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Method for improving meat performance of beef cattle by using EDG1 gene

A gene and beef cattle technology, applied in the field of molecular biology, can solve the problems of poor fat deposition, slow growth rate of Qinchuan cattle, and low meat production, and achieve the effect of improving meat production performance.

Pending Publication Date: 2022-01-04
INNER MONGOLIA UNIVERSITY +3
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

However, compared with foreign high-quality beef cattle breeds, Qinchuan cattle have problems such as slow growth, low meat production and poor fat deposition.

Method used

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  • Method for improving meat performance of beef cattle by using EDG1 gene
  • Method for improving meat performance of beef cattle by using EDG1 gene
  • Method for improving meat performance of beef cattle by using EDG1 gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Obtaining SNP Detection Fragments of Qinchuan Cattle and Chinese Simmental Cattle EDG1 Gene and Establishment of Polymorphism Site Detection Method

[0028] 1. Extraction of Genomic DNA from Qinchuan Cattle and Chinese Simmental Cattle

[0029] The test cattle of the present invention are 350 Qinchuan adult cows (age 18-24 months old, at least three generations unrelated) of the National Beef Cattle Improvement Center of Northwest Agriculture and Forestry University, and 260 Chinese Ximen cows of Chifeng Shengquan Ecological Animal Husbandry Co., Ltd. Tahr cattle (age 14-18 months). The genomic DNA of Qinchuan cattle and Chinese Simmental cattle was extracted using the blood genomic DNA extraction kit produced by Beijing Tiangen Biochemical Technology Co., Ltd., and the specific steps refer to the kit instructions. Evaluate the quality and quantity of the extracted DNA, evaluate it by agarose gel electrophoresis, and store it at -40°C for future use.

[0030...

Embodiment 2

[0044] Example 2 Detection of polymorphic distribution of molecular markers in Qinchuan cattle, Chinese Simmental cattle and other local beef cattle breeds

[0045] The genetic diversity of EDG1 gene g.42041062G>T site was detected in 7 cattle populations, and the detection results are shown in Table 3.

[0046] Table 3 The genotype frequency and allele frequency of EDG1 gene g.42041062G>T locus in cattle population

[0047]

[0048] Note: QC, Qinchuan cattle; LX, Luxi cattle; MGC, Mongolian cattle group (Inner Mongolia Autonomous Region, China); MGG, Mongolian cattle group (Mongolia); WL, Wuling cattle; LL, Longlin cattle; CS, China Simmental.

[0049] From the results in Table 3, it can be seen that the genotype frequencies of the g.42041062G>T site of the Chinese Qinchuan cattle EDG1 gene are: TT (0.034), GT (0.237), GG (0.729), and the G allele frequency (0.847) is higher than T allele frequency (0.153); Chinese Luxi yellow cattle, Chinese Inner Mongolia Mongolian cat...

Embodiment 3

[0050] Example 3 Investigation of the relationship between g.42041062G>T molecular markers and meat performance of Qinchuan cattle and Chinese Simmental cattle

[0051] In order to determine whether the g.42041062G>T site of the EDG1 gene is related to the difference in meat performance between Qinchuan cattle and Chinese Simmental cattle, the method established in Example 1 was used for polymorphism detection, and SPSS 19.0 software was used to analyze c.*188G >The correlation between the three genotypes of A site and the meat performance of Qinchuan cattle and Chinese Simmental cattle.

[0052] The mathematical model adopted is: Y ijklm =μ+G i +A j +F k +S l +S m +e ijklm ;

[0053] Among them, Y ijklm is the observed value of the trait, μ is the average value of the trait, G i is the genotype effect, A j is the fixed effect caused by age, F k is the pasture environmental effect, S l is the gender effect, S m is the family effect, e ijklm is a random error.

...

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Abstract

The invention belongs to the technical field of molecular biology, and particularly relates to a method for improving meat performance of beef cattle by using a g.42041062G>T site of an EDG1 gene. The method for improving the meat performance of the beef cattle by using the g.42041062G>T site of the EDG1 gene comprises the following steps of detecting whether mutation of the g.42041062G>T site exists in the EDG1 gene in a beef cattle genome to be detected: selecting a beef cattle individual with the genotype of TT, and carrying out artificial insemination and native mating. A molecular marker is used as a reliable marker for meat performance of Qinchuan cattle and Chinese Simmental cattle, and a technical system for detecting the genotype through DNA is established, so that the selection intensity and the breeding accuracy and efficiency are improved, and the meat performance of the Qinchuan cattle and the Chinese Simmental cattle is improved.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a method for improving the meat production performance of beef cattle by utilizing the g.42041062G>T site of EDG1 gene. Background technique [0002] Qinchuan cattle are named after the "Eight Hundred Li Qinchuan" located in Guanzhong, Shaanxi. Appropriate pattern and other characteristics. Qinchuan cattle breeding has become a pillar industry for farmers in Shaanxi, Gansu, Ningxia and other provinces to increase their income. However, compared with foreign high-quality beef cattle breeds, Qinchuan cattle have problems such as slow growth, low meat production and poor fat deposition. [0003] Simmental cattle are native to Switzerland. In 1990, the cattle and sheep breeding base of Shandong Provincial Animal Husbandry Bureau introduced this breed. Simmental cattle are not purebred beef cattle, but due to their high milk production, the meat production ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q1/683C12Q2600/124C12Q2600/156C12Q2531/113
Inventor 佟彬郝琦成功昝林森李庆东殷志成
Owner INNER MONGOLIA UNIVERSITY
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