Nucleic acid aptamer probe-based rapid one-step method non-mark type bisphenol-A colorimetric detection method

Abstract

The invention discloses a nucleic acid aptamer probe-based rapid, super sensitive on-site one-step method non-mark type bisphenol-A colorimetric detection method. The detection method comprises the following steps of: (1) determining the optimal salt concentration of a detection system; (2) combining gold nano particles with a nucleic acid aptamer probe, and preparing a nucleic acid aptamer-gold nano particle composite probe; and (3) detecting the bisphenol-A. The detection method can be used for on-site, rapid and super sensitive detection without other auxiliary instruments or equipment. The detection can be accomplished within 10-15 minutes, and a qualitative and quantitative detection result can be obtained; corresponding detection of other target objects can be realized by only changing and replacing the sequence of the nucleic acid aptamer probe of the gold nano particle; and the method further has the advantages of high specificity, good stability, wide application range, low cost and the like, and therefore the method is easy to be popularized and applied to normal use.

Description

technical field [0001] The invention belongs to the technical fields of food safety, inspection and quarantine, and environmental detection, and in particular relates to a non-labeled, one-step colorimetric detection method of non-labeled bisphenol A. technical background [0002] With the development of society and the further deepening of economic globalization, the ever-increasing emerging products pose new challenges to inspection and quarantine. Although relying on traditional instruments for detection has the advantages of high detection sensitivity and good test result stability, large-scale instruments are usually expensive and require professional operators with relevant knowledge. At the same time, there are disadvantages such as high detection costs, which limit the large-scale instruments Scale detection and on-site rapid detection. The enzyme-linked immunosorbent assay based on immunoassay can solve the shortcomings of high detection cost of large-scale instrum...

AI Technical Summary

Problems solved by technology

Although relying on traditional instruments for detection has the advantages of high detection sensitivity and good test result stability, large-scale instruments are usually expensive and require professional operators with relevant knowledge. At the same time, there are disadvantages such as high detection costs, which limit the large-scale instruments Scale detection and on-site rapid detection

The enzyme-linked immunosorbent assay based on immunoassay can solve the shortcomings of high detection cost of large-scale instruments and cannot be used for on-site detection, but because the enzyme-linked immunosorbent assay requires multiple steps of repeated washing and separation processes in the detection process, It requires people with certain professional knowledge to operate, which also limits the widespread use of this method to a certain extent

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