Prevention and control method for largemouth bass ranavirus of mandarin fish

An iridescent virus and mandarin fish technology, applied in the field of aquatic animal disease prevention and control, can solve problems such as lack of control measures

Pending Publication Date: 2021-06-04
广州力必拓生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most of the disease prevention and control technologies for aquatic animals, including mandarin fish, are only satisfied with the detection and detection of related pathogens, and there is a serious lack of a series of comprehensive technologies for corresponding prevention and control measures. )” is an overall technical solution for precise prevention and control of diseases

Method used

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  • Prevention and control method for largemouth bass ranavirus of mandarin fish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] On August 19, 2020, mandarin fishes raised by a farmer in Foshan for about 50 days died, and the number of deaths per day was about 400. Later, it was detected by fluorescent quantitative PCR that the death was caused by frog iridescent virus. The following treatment plan was followed:

[0047] First stop feeding the feed.

[0048] On the 1st and 2nd day, use "Shanze No. 1 500mL / mu Shibencao Shenlu 1000mL / mu" for two consecutive days;

[0049] On the 3rd and 4th day, use "Shanduqing No. 2 40g / mu" for two consecutive days.

[0050] On the 5th and 6th days, use "povidone-iodine solution (aquaculture) 330mL / mu+herbal dew 1000mL / mu for two consecutive days.

[0051]Customer feedback: Changes in the number of deaths within 6 days after medication: 400 / 100 / dozens / several, and the death rate was finally reduced to less than a few.

Embodiment 2

[0053] On September 6, 2020, a farmer in Shunde died of mandarin fishes that had been breeding for more than 3 months, and the number of deaths was about 400 per day. Later, it was detected by fluorescent quantitative PCR that the death was caused by the frog iridescent virus, and the following treatment plan was followed. :

[0054] First stop feeding the feed.

[0055] On the 1st and 2nd day, use "Shanze No. 1 500mL / mu Shibencao Shenlu 1000mL / mu" for two consecutive days;

[0056] On the 3rd and 4th day, use "Shanduqing No. 2 40g / mu" for two consecutive days.

[0057] On the 5th and 6th days, use "povidone-iodine solution (aquaculture) 330mL / mu+herbal dew 1000mL / mu for two consecutive days.

[0058] Feedback from customers: The number of deaths within 6 days after taking the drug changed significantly: from the initial more than 400 deaths per day to a few deaths a day, the death rate finally dropped to zero.

Embodiment 3

[0060] On September 13, 2020, mandarin fishes raised by a farmer in Wuxi, Jiangsu Province for about 2 months died, and the number of deaths per day was about 300. Afterwards, it was detected by fluorescent quantitative PCR that the death was caused by the frog iridescent virus. Follow the following treatment plan deal with:

[0061] First stop feeding the feed.

[0062] On the 1st and 2nd day, use "Shanze No. 1 500mL / mu Shibencao Shenlu 1000mL / mu" for two consecutive days;

[0063] On the 3rd and 4th day, use "Shanduqing No. 2 40g / mu" for two consecutive days.

[0064] On the 5th and 6th days, use "povidone-iodine solution (aquaculture) 330mL / mu+herbal dew 1000mL / mu for two consecutive days.

[0065] Feedback from customers: The number of deaths within 6 days after taking the drug changed significantly: from more than 300 deaths per day at the beginning, it was reduced to a few deaths per day.

[0066] From the above case analysis, it can be concluded that this set of quan...

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Abstract

The invention discloses an overall solution technical scheme for precise prevention and control of largemouth bass ranavirus of mandarin fish. According to the scheme, a paramagnetic particle method virus DNA / RNA nucleic acid extraction kit (LBT-VDEK) is adopted, a 16-hole fluorescent quantitative PCR instrument (LBT-16QPCR) and matched equipment thereof are utilized, a PCR-fluorescent probe technology is adopted, and specific DNA nucleic acid fragments of the largemouth bass ranavirus of mandarin fish are quantitatively detected, so that the method is simple, convenient and rapid, good in specificity and high in sensitivity; and a modern traditional Chinese medicine technology and a micro-ecological technology are adopted, the mandarin fish carrying the largemouth bass ranavirus is prevented and controlled, the death rate can be effectively reduced, accurate prevention and control are achieved, the problem of the largemouth bass ranavirus disease of mandarin fish is comprehensively, systematically and integrally solved, and the method has very wide application prospects and very high practical value.

Description

technical field [0001] The invention relates to aquatic animal disease prevention and control technology, and is mainly an overall technical solution for precise prevention and control of mandarin fish and frog iridescent virus diseases. Background technique [0002] Mandarin fish is a famous freshwater cultured species in my country. With the success of mandarin fish artificial breeding and nursery technology, mandarin fish aquaculture has developed rapidly, and at the same time, disease problems have become increasingly serious. The diseases of mandarin fish mainly include viral diseases, bacterial diseases and parasitic diseases. Among them, viral diseases are prevalent rapidly and have a high incidence rate, and there are no effective control measures at present, which seriously affects the development of mandarin fish aquaculture. In recent years, large-scale explosive epidemics have occurred in farmed mandarin fish, resulting in a large number of mandarin fish deaths...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6851C12Q1/6806A01K61/13C12R1/93
CPCC12Q1/701C12Q1/6851C12Q1/6806A01K61/13C12Q2531/113C12Q2563/107C12Q2523/308Y02A40/81
Inventor 黄志坚黄文文袁永青陈总会
Owner 广州力必拓生物科技有限公司
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