Polymer/gold nano particle composite medium for use in capillary electrophoresis DNA sequencing and method for preparing same

A gold nanoparticle and DNA sequencing technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as low viscosity and inability to sequence DNA, reduce the possibility of polymer degradation, and facilitate injection and flushing out the capillary, the effect of viscosity reduction
CN1966719AInactive Publication Date: 2007-05-23UNIV OF SCI & TECH OF CHINA
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
UNIV OF SCI & TECH OF CHINA
Publication Date
2007-05-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to polymers / gold nanoparticles complex medium for DNA sequencing with capillary electrophoresis and its preparation method, and belongs to the biotechnological field of DNA sequencing and separation. The concentration of the buffer solution of polymers / gold nanoparticles complex medium used for DNA sequencing is 2.0%-3.0%. The invention comprises the steps of: (1) synthesis of quasi-interpenetrating polymer networks with self-spreading ability, (2) preparation of Au nanoparticles, and (3) synthesis of complex medium of quasi-interpenetrating polymer networks / gold nanoparticles. The complex medium with reduced viscosity at proper concentration is easy to be pumped into and run out of the capillaries, and can prevent the production of electroosmotic flow because of the existence of complex with self spreading ability, which saves the step of spreading inner wall of the capillary, simplifies the preparation process of column, reduces the application cost of capillary, elongates the service time of capillary. The invention is mainly used for separating DNA with capillary electrophoresis and DNA sequencing.
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Description

technical field

[0001] The invention relates to a polymer / gold nanoparticle composite medium for capillary electrophoresis DNA sequencing and a preparation method thereof, belonging to the technical field of DNA sequencing and separation biotechnology. Background technique

[0002] Capillary electrophoresis is a well-established method for DNA analysis. For capillary electrophoresis, since the separation medium determines the migration behavior and separation degree of DNA fragments, it is an important factor for DNA separation. Currently, synthetic polymers are the most widely used separation medium for DNA separation. For an effective For separation media, it should have high screening capacity, low viscosity and self-coating function at the same time.

[0003] Synthetic polymers commonly used to separate DNA mainly include hydrophilic homopolymers (Heller C., Electrophoresis, 2001, 22: 629-643). For these homopolymers, most of them need to coat the inner wall of the capi...

Claims

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