Method for efficiently regenerating Sapium sebiferum leaf disc

Abstract

The invention discloses a method for efficiently regenerating a Sapium sebiferum leaf disc. The method comprises the following steps: cutting leaves of aseptic Sapium sebiferum seedlings into leaf discs, and conducting induction culturing on the leaf discs through a cluster bud induction culture medium to form cluster buds; conducting micropropagation and elongation on the cluster buds in a micropropagation and elongation culture medium; and after the buds grow to 2-3cm, transferring into a rooting culture medium for rooting culturing, thus obtaining strong Sapium sebiferum test-tube plantlets after 25-30 days. Compared with the prior art, the method has the following advantages that raw materials are easily available and abundant, the regeneration efficiency is high, the culture period is short, hormone components added to the culture mediums are simple, the operation is convenient, and the like, and the method can realize fast propagation of excellent wild single plants, and keep the germplasm characteristics of a female parent, thus realizing the breeding of variety, laying a foundation for the genetic transformation of Sapium sebiferum, being beneficial to quickening the genetic improvement of Sapium sebiferum, and providing technical support for the fast propagation of excellent anaphase Sapium sebiferum plant systems in the later period.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for efficiently regenerating tallow leaf discs. Background technique [0002] Sapium sebiferum (Sapium sebiferum) is a perennial deciduous tree of the Euphorbiaceae Sapium genus. Chinese tallow tree has strong adaptability to soil. It can be used in red soil, yellow soil, yellow-brown soil, purple soil, brown soil and other soils, in soils with different textures from sand to clay, and in acidic, neutral or slightly alkaline soils. It can grow and is one of the arbor species with strong salt resistance. Chinese tallow tree requires high soil moisture and can tolerate short-term waterlogging. At the same time, it has certain wind resistance and is more resistant to dryness and early barrenness. In addition, Chinese tallow tree has strong resistance to poisonous hydrogen fluoride gas. Chinese tallow tree is widely distributed in tropical and subtropical regions of my count...

AI Technical Summary

Problems solved by technology

At present, the research on the genetic basis of Chinese tallow tree is weak, and the breeding work is still blank. At the same time, because Chinese tallow tree is a perennial woody plant, the breeding of its varieties generally has problems such as long generation cycle and high genetic heterozygosity. Conventional breeding is difficult to meet the breeding needs.

The molecular breeding method based on transgenic technology can greatly speed up the breeding process to a certain extent, but it needs to rely on an efficient and stable tissue culture regeneration system

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