Method for detecting fat coverage of Simmental cattle carcass by dlk1 gene marker

A Simmental, coverage technology, used in biochemical equipment and methods, recombinant DNA technology, determination/inspection of microorganisms, etc.

Active Publication Date: 2021-09-14
JILIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no report showing that the genetic marker of DLK1 gene can be used as an auxiliary selection molecular marker for screening good meat quality traits before fattening

Method used

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  • Method for detecting fat coverage of Simmental cattle carcass by dlk1 gene marker
  • Method for detecting fat coverage of Simmental cattle carcass by dlk1 gene marker
  • Method for detecting fat coverage of Simmental cattle carcass by dlk1 gene marker

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The acquisition of DLK1 gene fragments in Chinese Simmental cattle and the establishment of polymorphism detection methods in functional regions:

[0023] 1.1 Test materials: 237 28-month-old Chinese Simmental bulls came from Baolongshan beef cattle fattening farm in Tongliao City, Inner Mongolia. Jugular vein blood collection, all blood samples were 10 mL / head, anticoagulated with ACD anticoagulant, and frozen at -20°C. Genomic DNA was extracted from blood samples with a blood genomic DNA extraction kit;

[0024] 1.2 Primer design and PCR amplification: Chinese Simmental cattle were selected as test materials. In order to detect as many SNPs sites as possible, fragments with higher SNP density were selected, and the following primer pairs were designed according to the selected bovine DLK1 gene sequence:

[0025] Forward primer F: 5'-TCCACAGTGGAGGCTACTAAG-3';

[0026] Reverse primer R: 5'-CTTGTCTCCTGACTTCCTAAG-3';

[0027] Carry out PCR amplification in the Chinese ...

Embodiment 2

[0036] The DLK1 gene SNPs genetic markers obtained by screening were used to calculate the genotype frequency and gene frequency.

[0037] The C allele frequency of DLK1-478 C / T locus in Chinese Simmental cattle population is 0.865, and CC is the dominant genotype; the T allele frequency of DLK1-609 T / G locus is 0.846, and TT is the dominant genotype .

[0038]Table 1. Genetic diversity of 2 SNPs of DLK1 gene in Chinese Simmental cattle population

[0039] SNP mapping group sample allele frequency genotype frequency I3-478 C>T 237 C (0.865) T (0.135) CC (0.738) CT (0.253) TT (0.008) I3-609 T>G 217 T(0.846) G (0.154) TT (0.719) TG (0.234) GG (0.028)

Embodiment 3

[0041] Association analysis of DLK1 molecular genetic markers with Chinese Simmental beef quality and carcass traits:

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Abstract

The invention discloses a method for detecting the fat coverage rate of Simmental cattle carcass by DLK1 gene markers. Using the DNA sequence of the DLK1 gene of Chinese Simmental cattle as a template, a pair of primers are designed, and a strict PCR reaction system is carried out to carry out PCR of samples Amplification and electrophoresis, screening by sequencing and finding 2 SNP sites; the restriction endonuclease for screening DLK1-478 C / T sites in the population is Msp I, and the cleavage base limited by this enzyme is CCGG; screening The restriction endonuclease of DLK1‑609 T / G site is Ase I; the individuals carrying DLK1‑478 C / T site T allele and TT mutation genotype in Chinese Simmental cattle population have higher fat coverage and better marbling; and individuals with the G allele and GG mutant genotype carrying the DLK1‑609 T / G locus had higher fat coverage. The two mutation sites of the DLK1 gene of the present invention can be used as genetic markers predicting the fat coverage rate and marbling traits of Chinese Simmental cattle, and used for early marker-assisted selection of beef cattle.

Description

technical field [0001] The invention discloses a method for detecting the fat coverage rate of a Simmental cattle carcass with a DLK1 gene marker, which uses the DLK1 gene marker to detect the fat coverage rate and marbling traits of Chinese Simmental cattle, and belongs to the technical field of detecting beef quality traits. Background technique [0002] Chinese Simmental beef is bright red in color, fine in texture, full of elasticity, moderate in marble pattern, white or light yellow in fat color, high in hardness in fat texture, and 100% body surface fat coverage, which is difficult for ordinary beef to achieve. standard. From the perspective of genes, molecular genetic markers are used as the main means of research on beef quality traits, and quantitative traits controlled by micro-effect polygenes are screened to optimize the breeding of cattle herds. [0003] Single nucleotide polymorphisms (Single Nucleotide Polymorphisms, abbreviated as SNP) refer to the variation...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 杨润军王梦岩赵志辉房希碧余湘
Owner JILIN UNIV
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