Experimental method for promoting animal mammary gland development by using vitamin B3

Abstract

The invention discloses an experimental method for promoting animal mammary gland development by using vitamin B3. The method includes the following steps: S1, selecting 20 pregnant female mice, performing single culture, performing 12h:12h day and night discontinuous lighting, and performing free feeding and drinking; S2, taking care born mice by mother mice for four weeks after pregnant mice delivery, performing artificial weaning on the mice, and dividing the mice into two groups including a NT group (10) and a vitamin B3 group (10), wherein the feeding conditions of the NT group remain unchanged, and 0.5% of the vitamin B3 is added into daily feed of the vitamin B3 group; and S3, after the mice are fed for three weeks, killing the mice by cervical dislocation, and taking fourth pairs of mammary glands of the mice for Whole mount staining. According to the method for promoting the adolescent mammary gland development by using the vitamin B3, through in-vivo and in-vitro experiments,the proliferation and cyclin expression of EPH4EV cells and mouse mammary glands are detected, the molecular biology is used to comprehensively evaluate the effects of the vitamin B3 on the EPH4EV proliferation and mouse mammary gland development, and results show that the vitamin B3 can significantly promote the expression of cell proliferation markers in the cells and mouse mammary glands, thereby promoting the cell proliferation and mammary gland development.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to an experimental method for niacin to promote animal mammary gland development. Background technique [0002] The mammary gland is the place for producing and secreting milk, and it is also the only glandular organ for mammals to feed their offspring. Therefore, the degree of mammary gland development directly determines the growth status of young animals. In the production process, the mammary gland growth retardation of dairy cows will lead to a serious shortage of milk production during lactation, which can cause direct economic losses to the animal husbandry industry, and will also indirectly affect the breeding of dairy cows and further affect the development of animal husbandry. The study of structure and function has not been paid much attention, and the related research is still less. Therefore, it is of great significance to study the pubertal mammary gland development ...

AI Technical Summary

Problems solved by technology

However, there are no reports on the effects of niacin on the structure and function of animal mammary glands, which affects the further promotion and application of niacin.

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