Micropropagation breeding method for dendrobium officinale

Abstract

The invention provides a micropropagation breeding method for dendrobium officinale. Protocorm is generated through induction of dendrobium officinale seeds, protoemm-like bodies are generated through induction of protocorm, through efficient propagation of the protoemm-like bodies, regenerated bud seedlings are generated through induction, and regenerated plants are generated through rooting of the bud seedlings. When each of the regenerated plants grows to have 5-6 leaves, seedling hardening is started, plantlets can be transplanted out after a week of seedling hardening, and the plantlets grow normally. The protoemm-like bodies are obtained through micropropagation of the dendrobium officinale protocorm, the number is multiplied, the number of future seedlings is rapidly increased, the rapid propagation process of the dendrobium officinale seedlings is accelerated, the propagation efficiency of the dendrobium officinale seedlings is remarkably improved, the maximum propagation coefficient can reach 23, and therefore a large amount of manpower and a large number of material resources are saved. The efficient micropropagation breeding method is provided for dendrobium officinale, and reference can be provided for breeding of other orchidaceae plants.

Description

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AI Technical Summary

Benefits of technology

This new method allows for faster growth rates by promoting specific changes from dendrosis bacteria called Actinobacillus brevis (formerly known as Rhodocystis jirovea) during their colonies' development cycle. These modifications can lead to improved germination performance or increased reproductive potential compared to previous methods that had been previously used.

Problems solved by technology

This patented technical problem addressed in this patents describes how quickly growing new generations of Lithycodon grandifera from natural sources like Japanese or Korean bush can provide beneficial health benefits without causing harmful side effectors during their development process.

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