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Method for improving meat production performance of beef cattle by using AKIRIN2 gene

A gene and beef cattle technology, applied in the field of molecular biology, can solve the problems of poor fat deposition, low meat production, slow growth of Qinchuan cattle, etc., and achieve the effect of improving meat production performance

Pending Publication Date: 2021-12-31
INNER MONGOLIA UNIVERSITY +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared with foreign high-quality beef cattle breeds, Qinchuan cattle have problems such as slow growth, low meat production and poor fat deposition.

Method used

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  • Method for improving meat production performance of beef cattle by using AKIRIN2 gene
  • Method for improving meat production performance of beef cattle by using AKIRIN2 gene
  • Method for improving meat production performance of beef cattle by using AKIRIN2 gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Obtaining SNP Detection Fragments of Qinchuan Cattle and Chinese Simmental Cattle AKIRIN2 Gene and Establishment of Polymorphism Site Detection Method

[0028] 1. Extraction of Genomic DNA from Qinchuan Cattle and Chinese Simmental Cattle

[0029] The test cattle of the present invention are 350 Qinchuan adult cows (age 18-24 months old, at least three generations unrelated) of the National Beef Cattle Improvement Center of Northwest Agriculture and Forestry University, and 260 Chinese Ximen cows of Chifeng Shengquan Ecological Animal Husbandry Co., Ltd. Tahr cattle (age 14-18 months). The genomic DNA of Qinchuan cattle and Chinese Simmental cattle was extracted using the blood genomic DNA extraction kit produced by Beijing Tiangen Biochemical Technology Co., Ltd., and the specific steps refer to the kit instructions. Evaluate the quality and quantity of the extracted DNA, evaluate it by agarose gel electrophoresis, and store it at -40°C for future use.

[0...

Embodiment 2

[0047] Example 2 Detection of polymorphic distribution of molecular markers in Qinchuan cattle, Chinese Simmental cattle and other local beef cattle breeds

[0048] Table 3 shows the polymorphism of the c.*188G>A site in the AKIRIN2 gene in Qinchuan cattle, Chinese Simmental cattle and other local beef cattle breeds.

[0049] Table 3 Distribution of dominant allele frequency and allele frequency of AKIRIN2 gene c.*188G>A loci in beef cattle populations in different regions

[0050]

[0051] Note: QC, Qinchuan cattle; LX, Luxi cattle; MGC, Mongolian cattle group (Inner Mongolia Autonomous Region, China); MGG, Mongolian cattle group (Mongolia); WL, Wuling cattle; LL, Longlin cattle; CS, China Simmental.

[0052] From the results in Table 3, it can be seen that there are three genotypes at the c.*188G>A locus in most regional beef cattle populations, among which the GG homozygous genotype is dominant, and the AA genotype has a low proportion. c. *188G>A site allele G is the ...

Embodiment 3c

[0053] Example 3c. Correlation analysis and application of *188G>A molecular markers with meat performance of Qinchuan cattle and Chinese Simmental cattle

[0054] In order to determine whether the c.*188G>A site is related to the difference in meat performance between Qinchuan cattle and Chinese Simmental cattle, the method established in Example 1 was used for polymorphism detection, and the c.*188G>A site was analyzed using SPSS 19.0 software Correlation of three genotypes of A locus with meat performance of Qinchuan cattle and Chinese Simmental cattle.

[0055] The mathematical model adopted is: Y ijklm =μ+G i +A j +F k +S l +S m +e ijklm ;

[0056] Among them, Y ijklm is the observed value of the trait, μ is the average value of the trait, G i is the genotype effect, A j is the fixed effect caused by age, F k is the pasture environmental effect, S l is the gender effect, S m is the family effect, e ijklm is a random error.

[0057] The association analysis ...

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Abstract

The invention belongs to the technical field of molecular biology, and particularly relates to a method for improving meat production performance of beef cattle by using a c.* 188G > A site of an AKIRIN2 gene. The method for improving the meat production performance of the beef cattle by using the c.*188G>A site of the AKIRIN2 gene comprises the following steps: detecting whether mutation of the c.*188G>A site exists in the AKIRIN2 gene in a beef cattle genome to be detected: selecting a beef cattle individual of which the genotype is AA type, and carrying out artificial insemination and natural indigo mating. According to the invention, the molecular marker is used as a reliable marker for the meat production performance of the Qinchuan cattle and the Chinese Simmental cattle, and a technical system for detecting the genotype through DNA is established, so that the selection intensity and the breeding accuracy and efficiency are improved, and the meat production performance of the Qinchuan cattle and the Chinese Simmental cattle is improved.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a method for improving the meat production performance of beef cattle by utilizing the c.*188G>A site of AKIRIN2 gene. Background technique [0002] Qinchuan cattle are named after the "Eight Hundred Li Qinchuan" located in Guanzhong, Shaanxi. Appropriate pattern and other characteristics. Qinchuan cattle breeding has become a pillar industry for farmers in Shaanxi, Gansu, Ningxia and other provinces to increase their income. However, compared with foreign high-quality beef cattle breeds, Qinchuan cattle have problems such as slow growth, low meat production and poor fat deposition. [0003] Simmental cattle are native to Switzerland. In 1990, the cattle and sheep breeding base of Shandong Provincial Animal Husbandry Bureau introduced this breed. Simmental cattle are not purebred beef cattle, but due to their high milk production, the meat production p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/683C12N15/11
CPCC12Q1/6888C12Q1/683C12Q2600/156C12Q2600/124C12Q2531/113C12Q2521/301Y02P60/87
Inventor 佟彬高一杰成功昝林森李庆东殷志成
Owner INNER MONGOLIA UNIVERSITY
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