solve the problems of long time consumption, low accuracy and sensitivity, and achieve shortening. The effect of detection time, strong specificity, and convenience for on-site detection

Active Publication Date: 2014-08-13
CHINA PETROLEUM & CHEM CORP +1
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Problems solved by technology

[0004] Aiming at the problems of long time consumption and low detection accuracy and sensitivity of the SRB detection method in the prior art, the present invention provides a rapid detection method for sulfate-reducing bacteria and a kit thereof, so as to shorten the time for sulfate reduction in oil field produced water samples. Bacteria detection cycle, and improve detection accuracy and sensitivity

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  • Rapid detecting method of sulphate reducing bacteria and kit thereof
  • Rapid detecting method of sulphate reducing bacteria and kit thereof
  • Rapid detecting method of sulphate reducing bacteria and kit thereof

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Embodiment Construction

[0034] The kit and its test method of the present invention will be described in detail below with specific examples.

[0035]Before testing the water sample, get the known solution that APS content is respectively 100 / ml, 1000 / ml, 10000 / ml, 100000 / ml and 1000000 / ml, by the method of the present invention through reaction substrate catalysis and After the reaction of the chromogenic agent, the color displayed by the solution is detected by a chemiluminescence instrument for its OD value, and then made as figure 1 The shown SRB content rapid detection standard curve and figure 2 Standard color chart shown.

[0036] The water samples to be tested are tested by the following methods:

[0037] (1) Take 15ml of the oil field production water sample to be tested, filter it with a 200-mesh sieve and place a beaker under the sieve to accept the filtered water sample, wash the sieve and the impurity particles measured on the net with sterile ultrapure water 1- 2 times, collect the...

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Abstract

The invention relates to the technical field of detecting and processing of oilfield water samples, and in particular relates to a rapid detecting method of sulphate reducing bacteria and a kit for detecting. The rapid detecting method disclosed by the invention comprises the following steps: firstly, pre-processing and removing impurities in a water sample to be tested, then adding a bacteria lysis solution into the water sample to sufficiently split and release APS thallus in the water sample, adding a reaction substrate, catalyzing the reaction substrate by using enzyme to carry out oxidization-reduction reaction, adding a colour developing substrate into the reaction system, combining the reaction product catalyzed by enzyme with the colour developing substrate, and thus obtaining a coloured reaction end-product. The content of SRB (Sulphate Reducing Bacteria) in the water sample to be tested is obtained according to reaction colours. According to the invention, a device for detecting SRB and a reagent are standardized to form a kit according to the detection method, so that various construction fields are convenient to detect; the rapid detecting method of sulphate reducing bacteria and the kit disclosed by the invention are capable of effectively eliminating impurity interference in the oilfield water sample, greatly shortening the detection period and greatly increasing the detection accuracy and sensibility.

Description

technical field [0001] The invention relates to the technical field of oilfield water sample detection and processing, in particular to a rapid detection method for sulfate-reducing bacteria and a detection kit. Background technique [0002] Sulfate-reducing bacteria (SRB) widely exist in anoxic environments such as oil wells and sewage in oil fields. Under anoxic or anaerobic conditions, it can use the organic matter attached to the metal surface as a carbon source, and use the hydrogen produced in the bacterial biofilm to reduce sulfate to hydrogen sulfide, which has a strong corrosion effect on metal equipment, causing serious loss. [0003] The currently used test bottle method is the most commonly used SRB detection method at home and abroad. This method is relatively simple, but it also has some shortcomings, the most important of which is that it takes too long, generally takes 7 days, and the detection accuracy and sensitivity are not high. Therefore, a large numb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 杨帆王彪孟章进林晶晶姚峰吴伟林
Owner CHINA PETROLEUM & CHEM CORP
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