Method for efficiently regenerating Sapium sebiferum leaf disc

A high-efficiency regeneration technology of tallow tree leaves, applied in the biological field, can solve the problems of weak genetic research of tallow tallow, relying on tissue culture regeneration system, difficult to meet breeding needs, etc., and achieve the effect of cultivating varieties, short cultivation period and convenient operation

Inactive Publication Date: 2014-09-03
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
View PDF2 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the genetic basis of Chinese tallow tree is weak, and the breeding work is still blank. At the same time, because Chinese tallow tree is a perennial woody plant, the breeding of its varieties generally has problems such as long generation cycle and high genet

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for efficiently regenerating Sapium sebiferum leaf disc
  • Method for efficiently regenerating Sapium sebiferum leaf disc
  • Method for efficiently regenerating Sapium sebiferum leaf disc

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] The embodiments of the present invention are described in detail below. This embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operating procedures are provided, but the protection scope of the present invention is not limited to the following implementation example.

[0026] A method for efficient regeneration of tallow leaf discs provided in this embodiment comprises the following steps:

[0027] (1) Get the leaves of Chinese tallow aseptic seedlings, cut into multiple 0.3 × 0.5cm 2 Leaf disks of the size, such as figure 1 shown; wherein, the leaves are preferably taken from the fully expanded leaves of Chinese tallow tree aseptic seedlings obtained through tissue culture of Chinese tallow tree plant stems for 30 to 50 days;

[0028] (2) A plurality of leaf discs were inoculated on the cluster bud induction medium for culturing, the leaf discs were inoculated on the cluster b...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for efficiently regenerating a Sapium sebiferum leaf disc. The method comprises the following steps: cutting leaves of aseptic Sapium sebiferum seedlings into leaf discs, and conducting induction culturing on the leaf discs through a cluster bud induction culture medium to form cluster buds; conducting micropropagation and elongation on the cluster buds in a micropropagation and elongation culture medium; and after the buds grow to 2-3cm, transferring into a rooting culture medium for rooting culturing, thus obtaining strong Sapium sebiferum test-tube plantlets after 25-30 days. Compared with the prior art, the method has the following advantages that raw materials are easily available and abundant, the regeneration efficiency is high, the culture period is short, hormone components added to the culture mediums are simple, the operation is convenient, and the like, and the method can realize fast propagation of excellent wild single plants, and keep the germplasm characteristics of a female parent, thus realizing the breeding of variety, laying a foundation for the genetic transformation of Sapium sebiferum, being beneficial to quickening the genetic improvement of Sapium sebiferum, and providing technical support for the fast propagation of excellent anaphase Sapium sebiferum plant systems in the later period.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for efficiently regenerating tallow leaf discs. Background technique [0002] Sapium sebiferum (Sapium sebiferum) is a perennial deciduous tree of the Euphorbiaceae Sapium genus. Chinese tallow tree has strong adaptability to soil. It can be used in red soil, yellow soil, yellow-brown soil, purple soil, brown soil and other soils, in soils with different textures from sand to clay, and in acidic, neutral or slightly alkaline soils. It can grow and is one of the arbor species with strong salt resistance. Chinese tallow tree requires high soil moisture and can tolerate short-term waterlogging. At the same time, it has certain wind resistance and is more resistant to dryness and early barrenness. In addition, Chinese tallow tree has strong resistance to poisonous hydrogen fluoride gas. Chinese tallow tree is widely distributed in tropical and subtropical regions of my count...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
Inventor 吴丽芳侯金艳沈羊城赵薇微吴影杨鸣雷刘文博毛颖基黄胜威
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap