Seedling budding method for rubber tree minitype bud sticks
A technique for budding of seedlings and rubber trees, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of inconvenient bud picking and pruning, and achieve the goals of shortening the cultivation cycle, having more buds, and cutting and putting into production in advance Effect
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[0010] The following examples illustrate the present invention in further detail:
[0011] Rootstock cultivation period: in the rubber seed maturity season, collect fresh seeds and germinate on the sand bed for 18-22 days, select seedlings about 20-30 cm in height and in the extended leaf stage, and remove the seedlings from the sand bed. Pull up, rinse with water to remove the sand at the base and roots of the seedlings, and soak the roots of the seedlings in a container filled with shallow water to prepare for budding.
[0012] During the cultivation of mini-sprouts: first take rubber anthers as explants, add 2.4-D0.5-1mg / l, kinetin 0.5-1mg / l, naphthalene acetic acid 1-2mg / l, coconut water to MS basic medium 5%, sucrose concentration is 70-100g / l on the medium to induce callus; MS basic medium supplemented with kinetin 0.2-1mg / l, naphthaleneacetic acid 0.1-0.2mg / l, coconut water 5%, activated charcoal 1g / l, induce embryoid bodies on the medium with a sucrose concentration of 70...
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