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Application of oleic acid in animal mammary gland development promotion

A technology of mammary gland development and oleic acid, which is applied in application, animal feed, medical preparations containing active ingredients, etc., can solve problems affecting the promotion and application of oleic acid, achieve good promotion and application prospects, high physiological activity, and promote cell proliferation Effect

Active Publication Date: 2017-09-08
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows scientists to study how animals make their own blood more than they normally produce from ingesting certain types of fat called oil with which it'll develop new ones like cow lungs when fed them up properly (fat). By doing this experimentally we found that supplemented with oleate did help promote growth of these tiny branchings and terminal breast buds while also increasing the percentage of total white blood cells in spleen tissue. These results showed that even very low levels of calcium could lead to enhanced production of mature cows.

Problems solved by technology

This patented describes how certain substances called phytate may help control excessive lipemia in cats caused by lacked carbohydrate supply from their mothers' nurses through feedings. Phytase helps break down fatted cow tissue without losing them while promoting better absorption of calcium and iron. Additionally, this process could lead to reduced fertility rates among young lambling females due to poor suckliness.

Method used

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  • Application of oleic acid in animal mammary gland development promotion
  • Application of oleic acid in animal mammary gland development promotion
  • Application of oleic acid in animal mammary gland development promotion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Oleic acid promotes the proliferation of porcine primary mammary epithelial cells

[0033] 1. In order to study the effect of oleic acid (OA) on the proliferation of mammary gland epithelial cells, the experiment first took pig primary mammary gland epithelial cells as the research object:

[0034] (1) Porcine primary mammary epithelial cells were cultured in DMEM-F12 medium containing 10% fetal bovine serum;

[0035] (2) Porcine primary mammary epithelial cells were treated with oleic acid (5, 10, 20, 50, 100 μM); the solvent of different concentrations of oleic acid was absolute ethanol.

[0036] (3) Cells were collected, and the cell proliferation phenotype was detected by MTT.

[0037] 2. The results are attached figure 1 shown. The results showed that 5-100 μM oleic acid could significantly ( P <0.05) to promote the proliferation of porcine mammary epithelial cells.

Embodiment 2

[0038] Example 2 Oleic acid promotes the proliferation of mouse mammary epithelial cells

[0039] 1. In order to further study the effect of oleic acid (OA) on the proliferation of mammary epithelial cells, mammary epithelial cells HC11 were used as the research object, and HC11 cells were cultured in RPMI-1640 medium containing 10% fetal bovine serum. The above HC11 cells were treated with different concentrations of oleic acid (12.5, 25, 50, 100, 200 μM).

[0040] (1) The effect of oleic acid on the proliferation of mammary epithelial cells was determined by MTT detection method.

[0041] The results are attached figure 2 As shown in A. The results showed that oleic acid promoted the proliferation of HC11 cells in a dose-dependent manner, and the effect of promoting cell proliferation was the best at 100 μM and 200 μM.

[0042] (2) HC11 cells were treated with 100 μM oleic acid for 4 days, and the proportion of HC11 cells in S phase was detected by EdU.

[0043] The res...

Embodiment 3

[0049] Example 3 The effect of adding 2% oleic acid on the mammary gland development of puberty mice in the diet

[0050] 1. Experimental method

[0051] (1) Animal feeding conditions: 1 animal / box, single-breeding; feeding temperature and humidity: 20-26°C, 40-70%; 12 h: 12 h day and night intermittent lighting; the conditions of the breeding room are always kept stable to ensure the experiment reliability of the results. Free access to food and water, feed and drinking water were provided by the Guangdong Provincial Medical Experimental Animal Center; Quarantine: The purchased SPF grade C57BL / 6J mice were pre-fed for 7 days. Animals were checked once a day during this period.

[0052] (2) Animal grouping and treatment: 4-week-old mice were randomly divided into normal control group and oleic acid group, 10 in each group, totally 2 groups. Mice in the normal control group were fed normal diets, mice in the oleic acid group were fed normal diets supplemented with 2% oleic a...

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Abstract

The invention discloses application of oleic acid in animal mammary gland development promotion and provides new application of the oleic acid. Cell experiments and animal experiments prove that the oleic acid can effectively promote the proliferation of pig primary mammary gland epithelial cells and mouse mammary gland epithelial cells HC11 and can increase the number of in-vivo mouse mammary gland duct branches and TEB to promote animal mammary gland development. The application has the advantages that the oleic acid is rich in source, free of substances harmful to animals and human being, safe and easy to obtain; in addition, the adding amount of the oleic acid in the application is low, and the oleic acid serving as the nutrition regulation substance can be directly added into animal feed, food or drugs and is safe, effective, free of toxic and side effects and promising in popularization and application prospect in the animal mammary gland development promotion.

Description

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Claims

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Application Information

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Owner SOUTH CHINA AGRI UNIV
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