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Seed-cultivating method of blueberry by means of tissue cultivation and micropropagation

A technology of blueberry and tissue culture, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of slow speed, inability to meet the increasing needs of blueberry seedlings, low survival rate, etc., and achieve increased reproduction coefficient, Promote cell expansion and elongation, promote the effect of cell division

Active Publication Date: 2010-10-27
长春百瑞蓝莓科技发展有限公司
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  • Summary
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  • Application Information

AI Technical Summary

Problems solved by technology

Using conventional hard branch and green branch cuttings has low survival rate and slow speed, which cannot meet the growing demand of the market for the number of blueberry seedlings

Method used

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Examples

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Embodiment

[0009] The present invention relates to a blueberry tissue culture and micropropagation seedling raising method, in which young blueberry shoots are collected from the field during the growing season, and the surface is sterilized with 0.1% mercury chloride by mass percentage for 5 to 7 minutes under aseptic conditions, and then rinsed with sterile water for 3 times Finally, cut into strips and inoculate 1 to 2 bud stem segments into the primary induction medium WPM+ZT 3.0mg / L+NAA 0.02mg / L+sucrose 30g / L+agar 6.5g / L, pH 5.4. After 2 to 3 generations of culture, a sterile, vigorously growing and branched tissue culture clone is obtained. In the stage of subculture proliferation of blueberry tissue culture seedlings, the proliferation medium WPM+ZT0.5-1.5mg / L+CPPU 0.05-1.0mg / L+sucrose 30g / L+agar 6.5g / L, pH 5.4. One subculture cycle (repeat the same culture process) every 50-60 days. In the 2-3 subculture cycles before the blueberries are transplanted out of the bottle, the seedl...

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PUM

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Abstract

The invention provides a seed-cultivating method of the blueberry by means of tissue cultivation and micropropagation, which comprises the following steps of: picking young sprout of the blueberry from fields in the growth season; sterilizing the surface of the young sprout under the aseptic condition; washing the sterilized young sprout with the sterile water; cutting the washed young sprout into a stem section with 1-2 bud(s); characteristically inoculating the stem section with 1-2 bud(s) on a first-generation inducting culture medium 3.0mg / L of WPM+ZT+0.02mg / L of NAA+30g / L of saccharose+6.5g / L of agar, wherein the PH value of the culture medium is 5.4; cultivating for 2-3 generations to obtain a tissue cultivation clone which is sterile and exuberantly grown and has multiple branches; using a cultivating culture medium 0.5-1.5mg / L of WPM+ZT+0.05-1.0mg / L of CPPU+30g / L of saccharose+6.5g / L of agar at the subculture and multiplication culture stage of the tissue cultivating seed of the blueberry, wherein the PH value of the culture medium is 5.4; and subculturing at a time every 50-60 days, thereby improving the propagation line by 5-10 times.

Description

technical field [0001] The invention relates to a blueberry propagation method, in particular to a blueberry tissue culture and micropropagation seedling raising method. Background technique [0002] Blueberry belongs to Rhododendronaceae Vaccinium shrub, is a kind of small berry fruit tree, and its fruit has very high nutritional value and economic value. At present, the demand for blueberries in the market is constantly increasing, but the planting industry of blueberries in my country has just started, and various places are in urgent need of a large number of seedlings of good varieties when establishing plantations. Adopt conventional hard branch, green branch cutting survival rate is low, and speed is slow, can't satisfy the demand that the market increases day by day to blueberry seedling quantity. The rapid propagation of blueberry seedlings by tissue culture technology is the most effective way. The seedlings produced by tissue culture and micropropagation are of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 杜丽华
Owner 长春百瑞蓝莓科技发展有限公司
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